Copper(II)/Copper(I)-Catalyzed Aza-Michael Addition/Click Reaction of in Situ Generated α-Azidohydrazones: Synthesis of Novel Pyrazolone−Triazole Framework

2010 ◽  
Vol 12 (3) ◽  
pp. 468-471 ◽  
Author(s):  
Orazio A. Attanasi ◽  
Gianfranco Favi ◽  
Paolino Filippone ◽  
Fabio Mantellini ◽  
Giada Moscatelli ◽  
...  
Keyword(s):  
Michael Addition ◽  
Click Reaction

ChemInform Abstract: Copper(II)/Copper(I)-Catalyzed Aza-Michael Addition/Click Reaction of in situ Generated α-Azidohydrazones: Synthesis of Novel Pyrazolone-Triazole Framework.

ChemInform ◽  
2010 ◽  
Vol 41 (26) ◽  
pp. no-no
Author(s):  
Orazio A. Attanasi ◽  
Gianfranco Favi ◽  
Paolino Filippone ◽  
Fabio Mantellini ◽  
Giada Moscatelli ◽  
...  
Keyword(s):  
Michael Addition ◽  
Click Reaction

DNA‐Compatible Click Reaction Employing In Situ Generated Azides from Boronic Acids

2021 ◽  
Vol 1 (5) ◽  
Author(s):  
Yulong An ◽  
Hao Yan ◽  
Zhenzhen Dong ◽  
Alexander L. Satz
Keyword(s):  
Click Reaction ◽  
Boronic Acids

ent-Kaurane Diterpenoids from Chinese Liverworts and Their Antitumor Activities through Michael Addition As Detected in Situ by a Fluorescence Probe

2015 ◽  
Vol 58 (9) ◽  
pp. 3944-3956 ◽  
Author(s):  
Zhaomin Lin ◽  
Yanxia Guo ◽  
Yanhui Gao ◽  
Shuqi Wang ◽  
Xiaoning Wang ◽  
...  
Keyword(s):  
Michael Addition ◽  
Fluorescence Probe ◽  
Antitumor Activities

scholarly journals Deciphering the in vivo Dynamic Proteomics of Mesenchymal Stem Cells in Critical Limb Ischemia

2021 ◽  
Vol 9 ◽  
Author(s):  
Yipeng Du ◽  
Xiaoting Li ◽  
Wenying Yan ◽  
Zhaohua Zeng ◽  
Dunzheng Han ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Protein Synthesis ◽  
Critical Limb Ischemia ◽  
Click Reaction ◽  
Limb Ischemia ◽  
Trna Synthetase ◽  
Doppler Imaging

ObjectiveRegenerative therapy using mesenchymal stem cells (MSC) is a promising therapeutic method for critical limb ischemia (CLI). To understand how the cells are involved in the regenerative process of limb ischemia locally, we proposed a metabolic protein labeling method to label cell proteomes in situ and then decipher the proteome dynamics of MSCs in ischemic hind limb.Methods and ResultsIn this study, we overexpressed mutant methionyl-tRNA synthetase (MetRS), which could utilize azidonorleucine (ANL) instead of methionine (Met) during protein synthesis in MSCs. Fluorescent non-canonical amino-acid tagging (FUNCAT) was performed to detect the utilization of ANL in mutant MSCs. Mice with hindlimb ischemia (HLI) or Sham surgery were treated with MetRSmut MSCs or PBS, followed by i.p. administration of ANL at days 0, 2 6, and 13 after surgery. FUNCAT was also performed in hindlimb tissue sections to demonstrate the incorporation of ANL in transplanted cells in situ. At days 1, 3, 7, and 14 after the surgery, laser doppler imaging were performed to detect the blood reperfusion of ischemic limbs. Ischemic tissues were also collected at these four time points for histological analysis including HE staining and vessel staining, and processed for click reaction based protein enrichment followed by mass spectrometry and bioinformatics analysis. The MetRSmut MSCs showed strong green signal in cell culture and in HLI muscles as well, indicating efficient incorporation of ANL in nascent protein synthesis. By 14 days post-treatment, MSCs significantly increased blood reperfusion and vessel density, while reducing inflammation in HLI model compared to PBS. Proteins enriched by click reaction were distinctive in the HLI group vs. the Sham group. 34, 31, 49, and 26 proteins were significantly up-regulated whereas 28, 32, 62, and 27 proteins were significantly down-regulated in HLI vs. Sham at days 1, 3, 7, and 14, respectively. The differentially expressed proteins were more pronounced in the pathways of apoptosis and energy metabolism.ConclusionIn conclusion, mutant MetRS allows efficient and specific identification of dynamic cell proteomics in situ, which reflect the functions and adaptive changes of MSCs that may be leveraged to understand and improve stem cell therapy in critical limb ischemia.

The Thiol-Michael Addition Click Reaction: A Powerful and Widely Used Tool in Materials Chemistry

2013 ◽  
Vol 26 (1) ◽  
pp. 724-744 ◽  
Author(s):  
Devatha P. Nair ◽  
Maciej Podgórski ◽  
Shunsuke Chatani ◽  
Tao Gong ◽  
Weixian Xi ◽  
...  
Keyword(s):  
Michael Addition ◽  
Click Reaction ◽  
Materials Chemistry

Dearomatization-Rearomatization Strategy of Tyrosine for Peptides/Protein Modification through Thiol-Addition Reactions

2021 ◽  
Author(s):  
Pengxin Wang ◽  
Yulian Cheng ◽  
Chunlei Wu ◽  
Ruixiang Luo ◽  
Caibin Ma ◽  
...  
Keyword(s):  
Michael Addition ◽  
Protein Modification ◽  
Addition Reactions

We have developed a dearomatization-rearomatization strategy for modification of peptides/protein through thiol-Michael addition to the electrophilic cyclohexadienone intermediate in situ generated by oxidation of tyrosine. This strategy enriches the conjugation...

Cu-Catalyzed one-pot synthesis of thiochromeno-quinolinone and thiochromeno-thioflavone via oxidative double hetero Michael addition using in situ generated nucleophiles

2020 ◽  
Vol 56 (62) ◽  
pp. 8826-8829
Author(s):  
Nallappan Sundaravelu ◽  
Govindasamy Sekar
Keyword(s):  
Michael Addition ◽  
One Pot ◽  
One Pot Synthesis

A copper catalyzed three-component synthesis of π-conjugated tetracyclic thiochromeno-quinolinone and thiochromeno-thioflavone was established via oxidative double hetero Michael addition using in situ generated nucleophiles.

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