One- and Two-Photon Live Cell Imaging Using a Mutant SNAP-Tag Protein and Its FRET Substrate Pairs

2011 ◽  
Vol 13 (16) ◽  
pp. 4160-4163 ◽  
Author(s):  
Chong-Jing Zhang ◽  
Lin Li ◽  
Grace Y. J. Chen ◽  
Qing-Hua Xu ◽  
Shao Q. Yao
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Two Photon

scholarly journals In vivo live-cell imaging in plant tissues by two-photon excitation microscopy

2014 ◽  
Vol 26 (1) ◽  
pp. 25-30
Author(s):  
Yoko Mizuta ◽  
Daisuke Kurihara ◽  
Tetsuya Higashiyama
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Plant Tissues ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

High Speed Two Photon Excitation Microscopy in Live Cell Imaging using Image Correlation Spectroscopy (ICS)

2001 ◽  
Vol 7 (S2) ◽  
pp. 22-23
Author(s):  
P. W. Wiseman ◽  
J. C. Bouwer ◽  
S. Peltier ◽  
M. H. Ellisman
Keyword(s):  
Live Cell Imaging ◽  
High Speed ◽  
Cell Imaging ◽  
Live Cell ◽  
Image Correlation ◽  
Scanning System ◽  
Optical Sectioning ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

For live-cell imaging, two-photon excitation microscopy (TPEM) is proving to be a significant technological advancement. The unique features offered by TPEM are the ability to image thick sections, excellent optical sectioning capabilities, low damage to living cells, and less out of focus fluorescence and out of focus photobleaching. of these features, the most useful for the biological microscopist, is optical sectioning. Optical sectioning is an intrinsic property of the two-photon process, whereby, two infrared (IR) photons are absorbed quickly to excite a single UV/blue transition. The probability for exciting a two photon transition is proportional to the instantaneous excitation intensity squared. Therefore, for a focused laser beam, only light at the focal point of the excitation beam excites a fluorescent transition. Thus, the need for confocal apertures and time consuming deconvolution algorithms are, for the most part, eliminated.We have continued to develop and enhance our ability to perform high-speed, two-photon excitation fluorescence microscopy. in 1998, we successfully deployed a prototype, video-rate twophoton laser scanning system (30 frames/sec or faster at reduced scan width) developed with support from Nikon Corporation. That system was built upon a Nikon RCM 8000 confocal microscope.

Bionanoprobes with Excellent Two-Photon-Sensitized Eu3+ Luminescence Properties for Live Cell Imaging

2010 ◽  
Vol 16 (29) ◽  
pp. 8647-8651 ◽  
Author(s):  
Guangsheng Shao ◽  
Rongcheng Han ◽  
Yan Ma ◽  
Minxian Tang ◽  
Fumin Xue ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Luminescence Properties ◽  
Two Photon

Construction of an orthogonal ZnSalen/Salophen library as a colour palette for one- and two-photon live cell imaging

2014 ◽  
Vol 5 (6) ◽  
pp. 2318 ◽  
Author(s):  
Da Xie ◽  
Jing Jing ◽  
Yuan-Bo Cai ◽  
Juan Tang ◽  
Juan-Juan Chen ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Two Photon ◽  
Colour Palette

A hexaphenylbenzene based AIEE active two photon probe for the detection of hydrogen sulfide with tunable self-assembly in aqueous media and application in live cell imaging

2015 ◽  
Vol 51 (85) ◽  
pp. 15570-15573 ◽  
Author(s):  
Subhamay Pramanik ◽  
Vandana Bhalla ◽  
Hwan Myung Kim ◽  
Hardev Singh ◽  
Hyo Won Lee ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Hela Cells ◽  
Self Assembly ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Aqueous Media ◽  
Live Cell ◽  
Two Photon ◽  
Self Assembled

AIEE active HPB based TPE probe exhibited modulation of self-assembled architecture in presence of H2S and was utilized for TPM imaging for detection of H2S in HeLa cells.

A Bioaccumulative Cyclometalated Platinum(II) Complex with Two-Photon-Induced Emission for Live Cell Imaging

2009 ◽  
Vol 48 (3) ◽  
pp. 872-878 ◽  
Author(s):  
Chi-Kin Koo ◽  
Ka-Leung Wong ◽  
Cornelia Wing-Yin Man ◽  
Yun-Wah Lam ◽  
Leo King-Yan So ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Two Photon

scholarly journals Silafluorene as a promising core for cell-permeant, highly bright and two-photon excitable fluorescent probes for live-cell imaging

2021 ◽  
Vol 187 ◽  
pp. 109083
Author(s):  
Marie Auvray ◽  
Frédéric Bolze ◽  
Gilles Clavier ◽  
Florence Mahuteau-Betzer
Keyword(s):  
Fluorescent Probes ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Two Photon
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