Inhibitory Effect of Macabarterin, a Polyoxygenated Ellagitannin fromMacaranga barteri, on Human Neutrophil Respiratory Burst Activity

2008 ◽  
Vol 71 (11) ◽  
pp. 1906-1910 ◽  
Author(s):  
Rostand Manfouo Ngoumfo ◽  
Georges Eric Ngounou ◽  
Chimene Victoire Tchamadeu ◽  
Muhammad Irfan Qadir ◽  
Celine Djama Mbazoa ◽  
...  
Keyword(s):  
Respiratory Burst ◽  
Human Neutrophil ◽  
Inhibitory Effect ◽  
Burst Activity ◽  
Respiratory Burst Activity ◽  
Neutrophil Respiratory Burst ◽  
Neutrophil Respiratory Burst Activity

scholarly journals Pseudomonas aeruginosa Hemolytic Phospholipase C Suppresses Neutrophil Respiratory Burst Activity

1999 ◽  
Vol 67 (5) ◽  
pp. 2371-2376 ◽  
Author(s):  
Lance S. Terada ◽  
Kristine A. Johansen ◽  
Sogol Nowbar ◽  
Adriana I. Vasil ◽  
Michael L. Vasil
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Phospholipase C ◽  
Oxidative Burst ◽  
Respiratory Burst ◽  
Burst Activity ◽  
Wild Type ◽  
Respiratory Burst Activity ◽  
P38 Kinase ◽  
Neutrophil Respiratory Burst ◽  
Neutrophil Respiratory Burst Activity

ABSTRACT Pseudomonas aeruginosa is a persistent pathogen in the airways of patients with cystic fibrosis or bronchiectasis from other causes and appears to have evolved strategies to survive the inflammatory response of the host. We hypothesized that the secreted hemolytic phospholipase C (PLC) of P. aeruginosa (PlcHR) would decrease neutrophil respiratory burst activity. We found that while intact wild-type P. aeruginosa cells stimulated moderate respiratory burst activity from human neutrophils, an isogenic mutant pseudomonas (ΔHR strain) containing a targeted deletion of the plcHR operon induced a much more robust oxidative burst from neutrophils. In contrast, a second pseudomonas mutant (ΔN) containing a disruption in the gene encoding the nonhemolytic PLC (PlcN) was not different from the wild type in stimulating neutrophil O2 ·−production. Readdition of purified PlcHR to the ΔHR strain suppressed neutrophil O2 ·−production to levels stimulated by wild-type bacteria. Interestingly, purified PlcHR decreased phorbol myristate acetate (PMA)- but not formyl methionyl-leucyl-proline (fMLP)-induced respiratory burst activity, suggesting interference by PlcHR with a protein kinase C (PKC)-specific signaling pathway. Accordingly, the PKC inhibitor bisindolylmaleimide inhibited the oxidative burst induced by either PMA or intact pseudomonas, but not by fMLP, whereas the p38 kinase inhibitor SB-203580 fully inhibited the respiratory burst induced by fMLP or the PlcHR-replete wild-type bacteria, but not PMA or the PlcHR-deficient ΔHR bacterial mutant. We conclude that expression of PlcHR by P. aeruginosa suppresses bacterium-induced neutrophil respiratory burst by interfering with a PKC-dependent, non-p38 kinase-dependent pathway.

Oat ??-Glucan Effects on Neutrophil Respiratory Burst Activity following Exercise

2007 ◽  
Vol 39 (4) ◽  
pp. 639-644 ◽  
Author(s):  
E. ANGELA MURPHY ◽  
J. MARK DAVIS ◽  
ADRIENNE S. BROWN ◽  
MARTIN D. CARMICHAEL ◽  
ABDUL GHAFFAR ◽  
...  
Keyword(s):  
Respiratory Burst ◽  
Burst Activity ◽  
Respiratory Burst Activity ◽  
Neutrophil Respiratory Burst ◽  
Neutrophil Respiratory Burst Activity
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