High-Yield, Ultrafast, Surface Plasmon-Enhanced, Au Nanorod Optical Field Electron Emitter Arrays

ACS Nano ◽  
2014 ◽  
Vol 8 (11) ◽  
pp. 11474-11482 ◽  
Author(s):  
Richard G. Hobbs ◽  
Yujia Yang ◽  
Arya Fallahi ◽  
Philip D. Keathley ◽  
Eva De Leo ◽  
...  
2013 ◽  
Vol 7 (7) ◽  
pp. 489-492 ◽  
Author(s):  
Debasish Ghosh ◽  
Pradip Ghosh ◽  
Golap Kalita ◽  
Takuto Noda ◽  
Chisato Takahashi ◽  
...  

2006 ◽  
Vol 32 (7) ◽  
pp. 579-581 ◽  
Author(s):  
D. P. Bernatskii ◽  
V. G. Pavlov

2016 ◽  
Vol 41 (10) ◽  
pp. 2310 ◽  
Author(s):  
Wenjun Wu ◽  
Junbo Yang ◽  
Jingjing Zhang ◽  
Jie Huang ◽  
Dingbo Chen ◽  
...  

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5392
Author(s):  
Cecy R Xi ◽  
Arianna Di Fazio ◽  
Naveed Ahmed Nadvi ◽  
Karishma Patel ◽  
Michelle Sui Wen Xiang ◽  
...  

Proteases catalyse irreversible posttranslational modifications that often alter a biological function of the substrate. The protease dipeptidyl peptidase 4 (DPP4) is a pharmacological target in type 2 diabetes therapy primarily because it inactivates glucagon-like protein-1. DPP4 also has roles in steatosis, insulin resistance, cancers and inflammatory and fibrotic diseases. In addition, DPP4 binds to the spike protein of the MERS virus, causing it to be the human cell surface receptor for that virus. DPP4 has been identified as a potential binding target of SARS-CoV-2 spike protein, so this question requires experimental investigation. Understanding protein structure and function requires reliable protocols for production and purification. We developed such strategies for baculovirus generated soluble recombinant human DPP4 (residues 29–766) produced in insect cells. Purification used differential ammonium sulphate precipitation, hydrophobic interaction chromatography, dye affinity chromatography in series with immobilised metal affinity chromatography, and ion-exchange chromatography. The binding affinities of DPP4 to the SARS-CoV-2 full-length spike protein and its receptor-binding domain (RBD) were measured using surface plasmon resonance and ELISA. This optimised DPP4 purification procedure yielded 1 to 1.8 mg of pure fully active soluble DPP4 protein per litre of insect cell culture with specific activity >30 U/mg, indicative of high purity. No specific binding between DPP4 and CoV-2 spike protein was detected by surface plasmon resonance or ELISA. In summary, a procedure for high purity high yield soluble human DPP4 was achieved and used to show that, unlike MERS, SARS-CoV-2 does not bind human DPP4.


2013 ◽  
Vol 811 ◽  
pp. 186-191
Author(s):  
J.C. Juarez-Morales ◽  
Ma. Lourdes Granados-Marin

Associating a perturbation term for the effective refractive index which may depend ontime or position we describe the extremal features for the surface plasmon fields propagating on aroughness metal surface. This representation allows us to interpret the surface optical field as ageodesic flow which in principle enables us to associate coherence features to plasmon modes andto analyze the stability of the surface fields under small perturbations of the refractive index.


2014 ◽  
Vol 6 (13) ◽  
pp. 10469-10475 ◽  
Author(s):  
Jitao Li ◽  
Chunxiang Xu ◽  
Haiyan Nan ◽  
Mingming Jiang ◽  
Guangyu Gao ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document