Nanocarrier Cross-Linking Density and pH Sensitivity Regulate Intracellular Gene Transfer

Nano Letters ◽  
2009 ◽  
Vol 9 (12) ◽  
pp. 4467-4473 ◽  
Author(s):  
Jin-Oh You ◽  
Debra T. Auguste
Keyword(s):  
Gene Transfer ◽  
Ph Sensitivity ◽  
Cross Linking ◽  
Intracellular Gene Transfer

scholarly journals Adenovirus vector infection of chronic lymphocytic leukemia B cells

Blood ◽  
1996 ◽  
Vol 88 (12) ◽  
pp. 4676-4683 ◽  
Author(s):  
MJ Cantwell ◽  
S Sharma ◽  
T Friedmann ◽  
TJ Kipps
Keyword(s):  
B Cells ◽  
Chronic Lymphocytic Leukemia ◽  
Gene Transfer ◽  
Hela Cells ◽  
Recombinant Adenovirus ◽  
Lymphocytic Leukemia ◽  
Cross Linking ◽  
Gene Transduction ◽  
Transfer Genes ◽  
Adenovirus Vectors

Adenovirus vectors have several features that make them attractive for potential use in gene therapy, including a broad tissue tropism and an ability to infect quiescent or postmitotic cells. In light of this, we examined whether recombinant adenovirus vectors could transfer genes into neoplastic cells of patients with chronic lymphocytic leukemia (CLL), a leukemia of “resting” B cells. Using high-titer recombinant adenovirus vectors, we found we could transfer genes encoding beta-galactosidase or murine CD80 (B7–1) into the CLL B cells of all patients tested (n = 10). The efficiency of gene transduction into CLL B cells was approximately 100 to 1,000-fold lower than into HeLa cells at any given multiplicity of infection (MOI). At a MOI of 500, 10% to 70% of the CLL B cells from different patients were made to express the transgene, as assessed by multiparameter flow cytometric analysis. Sustained levels of expression with little loss in the percentage of infected cells were maintained for up to 9 days, at which point the analysis was stopped. We found that CLL B cells have markedly lower expression levels of integrins that facilitate internalization of adenovirus particles into target cells, perhaps accounting, in part, for the reduced efficiency of adenovirus-mediated gene transfer compared with that in HeLa cells. Although HeLa cells express high levels of alpha(v)beta5, and detectable amounts of alpha(v)beta3, we find CLL cells from all patients tested express only low amounts of alpha(v)beta3, and no detectable alpha(v)beta5. Activation of CLL cells via CD40 cross-linking enhances expression of alpha(v)beta3, and induces expression of alpha(v)beta5. This phenotypic change is associated with a fivefold increase in the efficiency of adenovirus-mediated gene transfer into such activated CLL B cells. This study demonstrates that adenovirus vectors can transduce genes into CLL B cells and that the efficiency of gene transduction is enhanced by activation via CD40 cross-linking. This is the first demonstration that high proportions of CLL B cells can be made to express a selected transgene, suggesting that such gene transfer methods may become useful for the study of the pathogenesis and/or treatment of this disease.

Chitosan oligosaccharide as prospective cross-linking agent for naproxen-loaded Ca-alginate microparticles with improved pH sensitivity

2012 ◽  
Vol 39 (1) ◽  
pp. 77-88 ◽  
Author(s):  
Bojan Čalija ◽  
Jela Milić ◽  
Nebojša Cekić ◽  
Danina Krajišnik ◽  
Rolf Daniels ◽  
...  
Keyword(s):  
Ph Sensitivity ◽  
Cross Linking ◽  
Chitosan Oligosaccharide ◽  
Ca Alginate

scholarly journals Intracellular gene transfer in action: Dual transcription and multiple silencings of nuclear and mitochondrial cox2 genes in legumes

1999 ◽  
Vol 96 (24) ◽  
pp. 13863-13868 ◽  
Author(s):  
K. L. Adams ◽  
K. Song ◽  
P. G. Roessler ◽  
J. M. Nugent ◽  
J. L. Doyle ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Intracellular Gene Transfer

scholarly journals Complete Plastid and Mitochondrial Genomes of Aeginetia indica Reveal Intracellular Gene Transfer (IGT), Horizontal Gene Transfer (HGT), and Cytoplasmic Male Sterility (CMS)

2021 ◽  
Vol 22 (11) ◽  
pp. 6143
Author(s):  
Kyoung-Su Choi ◽  
Seonjoo Park
Keyword(s):  
Gene Transfer ◽  
Horizontal Gene Transfer ◽  
Chloroplast Genome ◽  
Mitochondrial Genes ◽  
Rrna Genes ◽  
Mitochondrial Genomes ◽  
Reading Frame ◽  
Chloroplast Genomes ◽  
Intracellular Gene Transfer ◽  
Atpi Gene

