scholarly journals In Vitro Toxicity Assessment of Amphiphillic Polymer-Coated CdSe/ZnS Quantum Dots in Two Human Liver Cell Models

ACS Nano ◽  
2012 ◽  
Vol 6 (11) ◽  
pp. 9475-9484 ◽  
Author(s):  
Wesley E. Smith ◽  
Jessica Brownell ◽  
Collin C. White ◽  
Zahra Afsharinejad ◽  
Jesse Tsai ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Liver Cell ◽  
Human Liver ◽  
Toxicity Assessment ◽  
In Vitro Toxicity ◽  
Cell Models ◽  
Human Liver Cell ◽  
In Vitro Toxicity Assessment

Human liver cell cultivation for long-term in vitro toxicity studies in a miniaturized multi-compartment 3D bioreactor system

2011 ◽  
Vol 49 (01) ◽  
Author(s):  
SA Hoffmann ◽  
M Lübberstedt ◽  
U Müller-Vieira ◽  
D Knobeloch ◽  
A Nüssler ◽  
...  
Keyword(s):  
Liver Cell ◽  
Human Liver ◽  
In Vitro Toxicity ◽  
Cell Cultivation ◽  
Toxicity Studies ◽  
Human Liver Cell ◽  
Bioreactor System

scholarly journals Comparing in vitro human liver models to in vivo human liver using RNA-Seq

2020 ◽  
Author(s):  
Rajinder Gupta ◽  
Yannick Schrooders ◽  
Duncan Hauser ◽  
Marcel van Herwijnen ◽  
Wiebke Albrecht ◽  
...  
Keyword(s):  
Liver Cell ◽  
Liver Tissue ◽  
Hepg2 Cells ◽  
Human Liver ◽  
Rna Seq ◽  
Cell Models ◽  
Human Liver Tissue ◽  
Human Liver Cell

Abstract The liver plays an important role in xenobiotic metabolism and represents a primary target for toxic substances. Many different in vitro cell models have been developed in the past decades. In this study, we used RNA-sequencing (RNA-Seq) to analyze the following human in vitro liver cell models in comparison to human liver tissue: cancer-derived cell lines (HepG2, HepaRG 3D), induced pluripotent stem cell-derived hepatocyte-like cells (iPSC-HLCs), cancerous human liver-derived assays (hPCLiS, human precision cut liver slices), non-cancerous human liver-derived assays (PHH, primary human hepatocytes) and 3D liver microtissues. First, using CellNet, we analyzed whether these liver in vitro cell models were indeed classified as liver, based on their baseline expression profile and gene regulatory networks (GRN). More comprehensive analyses using non-differentially expressed genes (non-DEGs) and differential transcript usage (DTU) were applied to assess the coverage for important liver pathways. Through different analyses, we noticed that 3D liver microtissues exhibited a high similarity with in vivo liver, in terms of CellNet (C/T score: 0.98), non-DEGs (10,363) and pathway coverage (highest for 19 out of 20 liver specific pathways shown) at the beginning of the incubation period (0 h) followed by a decrease during long-term incubation for 168 and 336 h. PHH also showed a high degree of similarity with human liver tissue and allowed stable conditions for a short-term cultivation period of 24 h. Using the same metrics, HepG2 cells illustrated the lowest similarity (C/T: 0.51, non-DEGs: 5623, and pathways coverage: least for 7 out of 20) with human liver tissue. The HepG2 are widely used in hepatotoxicity studies, however, due to their lower similarity, they should be used with caution. HepaRG models, iPSC-HLCs, and hPCLiS ranged clearly behind microtissues and PHH but showed higher similarity to human liver tissue than HepG2 cells. In conclusion, this study offers a resource of RNA-Seq data of several biological replicates of human liver cell models in vitro compared to human liver tissue.

Synthesis, characterization and in vitro toxicity assessment of DMPS-capped CdTe quantum dots

Polyhedron ◽  
2014 ◽  
Vol 70 ◽  
pp. 77-84 ◽  
Author(s):  
José S. Casas ◽  
María S. García-Tasende ◽  
Agustín Sánchez ◽  
Ángeles Sánchez-González ◽  
José Sordo ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Toxicity Assessment ◽  
Cdte Quantum Dots ◽  
In Vitro Toxicity ◽  
In Vitro Toxicity Assessment

In vitro kinetics of amiodarone and its major metabolite in two human liver cell models after acute and repeated treatments

2015 ◽  
Vol 30 (1) ◽  
pp. 36-51 ◽  
Author(s):  
Giuliana Pomponio ◽  
Camille C. Savary ◽  
Céline Parmentier ◽  
Frederic Bois ◽  
André Guillouzo ◽  
...  
Keyword(s):  
Liver Cell ◽  
Human Liver ◽  
Major Metabolite ◽  
Cell Models ◽  
Human Liver Cell ◽  
Kinetics Of ◽  
In Vitro Kinetics

In vitro toxicity of 7H-dibenzo[c,g]carbazole in human liver cell lines

2002 ◽  
Vol 16 (3) ◽  
pp. 235-243 ◽  
Author(s):  
T. O'Brien ◽  
J. Schneider ◽  
D. Warshawsky ◽  
K. Mitchell
Keyword(s):  
Cell Lines ◽  
Liver Cell ◽  
Human Liver ◽  
In Vitro Toxicity ◽  
Human Liver Cell

scholarly journals New insight into artifactual phenomena during in vitro toxicity assessment of engineered nanoparticles: Study of TNF-α adsorption on alumina oxide nanoparticle

2013 ◽  
Vol 27 (3) ◽  
pp. 1049-1056 ◽  
Author(s):  
Mélanie Pailleux ◽  
Delphine Boudard ◽  
Jérémie Pourchez ◽  
Valérie Forest ◽  
Philippe Grosseau ◽  
...  
Keyword(s):  
Toxicity Assessment ◽  
Engineered Nanoparticles ◽  
In Vitro Toxicity ◽  
Tnf Α ◽  
Alumina Oxide ◽  
Oxide Nanoparticle ◽  
In Vitro Toxicity Assessment ◽  
Insight Into

scholarly journals In vitro toxicity assessment of emitted materials collected during the manufacture of water pipe plastic linings

2019 ◽  
Vol 31 (4) ◽  
pp. 131-146 ◽  
Author(s):  
Lisa Kobos ◽  
Seyedeh Mahboobeh Teimouri Sendesi ◽  
Andrew J. Whelton ◽  
Brandon E. Boor ◽  
John A. Howarter ◽  
...  
Keyword(s):  
Toxicity Assessment ◽  
In Vitro Toxicity ◽  
Water Pipe ◽  
In Vitro Toxicity Assessment

scholarly journals In vitro toxicity assessment of chitosan oligosaccharide coated iron oxide nanoparticles

2015 ◽  
Vol 2 ◽  
pp. 27-39 ◽  
Author(s):  
Sudeep Shukla ◽  
Alka Jadaun ◽  
Vikas Arora ◽  
Raj Kumar Sinha ◽  
Neha Biyani ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Toxicity Assessment ◽  
In Vitro Toxicity ◽  
Oxide Nanoparticles ◽  
Chitosan Oligosaccharide ◽  
In Vitro Toxicity Assessment
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