FluidFM: Combining Atomic Force Microscopy and Nanofluidics in a Universal Liquid Delivery System for Single Cell Applications and Beyond

Nano Letters ◽  
2009 ◽  
Vol 9 (6) ◽  
pp. 2501-2507 ◽  
Author(s):  
André Meister ◽  
Michael Gabi ◽  
Pascal Behr ◽  
Philipp Studer ◽  
János Vörös ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Single Cell ◽  
Delivery System ◽  
Force Microscopy ◽  
Atomic Force ◽  
Liquid Delivery

Visualization and Quantification of MicroRNA in a Single Cell Using Atomic Force Microscopy

2016 ◽  
Vol 138 (36) ◽  
pp. 11664-11671 ◽  
Author(s):  
Hyunseo Koo ◽  
Ikbum Park ◽  
Yoonhee Lee ◽  
Hyun Jin Kim ◽  
Jung Hoon Jung ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Single Cell ◽  
Force Microscopy ◽  
Atomic Force

Living cell study at the single-molecule and single-cell levels by atomic force microscopy

Nanomedicine ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. 1625-1637 ◽  
Author(s):  
Xiaoli Shi ◽  
Xuejie Zhang ◽  
Tie Xia ◽  
Xiaohong Fang
Keyword(s):  
Atomic Force Microscopy ◽  
Single Cell ◽  
Single Molecule ◽  
Living Cell ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cell Study

scholarly journals Chondrocyte cell adhesion on chitosan supports using single-cell atomic force microscopy

2021 ◽  
pp. 1-15
Author(s):  
Christian Enrique García García ◽  
Claude Verdier ◽  
Bernard Lardy ◽  
Frédéric Bossard ◽  
J. Félix Armando Soltero Martínez ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Adhesion ◽  
Single Cell ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Extending applications of AFM to fluidic AFM in single living cell and extracellular vesicle studies

2021 ◽  
Author(s):  
Yuan Qiu ◽  
Chen-Chi Chien ◽  
Basilis Maroulis ◽  
Angelo Gaitas ◽  
Bin Gong
Keyword(s):  
Atomic Force Microscopy ◽  
Single Cell ◽  
Living Cell ◽  
Cell Monolayer ◽  
Extracellular Vesicle ◽  
Liquid Environment ◽  
Force Microscopy ◽  
Atomic Force ◽  
Single Living Cell ◽  
Single Living

Abstract In this article, a review of the application of atomic force microscopy (AFM) for the analyses of extracellular vesicles is presented. This information is then extended to include fluidic Atomic Force Microscopy (fluidic AFM) applications. Fluidic AFM is an offshoot of AFM that combines a microfluidic cantilever with AFM and has enabled the research community to conduct biological, pathological, and pharmacological studies on cells at the single-cell level in a liquid environment. AFM applications involving single cell and extracellular vesicle studies, colloidal force spectroscopy, and single cell adhesion measurements are discussed. In this review, new results are offered, using fluidic AFM, to illustrate (1) the speed with which sequential measurements of adhesion using coated colloid beads can be done, (2) the ability to assess lateral binding forces (LBFs) of endothelial or epithelial cells in a confluent cell monolayer in appropriate physiological environment, and (3) the ease of measurement of vertical binding force (VBFs) of intercellular adhesion between heterogeneous cells. Finally, key applications are discussed that include extracellular vesicle absorption, manipulation of a single living cell by intracellular injection, sampling of cellular fluid from a single living cell, patch clamping, and mass measurements of a single living cell.

High-throughput Measurements of Single Cell Rheology by Atomic Force Microscopy

2015 ◽  
pp. 57-67
Author(s):  
Kaori Kuribayashi-Shigetomi ◽  
Ryosuke Takahashi ◽  
Agus Subagyo ◽  
Kazuhisa Sueoka ◽  
Takaharu Okajima
Keyword(s):  
Atomic Force Microscopy ◽  
Single Cell ◽  
High Throughput ◽  
Force Microscopy ◽  
Cell Rheology ◽  
Atomic Force

scholarly journals A multi-structural finite element model to simulate atomic force microscopy nanoindentation of single cells

2019 ◽  
Author(s):  
Stefania Marcotti ◽  
Gwendolen C Reilly ◽  
Damien Lacroix
Keyword(s):  
Atomic Force Microscopy ◽  
Finite Element ◽  
Single Cell ◽  
Cell Nucleus ◽  
Single Cells ◽  
Element Model ◽  
Cell Stiffness ◽  
Force Microscopy ◽  
Atomic Force ◽  
Experimental Findings

AbstractSingle cell mechanical properties represent an increasingly studied descriptor for health and disease. Atomic force microscopy (AFM) has been widely used to measure single cell stiffness, despite its experimental limitations. The development of a computational framework to simulate AFM nanoindentation experiments could be a valuable tool to complement experimental findings. A single cell multi-structural finite element model was designed to this aim by using confocal images of bone cells, comprised of the cell nucleus, cytoplasm and actin cytoskeleton. The computational cell stiffness values were in the range of experimental values acquired on the same cells for nanoindentation of the cell nucleus and periphery, despite showing higher stiffness for the nucleus than for the periphery, oppositely to the average experimental findings. These results suggest it would be of interest to model different single cells with known experimental effective moduli to evaluate the ability of the computational models to replicate experimental results.

Atomic force microscopy-based bioanalysis for the study of disease

2014 ◽  
Vol 6 (14) ◽  
pp. 4932-4955 ◽  
Author(s):  
Kirstin C. Morton ◽  
Lane A. Baker
Keyword(s):  
Atomic Force Microscopy ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Subject Area ◽  
Cell Analysis ◽  
Force Microscopy ◽  
Disease States ◽  
Atomic Force ◽  
Recent Advances

This review aims to describe the operation of atomic force microscopy related to the study of disease states and single cell analysis, and to serve as an overview of recent advances in this subject area.

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