Proteolytic Activity at Quantum Dot-Conjugates: Kinetic Analysis Reveals Enhanced Enzyme Activity and Localized Interfacial “Hopping”

Nano Letters ◽  
2012 ◽  
Vol 12 (7) ◽  
pp. 3793-3802 ◽  
Author(s):  
W. Russ Algar ◽  
Anthony Malonoski ◽  
Jeffrey R. Deschamps ◽  
Juan B. Blanco-Canosa ◽  
Kimihiro Susumu ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Quantum Dot ◽  
Kinetic Analysis ◽  
Proteolytic Activity

scholarly journals SELECTED PROTEOLYTIC ACTIVITY BY EXTRACTS OF DERMESTID LARVAE

2011 ◽  
Vol 19 (19) ◽  
pp. 79-83
Author(s):  
Lavinel G. IONESCU
Keyword(s):  
Enzyme Activity ◽  
Ammonium Sulfate ◽  
Proteolytic Activity ◽  
Specific Activity ◽  
Proteolytic Enzymes ◽  
High Specific Activity ◽  
Water Extract ◽  
Dermestes Maculatus ◽  
Crude Preparation

The larvae of the Beetle Dermestes maculatus De Geer can subsist on a diet consisting largely of protein. Studies have been undertaken to investigate the nature of proteolytic enzymes. A water extract of the larvae yielded a crude preparation that hydrolyzes gelatin, bide powder, hemoglobin substrate, benzoyl-DL-arginine p-nitroamilide, and glutaryl-L-phenylalanine p-nitroanilide. Enzyme activity was found in a non-dialyzable, heat- and acid0labile portion of the extract yielded two fractions with high specific activity towards gelatin. These are precipitated between 40% to 60% saturation of ammonium sulfate and 60% to 80% saturation. The higher specific activity was observed in the 40%-60% fraction. These results suggest that the larvae of these dermestids contain proteolytic enzymes with actions similar to mammalian trypsin and chymotrypsin. The results also suggest that other proteolytic enzymes may be present as well.

Detecting Kallikrein Proteolytic Activity with Peptide-Quantum Dot Nanosensors

2014 ◽  
Vol 6 (14) ◽  
pp. 11529-11535 ◽  
Author(s):  
Joyce C. Breger ◽  
Kim E. Sapsford ◽  
Jessica Ganek ◽  
Kimihiro Susumu ◽  
Michael H. Stewart ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Proteolytic Activity

Proteolytic activity monitored by fluorescence resonance energy transfer through quantum-dot–peptide conjugates

2006 ◽  
Vol 5 (7) ◽  
pp. 581-589 ◽  
Author(s):  
Igor L. Medintz ◽  
Aaron R. Clapp ◽  
Florence M. Brunel ◽  
Theresa Tiefenbrunn ◽  
H. Tetsuo Uyeda ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Fluorescence Resonance Energy Transfer ◽  
Proteolytic Activity ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Peptide Conjugates ◽  
Fluorescence Resonance

Quantum dot-based nanosensors for diagnosis via enzyme activity measurement

2013 ◽  
Vol 13 (4) ◽  
pp. 367-375 ◽  
Author(s):  
Birgitta R Knudsen ◽  
Morten Leth Jepsen ◽  
Yi-Ping Ho
Keyword(s):  
Enzyme Activity ◽  
Quantum Dot ◽  
Activity Measurement

scholarly journals Uremic plasma contains factors inhibiting 1 alpha-hydroxylase activity.

1992 ◽  
Vol 3 (4) ◽  
pp. 947-952
Author(s):  
C H Hsu ◽  
S Patel
Keyword(s):  
Enzyme Activity ◽  
Kinetic Analysis ◽  
Normal Saline ◽  
Saline Solution ◽  
Uremic Toxin ◽  
Enzyme Kinetic ◽  
Hydroxylase Activity ◽  
Normal Plasma ◽  
Guanidinosuccinic Acid ◽  
Plasma Ultrafiltrate

The effect of uremic plasma ultrafiltrate on calcitriol synthesis was investigated. Renal 1 alpha-hydroxylase activity was measured in normal rats infused for 20 h with 20 mL of normal or uremic plasma ultrafiltrate. Renal 1 alpha-hydroxylase activity was determined by the generation of calcitriol measured 5, 10, 20, and 30 min after the reaction was initiated by the addition of cold 25(OH)D3. The activity was significantly lower in rats infused with uremic plasma ultrafiltrate. Kidney homogenates preincubated for 3 h with uremic plasma ultrafiltrate also had significantly lower renal 1 alpha-hydroxylase activity than did those preincubated with normal plasma ultrafiltrate. In addition, the effect of the putative uremic toxin, guanidinosuccinic acid (GSA), on renal 1 alpha-hydroxylase activity was studied. Normal rats infused for 20 h with 20 mL of saline solution containing 1.5 mg/dL of GSA had significantly lower renal 1 alpha-hydroxylase activity than did rats infused with normal saline. The enzyme activity was also lower in kidney homogenates preincubated for 3 h with 4 mg/dL of GSA. Enzyme kinetic analysis revealed that the inhibition of renal 1 alpha-hydroxylase by GSA was noncompetitive. It was concluded that uremic plasma contains substances that directly inhibit renal 1 alpha-hydroxylase activity.

Kinetic analysis of human metallothionein and CdTe quantum dot complexes using fluorescence and voltammetry techniques

2017 ◽  
Vol 160 ◽  
pp. 381-389 ◽  
Author(s):  
Ewelina Guszpit ◽  
Ludmila Krejčová ◽  
Soňa Křížková ◽  
Marta Kepinska ◽  
Lukáš Richtera ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Kinetic Analysis ◽  
Cdte Quantum Dot
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