scholarly journals Three-Dimensional Orientation Sensors by Defocused Imaging of Gold Nanorods through an Ordinary Wide-Field Microscope

ACS Nano ◽  
2012 ◽  
Vol 6 (2) ◽  
pp. 1268-1277 ◽  
Author(s):  
Tao Li ◽  
Qiang Li ◽  
Yi Xu ◽  
Xiao-Jun Chen ◽  
Qiao-Feng Dai ◽  
...  
Author(s):  
Masamune Oguri ◽  
Satoshi Miyazaki ◽  
Chiaki Hikage ◽  
Rachel Mandelbaum ◽  
Yousuke Utsumi ◽  
...  

Sensors ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 2918 ◽  
Author(s):  
Junseong Eom ◽  
Sangjun Moon

The digital in-line holographic microscope (DIHM) was developed for a 2D imaging technology and has recently been adapted to 3D imaging methods, providing new approaches to obtaining volumetric images with both a high resolution and wide field-of-view (FOV), which allows the physical limitations to be overcome. However, during the sectioning process of 3D image generation, the out-of-focus image of the object becomes a significant impediment to obtaining evident 3D features in the 2D sectioning plane of a thick biological sample. Based on phase retrieved high-resolution holographic imaging and a 3D deconvolution technique, we demonstrate that a high-resolution 3D volumetric image, which significantly reduces wave-front reconstruction and out-of-focus artifacts, can be achieved. The results show a 3D volumetric image that is more finely focused compared to a conventional 3D stacked image from 2D reconstructed images in relation to micron-size polystyrene beads, a whole blood smear, and a kidney tissue sample. We believe that this technology can be applicable for medical-grade images of smeared whole blood or an optically cleared tissue sample for mobile phytological microscopy and laser sectioning microscopy.


2021 ◽  
Author(s):  
Xuepeng Chen ◽  
Weihua Guo ◽  
Jiangcheng Feng ◽  
Yang Su ◽  
Yan Sun ◽  
...  

Abstract Located at a distance of about 300 pc, Perseus OB2 (or Per~OB2 for short) is one of the major OB associations in the solar vicinity\cite{Zeeuw99,Belikov2002}, which has blown a supershell with a diameter of about 15 degree seen in the atomic hydrogen line surveys\cite{Sancisi1974,Heiles1984,Hartmann1997}. It was long considered that stellar feedback from the Per~OB2 association had formed a superbubble that swept up the surrounding interstellar medium into the observed supershell\cite{Bally2008}. Here we report the three-dimensional structure of the Per~OB2 superbubble, based on wide-field atomic hydrogen and molecular gas (traced by CO) surveys. The measured diameter of the superbubble is roughly 330 pc. Multiple atomic hydrogen shells/loops with expansion velocities of about 10 km/s are revealed in the superbubble, suggesting a complicated evolution history of the superbubble. Furthermore, the inspections of the morphology, kinematics and timescale of the Taurus-Auriga, California, and Perseus molecular clouds shows that the cloud complex is a super molecular cloud loop circling around and co-expanding with the Per~OB2 superbubble. We conclude that the Taurus-Auriga-California-Perseus loop, the largest star-forming molecular cloud complex in the solar neighborhood, is formed from the feedback of the Per~OB2 superbubble.


2016 ◽  
Vol 14 (3) ◽  
pp. 031201-31205 ◽  
Author(s):  
Wu Zhou Wu Zhou ◽  
Na Jin Na Jin ◽  
Minhua Jia Minhua Jia ◽  
Huinan Yang Huinan Yang ◽  
and Xiaoshu Cai and Xiaoshu Cai

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 293
Author(s):  
Wenhao Du ◽  
Cheng Fei ◽  
Junliang Liu ◽  
Yongfu Li ◽  
Zhaojun Liu ◽  
...  

Optical projection tomography (OPT) is the direct optical equivalent of X-ray computed tomography (CT). To obtain a larger depth of field, traditional OPT usually decreases the numerical aperture (NA) of the objective lens to decrease the resolution of the image. So, there is a trade-off between sample size and resolution. Commercial microfluidic systems can observe a sample in flow mode. In this paper, an OPT instrument is constructed to observe samples. The OPT instrument is combined with commercial microfluidic systems to obtain a three-dimensional and time (3D + T)/four-dimensional (4D) video of the sample. “Focal plane scanning” is also used to increase the images’ depth of field. A series of two-dimensional (2D) images in different focal planes was observed and compared with images simulated using our program. Our work dynamically monitors 3D OPT images. Commercial microfluidic systems simulate blood flow, which has potential application in blood monitoring and intelligent drug delivery platforms. We design an OPT adaptor to perform OPT on a commercial wide-field inverted microscope (Olympusix81). Images in different focal planes are observed and analyzed. Using a commercial microfluidic system, a video is also acquired to record motion pictures of samples at different flow rates. To our knowledge, this is the first time an OPT setup has been combined with a microfluidic system.


RSC Advances ◽  
2020 ◽  
Vol 10 (50) ◽  
pp. 29868-29872
Author(s):  
Geun Wan Kim ◽  
Seokyoung Yoon ◽  
Jung Heon Lee ◽  
Ji Won Ha

Spherical AuNRs@mSiO2 have randomly oriented AuNR cores in 3D space, which could be resolved on the same focal plane by interference-based DIC microscopy.


2016 ◽  
Vol 24 (13) ◽  
pp. 14124 ◽  
Author(s):  
Guanhua Lv ◽  
Jinxiang Li ◽  
Shao-Long Tie ◽  
Sheng Lan

Sign in / Sign up

Export Citation Format

Share Document