Parallel Nanometric 3D Tracking of Intracellular Gold Nanorods Using Multifocal Two-Photon Microscopy

Nano Letters ◽  
2013 ◽  
Vol 13 (3) ◽  
pp. 980-986 ◽  
Author(s):  
Bram van den Broek ◽  
Brian Ashcroft ◽  
Tjerk H. Oosterkamp ◽  
John van Noort
Keyword(s):  
Gold Nanorods ◽  
3D Tracking ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals A Multifocal Two-Photon Microscopy Setup for Parallel 3D Tracking of Gold Nanorods

2010 ◽  
Vol 98 (3) ◽  
pp. 178a ◽  
Author(s):  
Bram van den Broek ◽  
Tjerk H. Oosterkamp ◽  
John van Noort
Keyword(s):  
Gold Nanorods ◽  
3D Tracking ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Recording neural activity in unrestrained animals with 3D tracking two photon microscopy

2017 ◽  
Author(s):  
Doycho Karagyozov ◽  
Mirna Mihovilovic Skanata ◽  
Amanda Lesar ◽  
Marc Gershow
Keyword(s):  
Neural Activity ◽  
Stimulus Presentation ◽  
Fruit Fly ◽  
Excitation Power ◽  
Motion Artifacts ◽  
3D Tracking ◽  
Mechanical Coupling ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
High Bandwidth

Optical recordings of neural activity in behaving animals can reveal the neural correlates of decision making, but such recordings are compromised by brain motion that often accompanies behavior. Two-photon point scanning microscopy is especially sensitive to motion artifacts, and to date, two-photon recording of activity has required rigid mechanical coupling between the brain and microscope. To overcome these difficulties, we developed a two-photon tracking microscope with extremely low latency (360 μs) feedback implemented in hardware. We maintained continuous focus on neurons moving with velocities of 3 mm/s and accelerations of 1 m/s2 both in-plane and axially, allowing high-bandwidth measurements with modest excitation power. We recorded from motor- and inter-neurons in unrestrained freely behaving fruit fly larvae, correlating neural activity with stimulus presentation and behavioral outputs. Our technique can be extended to stabilize recordings in a variety of moving substrates.

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Adaptive Optical Two-Photon Microscopy for Surface-Profiled Living Biological Specimens

ACS Omega ◽  
2020 ◽  
Author(s):  
Kazushi Yamaguchi ◽  
Kohei Otomo ◽  
Yuichi Kozawa ◽  
Motosuke Tsutsumi ◽  
Tomoko Inose ◽  
...  
Keyword(s):  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Publisher Correction: Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging

2021 ◽  
Vol 18 (2) ◽  
pp. 220-220
Author(s):  
Weijian Zong ◽  
Runlong Wu ◽  
Shiyuan Chen ◽  
Junjie Wu ◽  
Hanbin Wang ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Field Of View ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Publisher Correction: Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Vincent D. Ching-Roa ◽  
Eben M. Olson ◽  
Sherrif F. Ibrahim ◽  
Richard Torres ◽  
Michael G. Giacomelli
Keyword(s):  
Dynamic Range ◽  
High Dynamic Range ◽  
Silicon Photomultipliers ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
High Dynamic

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

scholarly journals Two-photon microscopy with simultaneous standard and extended depth of field using a tunable acoustic gradient-index lens

2009 ◽  
Vol 34 (11) ◽  
pp. 1684 ◽  
Author(s):  
Nicolas Olivier ◽  
Alexandre Mermillod-Blondin ◽  
Craig B. Arnold ◽  
Emmanuel Beaurepaire
Keyword(s):  
Depth Of Field ◽  
Gradient Index ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Gradient Index Lens ◽  
Extended Depth Of Field
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