Orobanchaceae have become a model group for studies on the evolution of parasitic flowering plants, and Aeginetia indica, a holoparasitic plant, is a member of this family. In this study, we assembled the complete chloroplast and mitochondrial genomes of A. indica. The chloroplast and mitochondrial genomes were 56,381 bp and 401,628 bp long, respectively. The chloroplast genome of A. indica shows massive plastid genes and the loss of one IR (inverted repeat). A comparison of the A. indica chloroplast genome sequence with that of a previous study demonstrated that the two chloroplast genomes encode a similar number of proteins (except atpH) but differ greatly in length. The A. indica mitochondrial genome has 53 genes, including 35 protein-coding genes (34 native mitochondrial genes and one chloroplast gene), 15 tRNA (11 native mitochondrial genes and four chloroplast genes) genes, and three rRNA genes. Evidence for intracellular gene transfer (IGT) and horizontal gene transfer (HGT) was obtained for plastid and mitochondrial genomes. ψndhB and ψcemA in the A. indica mitogenome were transferred from the plastid genome of A. indica. The atpH gene in the plastid of A. indica was transferred from another plastid angiosperm plastid and the atpI gene in mitogenome A. indica was transferred from a host plant like Miscanthus siensis. Cox2 (orf43) encodes proteins containing a membrane domain, making ORF (Open Reading Frame) the most likely candidate gene for CMS development in A. indica.

Agar/PAAc-Fe3+ hydrogels with pH-sensitivity and high toughness using dual physical cross-linking

2018 ◽  
Vol 27 (11) ◽  
pp. 829-840 ◽  
Author(s):  
Xuefeng Li ◽  
Yikun Zhang ◽  
Qian Yang ◽  
Dapeng Li ◽  
Gaowen Zhang ◽  
...  
Keyword(s):  
Ph Sensitivity ◽  
Cross Linking ◽  
High Toughness

scholarly journals Characterization and Dynamics of Intracellular Gene Transfer in Plastid Genomes of Viola (Violaceae) and Order Malpighiales

2021 ◽  
Vol 12 ◽  
Author(s):  
JiYoung Yang ◽  
Seongjun Park ◽  
Hee-Young Gil ◽  
Jae-Hong Pak ◽  
Seung-Chul Kim
Keyword(s):  
Gene Transfer ◽  
Common Ancestor ◽  
Transit Peptide ◽  
Island Endemic ◽  
Plastid Genomes ◽  
Alternative Mrna Splicing ◽  
Intracellular Gene Transfer ◽  
Phylogenetic Framework ◽  
The Common ◽  
Functional Replacement

Functional gene transfer from organelles to the nucleus, known as intracellular gene transfer (IGT), is an ongoing process in flowering plants. The complete plastid genomes (plastomes) of two Ulleung island endemic violets, Viola ulleungdoensis and V. woosanensis, were characterized, revealing a lack of the plastid-encoded infA, rpl32, and rps16 genes. In addition, functional replacement of the three plastid-encoded genes in the nucleus was confirmed within the genus Viola and the order Malpighiales. Three strategies for the acquisition of a novel transit peptide for successful IGT were identified in the genus Viola. Nuclear INFA acquired a novel transit peptide with very low identity between these proteins, whereas the nuclear RPL32 gene acquired an existing transit peptide via fusion with the nuclear-encoded plastid-targeted SOD gene (Cu-Zn superoxide dismutase superfamily) as one exon, and translated both proteins in the cytosol using alternative mRNA splicing. Nuclear RPS16 contains an internal transit peptide without an N-terminal extension. Gene loss or pseudogenization of the plastid-borne rpl32 and rps16 loci was inferred to occur in the common ancestor of the genus Viola based on an infrageneric phylogenetic framework in Korea. Although infA was lost in the common ancestor of the order Malpighiales, the rpl32 and rps16 genes were lost multiple times independently within the order. Our current study sheds additional light on plastid genome composition and IGT mechanisms in the violet genus and in the order Malpighiales.

Photoinduced Cross-Linking of Star Vector for Improvement of Gene Transfer Efficiency

2008 ◽  
Vol 19 (12) ◽  
pp. 2513-2519 ◽  
Author(s):  
Yasushi Nemoto ◽  
Yue-Min Zhou ◽  
Eisuke Tatsumi ◽  
Yasuhide Nakayama
Keyword(s):  
Gene Transfer ◽  
Transfer Efficiency ◽  
Cross Linking ◽  
Gene Transfer Efficiency
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