scholarly journals Gold Nanorod Based Selective Identification of Escherichia coli Bacteria Using Two-Photon Rayleigh Scattering Spectroscopy

ACS Nano ◽  
2009 ◽  
Vol 3 (7) ◽  
pp. 1906-1912 ◽  
Author(s):  
Anant K. Singh ◽  
Dulal Senapati ◽  
Shuguang Wang ◽  
Jelani Griffin ◽  
Adria Neely ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rayleigh Scattering ◽  
Gold Nanorod ◽  
Two Photon ◽  
Escherichia Coli Bacteria

scholarly journals Magnetic Ni-Doped TiO2 Photocatalysts for Disinfection of Escherichia coli Bacteria

2021 ◽  
Author(s):  
Khang Cao Nguyen ◽  
Nghia Manh Nguyen ◽  
Van Quoc Duong ◽  
Khanh Van Nguyen ◽  
Hung Manh Nguyen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Tio2 Photocatalysts ◽  
Doped Tio2 ◽  
Escherichia Coli Bacteria

scholarly journals Uji Bioaktivitas Antibakteri Senyawa murni dari Jamur Endofit Sporothrix sp Terhadap Bakteri Escherichia coli dan Staphylococcus aureus

2019 ◽  
Vol 6 (1) ◽  
pp. 37
Author(s):  
Dewi Yudiana Shinta ◽  
Yusmarini Yusmarini ◽  
Herix Sonata MS ◽  
Hilwan Yuda Teruna ◽  
Saryono Saryono
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Endophytic Fungi ◽  
Positive Control ◽  
Significant Inhibition ◽  
New Drugs ◽  
Diffusion Method ◽  
Significant Difference ◽  
Pure Compounds ◽  
Escherichia Coli Bacteria

Modern medicines that are developing now come from active ingredients isolated from plants that require large amounts of plants. The development of new drugs from endophytic fungi found obstacles in the amount of pure compounds produced. Therefore further research is needed by using endophytic fungi as a new antimicrobial producer. This study aims to see the ability or activity of pure compounds produced by Sporothrix sp endophytic fungi from Dahlia tuber (Dahlia variabilis). Test the activity of pure compounds produced by Sporothrix sp. Endophytic fungi on E. coli and Staphylococcus aureus determined by disc diffusion method. With doses of 10, 30 and 50μg/disk. In Escherichia coli bacteria doses 10 and 50μg/disk gave significant inhibition of pure compounds from isolation compared to the positive control of ciprofloxacin, which was marked by a statistically significant test result (p <0.05). In contrast to Staphylococcus aureus there was no significant difference in doses of both doses of 10.30 and 50μg/disk. Determination of pure compounds was carried out by HPLC and Infra Red Spectrophotometry.

scholarly journals Different ideas on the pathogenesis and treatment of swine edema-disease

2021 ◽  
pp. 45-48
Author(s):  
Gabriella Novotniné Dankó ◽  
Gyula Dankó †
Keyword(s):  
Escherichia Coli ◽  
Insulin Release ◽  
Urine Retention ◽  
Edema Disease ◽  
Loss Of Balance ◽  
Release Of Acetylcholine ◽  
Escherichia Coli Bacteria

Although literature data associate the reason of swine edema-disease with certain serotypes of Escherichia coli bacteria, the authors assume that the primary cause of edema is more different. Susceptible agents and factors, mostly of feed compound are involved. During the digestion of some feed-origin protein opiate-like metabolites, exorphins arise, simultaneously arrest the release of acetylcholine. Consequences of acetylcholine shortage are spasm of sphincters (mostly pylorus), intestine-dilatation, contraction of bladder-sphincter, and urine retention. The endorphins and exorphins intensify the insulin release from the pancreas, hypoglycemia evolves, which is associated with loss of balance. According to the authors in edema-disease piglet dies because of hypoglycemia.

scholarly journals GREEN SYNTHESIS OF MAGNETIC IRON NANOPARTICLES USING MEDICINAL PLANT TRIDAX PROCUMBENS LEAF EXTRACTS AND ITS APPLICATION AS AN ANTIMICROBIAL AGENT AGAINST E. COLI

2020 ◽  
pp. 34-39
Author(s):  
YOJANA Y. PATIL ◽  
VAISHNVI B. SUTAR ◽  
ARPITA P. TIWARI
Keyword(s):  
Escherichia Coli ◽  
Magnetic Nanoparticles ◽  
Iron Nanoparticles ◽  
Most Probable Number ◽  
Leaf Extracts ◽  
Probable Number ◽  
Gram Negative ◽  
E Coli ◽  
Tridax Procumbens ◽  
Escherichia Coli Bacteria

