Multitarget Affinity/Specificity Screening of Natural Products:  Finding and Characterizing High-Affinity Ligands from Complex Mixtures by Using High-Performance Mass Spectrometry

2003 ◽  
Vol 66 (9) ◽  
pp. 1186-1190 ◽  
Author(s):  
Lendell L. Cummins ◽  
Shuo Chen ◽  
Larry B. Blyn ◽  
Kristin A. Sannes-Lowery ◽  
Jared J. Drader ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Natural Products ◽  
High Performance ◽  
Complex Mixtures ◽  
Affinity Ligands ◽  
High Affinity

Compositional Analysis of Organosolv Poplar Lignin by Using High-Performance Liquid Chromatography/High-resolution Multi-stage Tandem Mass Spectrometry

2021 ◽  
Author(s):  
Jifa Zhang ◽  
Yuan Jiang ◽  
Leah F Easterling ◽  
Anton Anster ◽  
Wanru Li ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Compositional Analysis ◽  
Complex Mixtures ◽  
Tandem Mass ◽  
Multi Stage ◽  
The Individual ◽  
Environmentally Friendly Process

Organosolv treatment is an efficient and environmentally friendly process to degrade lignin into small compounds. The capability of characterizing the individual compounds in the complex mixtures formed upon organosolv treatment...

scholarly journals Diazirine-functionalized mannosides for photoaffinity labeling: trouble with FimH

2018 ◽  
Vol 14 ◽  
pp. 1890-1900 ◽  
Author(s):  
Femke Beiroth ◽  
Tomas Koudelka ◽  
Thorsten Overath ◽  
Stefan D Knight ◽  
Andreas Tholey ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Photoaffinity Labeling ◽  
Extensive Study ◽  
Covalent Modification ◽  
Bacterial Protein ◽  
High Affinity ◽  
Limited Success ◽  
Receptor Interactions ◽  
Interdisciplinary Methodology

Photoaffinity labeling is frequently employed for the investigation of ligand–receptor interactions in solution. We have employed an interdisciplinary methodology to achieve facile photolabeling of the lectin FimH, which is a bacterial protein, crucial for adhesion, colonization and infection. Following our earlier work, we have here designed and synthesized diazirine-functionalized mannosides as high-affinity FimH ligands and performed an extensive study on photo-crosslinking of the best ligand (mannoside 3) with a series of model peptides and FimH. Notably, we have employed high-performance mass spectrometry to be able to detect radiation results with the highest possible accuracy. We are concluding from this study that photolabeling of FimH with sugar diazirines has only very limited success and cannot be regarded a facile approach for covalent modification of FimH.

Lipids of aquatic sediments, recent and ancient

1979 ◽  
Vol 293 (1400) ◽  
pp. 69-91 ◽  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Natural Products ◽  
Data Processing ◽  
Homologous Series ◽  
Carbon Number ◽  
Complex Mixtures ◽  
Gas Chromatography Mass Spectrometry ◽  
Aquatic Sediments ◽  
Abundance Patterns

Computerized gas chromatography - mass spectrometry (g.c.-m.s.) is now an essential tool in the analysis of the complex mixtures of lipids (geolipids) encountered in aquatic sediments, both Recent (less than 1 Ma (10 6 years) old) and ancient. Most geolipid studies have been performed in the e.i. mode at low resolution but the techniques now being applied include c.i. and h.r.m.s. The large quantities of data acquired from capillary g.c.-m.s. runs necessitate fast data acquisition and data processing, including the capability for the automatic selection and refinement of key spectra. Even so, the chemist is faced with the identification and/or recognition of at least several hundred good quality spectra from a single run. Fast routine search procedures are useful here, especially for known compounds, while classification routines based on established rules for manual interpretation can be of assistance even with novel compounds. Examples from recent studies (at Bristol) of contemporary, Recent and ancient sediments, are presented. Geolipids show abundance patterns of homologous series which, while related to those of known organisms, display many novel features, including extensive carbon number ranges and stereospecific distributions. Additionally, certain carbon skeleton types, commonly thought to be rare as natural products, are major components of geolipid fractions, presumably reflecting the composite inputs and early microbial diagenetic activity.

Screening for High-Affinity Ligands to theSrcSH2 Domain Using Capillary Isoelectric Focusing-Electrospray Ionization Ion Trap Mass Spectrometry

1998 ◽  
Vol 70 (22) ◽  
pp. 4761-4770 ◽  
Author(s):  
Yelena V. Lyubarskaya ◽  
Steven A. Carr ◽  
Damien Dunnington ◽  
William P. Prichett ◽  
Seth M. Fisher ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Isoelectric Focusing ◽  
Ion Trap ◽  
Ion Trap Mass Spectrometry ◽  
Capillary Isoelectric Focusing ◽  
Affinity Ligands ◽  
High Affinity

Removal of Pesticides by Organic Montmorillonite Composites

2014 ◽  
Vol 875-877 ◽  
pp. 262-266 ◽  
Author(s):  
Xue Si Wang ◽  
Hai Bo Ma ◽  
Guo Bing Li ◽  
Jia Bei He ◽  
Ping Na
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Promising Material ◽  
Liquid Chromatography Mass Spectrometry ◽  
Pesticide Pollution ◽  
High Affinity ◽  
Pollution Treatment ◽  
Removal Experiments

The removal of pesticides (quintozene, α-BHC, Aldrin and pp′-DDT) by phenyltrimethylammonium bromide (PTMAB) modified montmorillonites(PTMAB-MMT) was investigated. The results showed that these four kinds of pestcides have high affinity with PTMAB-MMT. The composites were characterized by FTIR and XRD. Further, the removal experiments of pesticides in ginseng extracts were carried out. The Ginsenosides were analyzed by high performance liquid chromatography/mass spectrometry showed that the loss of Ginsenosides was less than 4%. It can be concluded that the PTMAB-MMT is a promising material in pesticide pollution treatment

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