Fullerene Nanoparticles Selectively Enter Oxidation-Damaged Cerebral Microvessel Endothelial Cells and Inhibit JNK-Related Apoptosis

ACS Nano ◽  
2009 ◽  
Vol 3 (11) ◽  
pp. 3358-3368 ◽  
Author(s):  
Fang Lao ◽  
Long Chen ◽  
Wei Li ◽  
Cuicui Ge ◽  
Ying Qu ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Fullerene Nanoparticles ◽  
Microvessel Endothelial Cells ◽  
Cerebral Microvessel

scholarly journals High glucose conditioned neonatal astrocytes results in impaired mitogenic activity in cerebral microvessel endothelial cells in co-culture

Heliyon ◽  
2019 ◽  
Vol 5 (5) ◽  
pp. e01795 ◽  
Author(s):  
Susan Cohen ◽  
Qiuli Liu ◽  
Matthew Wright ◽  
Jodi Garvin ◽  
Kevin Rarick ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
High Glucose ◽  
Mitogenic Activity ◽  
Microvessel Endothelial Cells ◽  
Cerebral Microvessel

scholarly journals Effects of copper nanoparticles on rat cerebral microvessel endothelial cells

Nanomedicine ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. 835-846 ◽  
Author(s):  
William J Trickler ◽  
Susan M Lantz ◽  
Amanda M Schrand ◽  
Bonnie L Robinson ◽  
Glenn D Newport ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Copper Nanoparticles ◽  
Microvessel Endothelial Cells ◽  
Cerebral Microvessel

Evaluation of brain-targeted chitosan nanoparticles through blood–brain barrier cerebral microvessel endothelial cells

2017 ◽  
Vol 34 (7) ◽  
pp. 659-666 ◽  
Author(s):  
Adem Sahin ◽  
Digdem Yoyen-Ermis ◽  
Secil Caban-Toktas ◽  
Utku Horzum ◽  
Yesim Aktas ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Blood Brain Barrier ◽  
Chitosan Nanoparticles ◽  
Brain Barrier ◽  
Blood Brain ◽  
Microvessel Endothelial Cells ◽  
Cerebral Microvessel

IL-6 secreted by astroglial cells regulates Na-K-Cl cotransport in brain microvessel endothelial cells

1997 ◽  
Vol 272 (6) ◽  
pp. C1829-C1835 ◽  
Author(s):  
D. Sun ◽  
C. Lytle ◽  
M. E. O'Donnell
Keyword(s):  
Endothelial Cells ◽  
Protein Expression ◽  
Enzyme Linked Immunosorbent Assay ◽  
Astroglial Cell ◽  
Astroglial Cells ◽  
Microvessel Endothelial Cells ◽  
Brain Microvessel ◽  
Dose Dependent ◽  
Cell Conditioned Medium ◽  
Cerebral Microvessel

We showed previously that cerebral microvessel endothelial cells (CMEC) exhibit a prominent Na-K-Cl cotransporter that functions to regulate intracellular volume and may also mediate vectorial ion transport across the blood-brain barrier (BBB). Astrocytes and their conditioned media induce BBB properties of the endothelium. Our previous studies demonstrated that exposure of CMEC to astroglial cells markedly increases cotransport activity, upregulates cotransporter protein expression, and also increases cotransporter protein phosphorylation. In the present study, we evaluated the possibility that astroglial effects on the Na-K-Cl cotransporter are mediated by astroglial cell-secreted interleukin-6 (IL-6). Cotransporter activity was assessed as bumetanide-sensitive K influx, and protein expression was evaluated by Western blot analysis. Exposing CMEC to IL-6 for 48 h caused a dose-dependent stimulation of Na-K-Cl cotransport activity. Both C6 glial cell-conditioned medium (C6CM)-induced and IL-6-induced increases in cotransport activity were neutralized by anti-IL-6 antibodies. A 48-h exposure of cells to IL-6 or C6CM also resulted in increased cotransporter protein expression. Furthermore, using an enzyme-linked immunosorbent assay for IL-6, we found significant amounts of IL-6 in C6CM. These data suggest that astroglia-secreted IL-6 may mediate the observed astroglial cell effects on CMEC Na-K-Cl cotransport and further support the hypothesis that astrocytes participate in maintenance of cerebral ionic homeostasis by regulating Na-K-Cl cotransporter function at the BBB.

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