scholarly journals High-Speed Single-Particle Tracking of GM1 in Model Membranes Reveals Anomalous Diffusion due to Interleaflet Coupling and Molecular Pinning

Nano Letters ◽  
2014 ◽  
Vol 14 (9) ◽  
pp. 5390-5397 ◽  
Author(s):  
Katelyn M. Spillane ◽  
Jaime Ortega-Arroyo ◽  
Gabrielle de Wit ◽  
Christian Eggeling ◽  
Helge Ewers ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Anomalous Diffusion ◽  
Single Particle ◽  
High Speed ◽  
Single Particle Tracking ◽  
Model Membranes

scholarly journals High-Density Single Particle Tracking on the Plasma Membrane using High-Speed Hyperspectral Imaging

2012 ◽  
Vol 102 (3) ◽  
pp. 581a
Author(s):  
Patrick J. Cutler ◽  
Michael D. Malik ◽  
Sheng Liu ◽  
Jason M. Byars ◽  
Diane S. Lidke ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Hyperspectral Imaging ◽  
Particle Tracking ◽  
Single Particle ◽  
High Speed ◽  
Single Particle Tracking ◽  
High Density

scholarly journals Organization of the axon initial segment: Actin like a fence

2016 ◽  
Vol 215 (1) ◽  
pp. 9-11 ◽  
Author(s):  
Yu-Mei Huang ◽  
Matthew N. Rasband
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
High Speed ◽  
Initial Segment ◽  
Single Particle Tracking ◽  
Axon Initial Segment ◽  
Membrane Domains ◽  
Superresolution Microscopy ◽  
Cell Biol ◽  
Actin Rings

What prevents the movement of membrane molecules between axonal and somatodendritic domains is unclear. In this issue, Albrecht et. al. (2016. J. Cell Biol. http://dx.doi.org/10.1083/jcb.201603108) demonstrate via high-speed single-particle tracking and superresolution microscopy that lipid-anchored molecules in the axon initial segment are confined to membrane domains separated by periodically spaced actin rings.

scholarly journals Small stepping motion of processive dynein revealed by load-free high-speed single-particle tracking

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jun Ando ◽  
Tomohiro Shima ◽  
Riko Kanazawa ◽  
Rieko Shimo-Kon ◽  
Akihiko Nakamura ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
High Speed ◽  
Single Particle Tracking

scholarly journals Anomalous diffusion models and their properties: non-stationarity, non-ergodicity, and ageing at the centenary of single particle tracking

2014 ◽  
Vol 16 (44) ◽  
pp. 24128-24164 ◽  
Author(s):  
Ralf Metzler ◽  
Jae-Hyung Jeon ◽  
Andrey G. Cherstvy ◽  
Eli Barkai
Keyword(s):  
Particle Tracking ◽  
Anomalous Diffusion ◽  
Single Particle ◽  
Diffusion Processes ◽  
Diffusion Models ◽  
Single Particle Tracking ◽  
Diffusion Data

This Perspective summarises the properties of a variety of anomalous diffusion processes and provides the necessary tools to analyse and interpret recorded anomalous diffusion data.

scholarly journals Probing the type of anomalous diffusion with single-particle tracking

2014 ◽  
Vol 16 (17) ◽  
pp. 7686-7691 ◽  
Author(s):  
Dominique Ernst ◽  
Jürgen Köhler ◽  
Matthias Weiss
Keyword(s):  
Random Walk ◽  
Particle Tracking ◽  
Anomalous Diffusion ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Tracking Data

We introduce a versatile method to extract the type of (transient) anomalous random walk from experimental single-particle tracking data.

scholarly journals Nanoscopic compartmentalization of membrane protein motion at the axon initial segment

2016 ◽  
Author(s):  
David Albrecht ◽  
Christian M. Winterflood ◽  
Thomas Tschager ◽  
Helge Ewers
Keyword(s):  
Membrane Protein ◽  
Particle Tracking ◽  
Single Particle ◽  
Diffusion Barrier ◽  
High Speed ◽  
Initial Segment ◽  
Single Particle Tracking ◽  
Axon Initial Segment ◽  
Periodic Pattern ◽  
Protein Motion

AbstractThe axon initial segment (AIS) is enriched in specific adaptor, cytoskeletal and transmembrane molecules. During AIS establishment, a membrane diffusion barrier is formed between the axon and the somatodendritic domain. Recently, an axonal periodic pattern of actin, spectrin and ankyrin forming 190 nm distanced, ring-like structures has been discovered. However, whether this structure is related to the diffusion barrier function is unclear.Here, we performed single particle tracking timecourse experiments on hippocampal neurons during AIS development. We analyzed the mobility of lipid-anchored molecules by high-speed single particle tracking and correlated positions of membrane molecules with the nanoscopic organization of the AIS cytoskeleton.We observe a strong reduction in mobility early in AIS development. Membrane protein motion in the AIS plasma membrane is confined to a repetitive pattern of ~190 nm spaced segments along the AIS axis as early as DIV4 and this pattern alternates with actin rings. Our data provide a new model for the mechanism of the AIS diffusion barrier.

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