High Sensitivity of In Vivo Detection of Gold Nanorods Using a Laser Optoacoustic Imaging System

Nano Letters ◽  
2007 ◽  
Vol 7 (7) ◽  
pp. 1914-1918 ◽  
Author(s):  
Mohammad Eghtedari ◽  
Alexander Oraevsky ◽  
John A. Copland ◽  
Nicholas A. Kotov ◽  
Andre Conjusteau ◽  
...  
Keyword(s):  
Gold Nanorods ◽  
Imaging System ◽  
High Sensitivity ◽  
Optoacoustic Imaging ◽  
In Vivo Detection

scholarly journals A polarized hyperspectral imaging system for in vivo detection: Multiple applications in sunflower leaf analysis

2019 ◽  
Vol 158 ◽  
pp. 258-270 ◽  
Author(s):  
Jun-Li Xu ◽  
Alexia Gobrecht ◽  
Daphné Héran ◽  
Nathalie Gorretta ◽  
Marie Coque ◽  
...  
Keyword(s):  
Hyperspectral Imaging ◽  
Imaging System ◽  
Leaf Analysis ◽  
In Vivo Detection

scholarly journals Recent Advances in the Detection of Neurotransmitters

Chemosensors ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 1 ◽  
Author(s):  
Bo Si ◽  
Edward Song
Keyword(s):  
Ex Vivo ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Mental Illnesses ◽  
Electrochemical Sensing ◽  
Fast Scan Cyclic Voltammetry ◽  
In Vivo Detection ◽  
Recent Developments ◽  
Materials Used

Neurotransmitters are chemicals that act as messengers in the synaptic transmission process. They are essential for human health and any imbalance in their activities can cause serious mental disorders such as Parkinson’s disease, schizophrenia, and Alzheimer’s disease. Hence, monitoring the concentrations of various neurotransmitters is of great importance in studying and diagnosing such mental illnesses. Recently, many researchers have explored the use of unique materials for developing biosensors for both in vivo and ex vivo neurotransmitter detection. A combination of nanomaterials, polymers, and biomolecules were incorporated to implement such sensor devices. For in vivo detection, electrochemical sensing has been commonly applied, with fast-scan cyclic voltammetry being the most promising technique to date, due to the advantages such as easy miniaturization, simple device architecture, and high sensitivity. However, the main challenges for in vivo electrochemical neurotransmitter sensors are limited target selectivity, large background signal and noise, and device fouling and degradation over time. Therefore, achieving simultaneous detection of multiple neurotransmitters in real time with long-term stability remains the focus of research. The purpose of this review paper is to summarize the recently developed sensing techniques with the focus on neurotransmitters as the target analyte, and to discuss the outlook of simultaneous detection of multiple neurotransmitter species. This paper is organized as follows: firstly, the common materials used for developing neurotransmitter sensors are discussed. Secondly, several sensor surface modification approaches to enhance sensing performance are reviewed. Finally, we discuss recent developments in the simultaneous detection capability of multiple neurotransmitters.

scholarly journals X-ray-Fluorescence Imaging for In Vivo Detection of Gold-Nanoparticle-Labeled Immune Cells: A GEANT4 Based Feasibility Study

Cancers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 5759
Author(s):  
Arthur Ungerer ◽  
Theresa Staufer ◽  
Oliver Schmutzler ◽  
Christian Körnig ◽  
Kai Rothkamm ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Cell Tracking ◽  
Immune Cell ◽  
High Sensitivity ◽  
Diagnostic Tools ◽  
Monte Carlo Simulation Study ◽  
X Ray ◽  
In Vivo Detection ◽  
Technological Developments

The growing field of cellular therapies in regenerative medicine and oncology calls for more refined diagnostic tools that are able to investigate and monitor the function and success of said therapies. X-ray Fluorescence Imaging (XFI) can be applied for molecular imaging with nanoparticles, such as gold nanoparticles (GNPs), which can be used in immune cell tracking. We present a Monte Carlo simulation study on the sensitivity of detection and associated radiation dose estimations in an idealized setup of XFI in human-sized objects. Our findings demonstrate the practicability of XFI in human-sized objects, as immune cell tracking with a minimum detection limit of 4.4 × 105 cells or 0.86 μg gold in a cubic volume of 1.78 mm3 can be achieved. Therefore, our results show that the current technological developments form a good basis for high sensitivity XFI.

scholarly journals Real-Time In Vivo Detection and Monitoring of Bacterial Infection Based on NIR-II Imaging

2021 ◽  
Vol 9 ◽  
Author(s):  
Sijia Feng ◽  
Huizhu Li ◽  
Chang Liu ◽  
Mo Chen ◽  
Huaixuan Sheng ◽  
...  
Keyword(s):  
Bacterial Infection ◽  
Real Time ◽  
Bacterial Infections ◽  
Near Infrared ◽  
Imaging System ◽  
Bacterial Load ◽  
Dynamic Imaging ◽  
In Vivo Detection

