scholarly journals Three-Dimensional High-Resolution Imaging of Gold Nanorods Uptake in Sentinel Lymph Nodes

Nano Letters ◽  
2011 ◽  
Vol 11 (7) ◽  
pp. 2938-2943 ◽  
Author(s):  
Yeongri Jung ◽  
Roberto Reif ◽  
Yaguang Zeng ◽  
Ruikang K. Wang
Keyword(s):  
High Resolution ◽  
Lymph Nodes ◽  
Gold Nanorods ◽  
Three Dimensional ◽  
Sentinel Lymph Nodes ◽  
High Resolution Imaging ◽  
Resolution Imaging

scholarly journals Plastic embedding immunolabeled large-volume samples for three-dimensional high-resolution imaging

2017 ◽  
Vol 8 (8) ◽  
pp. 3583 ◽  
Author(s):  
Yadong Gang ◽  
Xiuli Liu ◽  
Xiaojun Wang ◽  
Qi Zhang ◽  
Hongfu Zhou ◽  
...  
Keyword(s):  
High Resolution ◽  
Large Volume ◽  
Three Dimensional ◽  
High Resolution Imaging ◽  
Plastic Embedding ◽  
Resolution Imaging

In Vivo Three-Dimensional High-Resolution Imaging of Rodent Retina with Spectral-Domain Optical Coherence Tomography

2007 ◽  
Vol 48 (4) ◽  
pp. 1808 ◽  
Author(s):  
Marco Ruggeri ◽  
Hassan Wehbe ◽  
Shuliang Jiao ◽  
Giovanni Gregori ◽  
Maria E. Jockovich ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
High Resolution ◽  
Three Dimensional ◽  
Spectral Domain ◽  
High Resolution Imaging ◽  
Optical Coherence ◽  
Resolution Imaging

scholarly journals Three-dimensional imaging of mitochondrial cristae complexity using cryo-soft X-ray tomography

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Carla C. Polo ◽  
Miriam H. Fonseca-Alaniz ◽  
Jian-Hua Chen ◽  
Axel Ekman ◽  
Gerry McDermott ◽  
...  
Keyword(s):  
High Resolution ◽  
Three Dimensional ◽  
Linear Attenuation Coefficient ◽  
High Resolution Imaging ◽  
Mitochondrial Matrix ◽  
Morphological Parameter ◽  
Mitochondrial Membranes ◽  
X Ray ◽  
Resolution Imaging ◽  
Neuronal Disorders

AbstractMitochondria are dynamic organelles that change morphology to adapt to cellular energetic demands under both physiological and stress conditions. Cardiomyopathies and neuronal disorders are associated with structure-related dysfunction in mitochondria, but three-dimensional characterizations of the organelles are still lacking. In this study, we combined high-resolution imaging and 3D electron density information provided by cryo-soft X-ray tomography to characterize mitochondria cristae morphology isolated from murine. Using the linear attenuation coefficient, the mitochondria were identified (0.247 ± 0.04 µm−1) presenting average dimensions of 0.90 ± 0.20 µm in length and 0.63 ± 0.12 µm in width. The internal mitochondria structure was successfully identified by reaching up the limit of spatial resolution of 35 nm. The internal mitochondrial membranes invagination (cristae) complexity was calculated by the mitochondrial complexity index (MCI) providing quantitative and morphological information of mitochondria larger than 0.90 mm in length. The segmentation to visualize the cristae invaginations into the mitochondrial matrix was possible in mitochondria with MCI ≥ 7. Altogether, we demonstrated that the MCI is a valuable quantitative morphological parameter to evaluate cristae modelling and can be applied to compare healthy and disease state associated to mitochondria morphology.

scholarly journals Nano-laminography for three-dimensional high-resolution imaging of flat specimens

2013 ◽  
Vol 8 (05) ◽  
pp. C05006-C05006 ◽  
Author(s):  
L Helfen ◽  
F Xu ◽  
H Suhonen ◽  
L Urbanelli ◽  
P Cloetens ◽  
...  
Keyword(s):  
High Resolution ◽  
Three Dimensional ◽  
High Resolution Imaging ◽  
Resolution Imaging
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