Objective: The present study was aimed at the biological synthesis of magnetic iron nanoparticles by using the plant extract of Tridax procumbens and also to study their antimicrobial property against gram-negative bacteria (Escherichia coli). Methods: The synthesis of magnetic iron nanoparticles was carried out by the co-precipitation method using biological methods like plant extract as reducing agent and capping agents are biocompatible and non-hazardous. These nanoparticles were characterized by UV-Visible spectroscopy, XRD (X-Ray Diffraction), and SEM (Scanning Electron Microscope). As well as antibacterial activity of the nanoparticles was carried out by agar well diffusion method and Most Probable Number (MPN) method against gram-negative E. coli (Escherichia coli) bacteria. Results: The average crystallite size of Magnetic Nanoparticles (MNPs) was found to be 72 nm by X-ray diffraction. The optical absorption band at wavelengths of 240 nm and 402 nm was obtained from the UV Visible spectrum. Spherical shape morphology was observed in SEM studies. The antibacterial assay clearly expressed that E. coli showed a maximum zone of inhibition (15±0.15 mm) at 2 mg/ml and 1 mg/ml concentration was found for Magnetic Nanoparticles. In the Most Probable Number (MPN) test it is seen that the bacterial count is reduced after adding synthesized NPs into the water sample. Conclusion: The results of the present study conclude that the Magnetic Nanoparticles synthesized using Tridax procumbens leaf extracts is found to be stable and show good antibacterial activity against gram-negative (Escherichia coli) bacteria.

scholarly journals SINTESIS NANOKOMPOSIT Nata de coco/TiO2/Ag DAN EFEKTIVITASNYA SEBAGAI ANTIBAKTERI TERHADAP BAKTERI Escherichia coli dan Staphylococcus aureus

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Heriyanto Tinentang ◽  
Henry F Aritonang ◽  
Harry S. J. Koleangan
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Scanning Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Chemical Reduction ◽  
Bacterial Activity ◽  
Energy Dispersive ◽  
X Ray ◽  
Escherichia Coli Bacteria ◽  
Scanning Electron

Telah dilakukan penelitian tentang kemampuan aktivitas anti bakteri untuk bakteri Staphylococcus aureus (gram positif) dan Escherichia coli (gram negatif) dengan menggunakan nanokomposit nata de coco/TiO2, nata de coco/Ag, dan nata de coco/TiO2/Ag dengan variasi konsentrasi Ag 0,5 M; 0,6 M; 0,7 M; 0,8 M dan 0,9 M  menggunakan metode reduksi kimia. Nanopartikel tersebut dikarakterisasi menggunakan X-Ray Diffractometry (XRD), scanning electron microscopy-energy dispersive X-ray spectroscopy  (SEM-EDS) dan anti bakteri. Untuk uji aktivitas antibakteri menggunakan metode kertas cakram dan dilakukan sebanyak dua kali ulangan untuk tiap-tiap sampel dan bakteri yang diuji. Hasil penelitian menunjukan, aktivitas anti bakteri nanokomposit yang paling baik dalam menghambat pertumbuhan bakteri adalah nanokomposit Nata de coco/TiO2/Ag mampu menghambat pertumbuhan bakteri Escherichiacoli dan Staphylococcusaureus, namun nanokomposit tersebut lebih efektif menghambat pertumbuhan bakteri Escherichiacoli.ABSRACT Research on the ability of anti-bacterial activity for Staphylococcus aureus (gram positive) and Escherichia coli (gram negative) bacteria using nata de coco / nanocomposites TiO2, nata de coco / Ag, and nata de coco / TiO2 / Ag with variations of Ag 0,5 M; 0.6 M; 0.7 M; 0.8 M and 0.9 M using the chemical reduction method. Nanoparticles were characterized using X-Ray Diffractometry (XRD), scanning electron microscopy-energy dispersive X-ray spctroscopy  (SEM-EDS) and anti-bacterial actvity. Test the antibacterial activity using the paper disc method and repeated two times for each sample and bacteria tested. The results showed that the good anti-bacterial activity of nanocomposites in inhibiting bacterial growth was nanocomposite nata de coco /TiO2/Ag  able to inhibit the growth of Escherichia coli and S. aureus, but the nanocomposite is more effective in inhibiting the growth of Escherichia  coli bacteria.

scholarly journals Effect of Gold Nanoparticles Size Capped with Surfactant on the Transformation of Plasmid into Escherichia coli Bacteria

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Amr D ◽  
◽  
Attia N ◽  
Seufi A ◽  
Galal A ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gold Nanoparticles ◽  
Heat Shock ◽  
Transformation Efficiency ◽  
Transformation Process ◽  
Bacterial Cells ◽  
Bacterial Transformation ◽  
Foreign Genes ◽  
Escherichia Coli Bacteria ◽  
Convenient Technique

Bacterial transformation has great importance in molecular biology, as it is used for introduction of foreign genes into bacterial cells either chemical or physical ways. Using calcium chloride to prepare competent cells and heat shock is the most widely used method for bacterial transformation. This method is an efficient and convenient technique but it has in some extent low transformation efficiency. Here we report the use of nanoparticles that significantly improve the transformation efficiency up to 10 times higher than the standard heat shock method by the assistance of (˜ 15, 25 nm) SDS capped gold nanoparticles in the transformation process that leads to the formation of temporary nano-channels across the bacterial cell wall to deliver plasmids into cells. Transformation of bacteria with plasmid was examined using Β-galactosidase assay.

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