Photouncaging Nanoparticles for MRI and Fluorescence Imaging in Vitro and in Vivo

ACS Nano ◽  
2013 ◽  
Vol 7 (11) ◽  
pp. 9851-9859 ◽  
Author(s):  
Edakkattuparambil S. Shibu ◽  
Kenji Ono ◽  
Sakiko Sugino ◽  
Ayami Nishioka ◽  
Akikazu Yasuda ◽  
...  
Keyword(s):  
Fluorescence Imaging

scholarly journals In vivo and in vitro tracking of erosion in biodegradable materials using non-invasive fluorescence imaging

2011 ◽  
Vol 10 (9) ◽  
pp. 890-890 ◽  
Author(s):  
Natalie Artzi ◽  
Nuria Oliva ◽  
Cristina Puron ◽  
Sagi Shitreet ◽  
Shay Artzi ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Biodegradable Materials ◽  
Non Invasive

Glycosyl-Modified Diporphyrins for in Vitro and in Vivo Fluorescence Imaging

ChemBioChem ◽  
2013 ◽  
Vol 14 (8) ◽  
pp. 979-986 ◽  
Author(s):  
Ming Wu ◽  
Zuo-Wei Yu ◽  
Yang Liu ◽  
Dao-Fu Feng ◽  
Jia-Jia Yang ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
In Vivo Fluorescence ◽  
In Vivo Fluorescence Imaging

Y2O3:Yb,Er@mSiO2–CuxS double-shelled hollow spheres for enhanced chemo-/photothermal anti-cancer therapy and dual-modal imaging

Nanoscale ◽  
2015 ◽  
Vol 7 (28) ◽  
pp. 12180-12191 ◽  
Author(s):  
Dan Yang ◽  
Guixin Yang ◽  
Xingmei Wang ◽  
Ruichan Lv ◽  
Shili Gai ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Fluorescence Imaging ◽  
Hollow Spheres ◽  
Anti Cancer ◽  
Imaging Properties

Y2O3:Yb,Er@mSiO2 double-shelled hollow spheres (DSHSs) exhibit high anti-cancer efficacy due to enabling synergistic therapy and their excellent in vitro and in vivo CT and up-conversion fluorescence imaging properties.

scholarly journals Erratum: In vivo and in vitro tracking of erosion in biodegradable materials using non-invasive fluorescence imaging

2011 ◽  
Vol 10 (11) ◽  
pp. 896-896 ◽  
Author(s):  
Natalie Artzi ◽  
Nuria Oliva ◽  
Cristina Puron ◽  
Sagi Shitreet ◽  
Shay Artzi ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Biodegradable Materials ◽  
Non Invasive

IN VIVO NEAR-INFRARED FLUORESCENCE IMAGING OF HUMAN COLON ADENOCARCINOMA BY SPECIFIC IMMUNOTARGETING OF A TUMOR-ASSOCIATED MUCIN

2009 ◽  
Vol 02 (04) ◽  
pp. 407-422 ◽  
Author(s):  
RALPH S. DACOSTA ◽  
YING TANG ◽  
TUULA KALLIOMAKI ◽  
RAYMOND M. REILLY ◽  
ROBERT WEERSINK ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescence Imaging ◽  
Normal Tissue ◽  
Near Infrared ◽  
Ex Vivo ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Human Colon Adenocarcinoma

Background and Aims: Accurate endoscopic detection of premalignant lesions and early cancers in the colon is essential for cure, since prognosis is closely related to lesion size and stage. Although it has great clinical potential, autofluorescence endoscopy has limited tumor-to-normal tissue image contrast for detecting small preneoplastic lesions. We have developed a molecularly specific, near-infrared fluorescent monoclonal antibody (CC49) bioconjugate which targets tumor-associated glycoprotein 72 (TAG72), as a contrast agent to improve fluorescence-based endoscopy of colon cancer. Methods: The fluorescent anti-TAG72 conjugate was evaluated in vitro and in vivo in athymic nude mice bearing human colon adenocarcinoma (LS174T) subcutaneous tumors. Autofluorescence, a fluorescent but irrelevant antibody and the free fluorescent dye served as controls. Fluorescent agents were injected intravenously, and in vivo whole body fluorescence imaging was performed at various time points to determine pharmacokinetics, followed by ex vivo tissue analysis by confocal fluorescence microscopy and histology. Results: Fluorescence microscopy and histology confirmed specific LS174T cell membrane targeting of labeled CC49 in vitro and ex vivo. In vivo fluorescence imaging demonstrated significant tumor-to-normal tissue contrast enhancement with labeled-CC49 at three hours post injection, with maximum contrast after 48 h. Accumulation of tumor fluorescence demonstrated that modification of CC49 antibodies did not alter their specific tumor-localizing properties, and was antibody-dependent since controls did not produce detectable tumor fluorescence. Conclusions: These results show proof-of-principle that our near-infrared fluorescent-antibody probe targeting a tumor-associated mucin detects colonic tumors at the molecular level in real time, and offer a basis for future improvement of image contrast during clinical fluorescence endoscopy.

Reverse microemulsion-mediated synthesis of Bi2S3–QD@SiO2–PEG for dual modal CT–fluorescence imaging in vitro and in vivo

2013 ◽  
Vol 49 (100) ◽  
pp. 11800 ◽  
Author(s):  
Jun Chen ◽  
Xiao-Quan Yang ◽  
Yuan-Zheng Meng ◽  
Meng-Yao Qin ◽  
Dong-Mei Yan ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Reverse Microemulsion

scholarly journals Identification on Mantle Cell Lymphoma Using CD20 and CD5 Coupled Upconversion Fluorescent Nanoprobes

2018 ◽  
Vol 2018 ◽  
pp. 1-12
Author(s):  
Mingkai Zhang ◽  
Yang Gao ◽  
Jialiang Wang ◽  
Zhanbo Liu ◽  
Zaishun Jin ◽  
...  
Keyword(s):  
B Cell ◽  
Mantle Cell Lymphoma ◽  
Fluorescence Imaging ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Upconversion Fluorescence ◽  
Mantle Cell

In order to determine a particular tumor cell via nanomaterials, we introduce the preparation of CD20 and CD5 coupled nanoprobes (denoted as CD20 and CD5 nanoprobes for convenience) and an application in identification of mantle cell lymphoma (MCL) from B-cell lymphoma. In this work, CD20 and CD5 nanoprobes were prepared by selectively oxidizing the carbon-carbon double bonds of oleate ligands on the surfaces of NaYF4:Yb3+,Tm3+ and NaYF4:Yb3+,Er3+ nanoparticles and, respectively, coupling carboxyl groups on the particles’ surfaces with CD20 and CD5 monoclonal antibodies through EDC/NHS crosslinking agents. After in situ hybridized Jeko-1 cells and Raji cells as a reference with CD20 and CD5 nanoprobes, in vitro double-color upconversion fluorescence imaging of Jeko-1 cells was demonstrated through visualization of blue and green fluorescence under a 980 nm laser excitation. Moreover, in vivo upconversion fluorescence imaging of the transplanted cancer model was also measured. These experimental results indicate that Jeko-1 cells have been specifically labeled by CD20 and CD5 nanoprobes. It is therefore concluded that CD20 and CD5 nanoprobes could be used to specially differentiate mantle cell lymphoma (MCL) from B-cell lymphoma.

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