Treatment according to the dynamic changes of bacterial load in vivo is critical for preventing progression of bacterial infections. Here, we present a lead sulfide quantum dots (PbS QDs) based second near-infrared (NIR-II) fluorescence imaging strategy for bacteria detection and real-time in vivo monitoring. Four strains of bacteria were labeled with synthesized PbS QDs which showed high bacteria labeling efficiency in vitro. Then bacteria at different concentrations were injected subcutaneously on the back of male nude mice for in vivo imaging. A series of NIR-II images taken at a predetermined time manner demonstrated changing patterns of photoluminescence (PL) intensity of infected sites, dynamically imaging a changing bacterial load in real-time. A detection limit around 102–104 CFU/ml was also achieved in vivo. Furthermore, analysis of pathology of infected sites were performed, which showed high biocompatibility of PbS QDs. Therefore, under the guidance of our developed NIR-II imaging system, real-time detection and spatiotemporal monitoring of bacterial infection in vivo can be achieved, thus facilitating anti-infection treatment under the guidance of the dynamic imaging of bacterial load in future.

Hyperlipidemic mice as a model for a real-time in vivo detection of atherosclerosis by gold nanorods-based diffusion reflection technique

2018 ◽  
Vol 12 (1) ◽  
pp. e201800218 ◽  
Author(s):  
Ruchira Chakraborty ◽  
Rinat Ankri ◽  
Dorit Leshem-Lev ◽  
Edith Hochhauser ◽  
Ran Kornowski ◽  
...  
Keyword(s):  
Real Time ◽  
Gold Nanorods ◽  
In Vivo Detection ◽  
Diffusion Reflection

Abstract 5372: In Vivo Myocardial Distribution of Adult Stem Cells Immediately Following Intracoronary Delivery in a Swine Model of Myocardial Infarction

Circulation ◽  
2008 ◽  
Vol 118 (suppl_18) ◽  
Author(s):  
Hung Ly ◽  
Kozo Hoshino ◽  
Irina Pomerantseva ◽  
Yoshiaki Kawase ◽  
Ryuichi Yoneyama ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Adult Stem Cells ◽  
Near Infrared ◽  
Imaging System ◽  
High Sensitivity ◽  
Cell Loss ◽  
Swine Model ◽  
Peripheral Blood Mononuclear ◽  
Cell Based Therapy

Background: Cardiac cell-based therapy (CCT) has emerged as a promising therapeutic strategy. There are few data comparing the fate of different stem cell (SC) populations delivered by the intracoronary injection (ICI). Aim: Document the in vivo myocardial distribution of SC delivered by ICI following recent myocardial infarction. Methods: In a myocardial infarction swine model, near-infrared (NIR) fluorescence was used to provide high sensitivity in vivo tracking of SC in the immediate phase (< 60 minutes) after ICI of 2x107 cells. After labeling with the NIR fluorophore, IR-786, the NIR signal intensities and myocardial distribution patterns of mesenchymal (MSC), bone marrow-(BMMNC) and peripheral blood-mononuclear (PBMNC) cells were compared using an open-chested fluorescence imaging system. Results: The SC populations of interest were successfully loaded with IR-786. While all SC populations readily distributed along the vascular territory of the infarct-related artery immediately after injection, there was a mean SI intensity drop of 29.8% and 14.1% for PBMNC and BMNC (p<0.05), respectively, which correlated with poor cell retention. No significant SI change was found in MSC-injected swine, which was associated with less cell loss after delivery. There was also evidence of MSC-related vessel plugging in some swine (Figure ). Conclusion: Our in vivo findings suggest that immediately after injection (during the first 60min ICI), there is already evidence of poor SC retention and distribution vary depending on cell population, potentially impacting clinical efficacy and safety of CCT.

scholarly journals Multimodality Animal Rotation Imaging System (MARS) for In Vivo Detection of Intraperitoneal Tumors

2011 ◽  
Vol 67 (1) ◽  
pp. 84-90 ◽  
Author(s):  
John Pizzonia ◽  
Jennie Holmberg ◽  
Sean Orton ◽  
Ayesha Alvero ◽  
Oscar Viteri ◽  
...  
Keyword(s):  
Imaging System ◽  
In Vivo Detection

Polarized hyperspectral imaging system for in vivo detection of vulvar lichen sclerosis

2016 ◽  
Author(s):  
Yingjie Qu ◽  
Wenqi Ren ◽  
Songde Liu ◽  
Peng Liu ◽  
Lan Xie ◽  
...  
Keyword(s):  
Hyperspectral Imaging ◽  
Imaging System ◽  
Lichen Sclerosis ◽  
In Vivo Detection
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