scholarly journals Mapping Photothermally Induced Gene Expression in Living Cells and Tissues by Nanorod-Locked Nucleic Acid Complexes

ACS Nano ◽  
2014 ◽  
Vol 8 (4) ◽  
pp. 3597-3605 ◽  
Author(s):  
Reza Riahi ◽  
Shue Wang ◽  
Min Long ◽  
Na Li ◽  
Pei-Yu Chiou ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nucleic Acid ◽  
Living Cells ◽  
Locked Nucleic Acid

scholarly journals Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
T. Jarzembowski ◽  
A. Daca ◽  
J. Witkowski ◽  
B. Rutkowski ◽  
J. Gołębiewska ◽  
...  
Keyword(s):  
Gene Expression ◽  
Renal Transplantation ◽  
Nucleic Acid ◽  
Resistance Gene ◽  
Detailed Comparison ◽  
Locked Nucleic Acid ◽  
Planktonic Cells ◽  
Fish Method ◽  
Lna Probe

The aim of the study was to evaluate changes in expression of PBP5 gene associated with immunosuppression. A linear locked nucleic acid (LNA) probe was used to measure resistance gene expression by the Flow-FISH method. Expression of the PBP5 gene measured by Flow-FISH was higher in enterococcal strains isolated from renal transplantation (RTx) recipients than in commensal strains. Additionally, in contrast to commensal strains in isolates from RTx patients, PBP5 gene expression was 17.45% higher in biofilms than in planktonic cells. Detailed comparison also showed that cyclosporine seemed to induce higher expression of PBP5 as compared to tacrolimus.

scholarly journals Detection of mRNA in living cells by double-stranded locked nucleic acid probes

The Analyst ◽  
2013 ◽  
Vol 138 (17) ◽  
pp. 4777 ◽  
Author(s):  
Reza Riahi ◽  
Zachary Dean ◽  
Ting-Hsiang Wu ◽  
Michael A. Teitell ◽  
Pei-Yu Chiou ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Living Cells ◽  
Locked Nucleic Acid ◽  
Nucleic Acid Probes

scholarly journals Liver-Targeted Anti-HBV Single-Stranded Oligonucleotides with Locked Nucleic Acid Potently Reduce HBV Gene Expression In Vivo

2018 ◽  
Vol 11 ◽  
pp. 441-454 ◽  
Author(s):  
Hassan Javanbakht ◽  
Henrik Mueller ◽  
Johanna Walther ◽  
Xue Zhou ◽  
Anaïs Lopez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nucleic Acid ◽  
Locked Nucleic Acid

Hepatitis C virus gene-specific locked nucleic acid enzyme significantly inhibits C gene expression in vitro

2014 ◽  
Vol 22 (14) ◽  
pp. 1992
Author(s):  
Yi-Bin Deng ◽  
Le-Gen Nong ◽  
Zuo-Ren Liang ◽  
Liang Zhang ◽  
Yu-Hua Qin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Nucleic Acid ◽  
Locked Nucleic Acid ◽  
Virus Gene

scholarly journals Exploration of anti EFG1 locked nucleic acid gapmers to control Candida albicans filamentation

2021 ◽  
Vol 3 (12) ◽  
Author(s):  
Daniela Araújo ◽  
Dalila Mil-Homens ◽  
Per Trolle Jørgensen ◽  
Arsénio M. Fialho ◽  
Jesper Wengel ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nucleic Acid ◽  
Chemical Modification ◽  
Therapeutic Potential ◽  
Locked Nucleic Acid ◽  
Novel Approach ◽  
Candida Infections ◽  
In Vitro Performance

Introduction: Antisense oligonucleotides (ASOs) have been successfully utilized to silence gene expression for the treatment of many genetic human diseases, and particularly the locked nucleic acid (LNA) chemical modification is extensively used with this propose. However, LNA-modified ASOs have never been exploited for controlling virulence genes of Candida. EFG1is an important determinant of virulence that is involved in the switch from yeast to filamentous forms in C. albicans. Thus, our main goal was to explore LNA antisense gapmers for controlling EFG1gene expression and to block C. albicans filamentation. Methods: A set of five LNA-modified gapmers were designed with different chemical modifications (phosphorothioate backbone (PS) and/or palmitoyl-2’-amino-LNA) and ASO length. The in vitro performance of the different ASOs was evaluatedon their ability to control EFG1 gene expression, by qRT-PCR, and to reduce C. albicans’ filamentation, through filaments’ enumeration by microscopy. The in vivo therapeutic potential of ASOs was assessed using a G. mellonella model of infection, through a survival assay. Results: In vitro results showed that all ASOs were able to reduce the levels of EFG1gene expression, consequently reducing the levels of C. albicans filamentation around 50%. Interestingly, in vivo tests showed that the LNA-modified gapmer with PS backbone and palmitoyl-2’-amino-LNA was more effective at preventing G. mellonella infections. Conclusions: Undeniably, this work promotes the development of a novel approach for the treatment of Candida infections based on the delivery of ASOs coupled with LNA chemical modification.

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