Transport Pathways of Solid Lipid Nanoparticles Across Madin–Darby Canine Kidney Epithelial Cell Monolayer

2014 ◽  
Vol 11 (10) ◽  
pp. 3716-3726 ◽  
Author(s):  
Gui-Hong Chai ◽  
Fu-Qiang Hu ◽  
Jihong Sun ◽  
Yong-Zhong Du ◽  
Jian You ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Solid Lipid Nanoparticles ◽  
Cell Monolayer ◽  
Lipid Nanoparticles ◽  
Madin Darby Canine Kidney ◽  
Transport Pathways ◽  
Solid Lipid ◽  
Kidney Epithelial Cell ◽  
Darby Canine Kidney ◽  
Canine Kidney

Transport features and structural optimization of solid lipid nanoparticles crossing the intestinal epithelium

RSC Advances ◽  
2016 ◽  
Vol 6 (74) ◽  
pp. 70433-70445 ◽  
Author(s):  
Guihong Chai ◽  
Yufang Meng ◽  
Shaoqing Chen ◽  
Fuqiang Hu ◽  
Yong Gan ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Structural Optimization ◽  
Intestinal Epithelium ◽  
Solid Lipid Nanoparticles ◽  
Intestinal Epithelial Cell ◽  
Cell Monolayer ◽  
Lipid Nanoparticles ◽  
Intestinal Epithelial ◽  
Solid Lipid

In vitro simulated intestinal epithelial cell monolayer is a novel avenue to screen optimal SLNs formulations.

scholarly journals Jaagsiekte Sheep Retrovirus Transformation in Madin-Darby Canine Kidney Epithelial Cell Three-Dimensional Culture

2010 ◽  
Vol 84 (10) ◽  
pp. 5379-5390 ◽  
Author(s):  
Chassidy Johnson ◽  
Kiah Sanders ◽  
Hung Fan
Keyword(s):  
Epithelial Cell ◽  
Mdck Cells ◽  
Mapk Pathway ◽  
Three Dimensional ◽  
Bronchioloalveolar Carcinoma ◽  
Envelope Gene ◽  
Madin Darby Canine Kidney ◽  
Jaagsiekte Sheep Retrovirus ◽  
Darby Canine Kidney ◽  
Canine Kidney

ABSTRACT Jaagsiekte sheep retrovirus (JSRV) is the causative agent of a contagious lung cancer in sheep that shares similarities with human bronchioloalveolar carcinoma (BAC). JSRV is unique because the envelope gene (env) is the oncogene, as it can transform cells in culture and induce tumors in animals. The phosphatidylinositol 3-kinase (PI3K)-Akt-mTOR and H/N-Ras-MEK-mitogen-activated protein kinase (MAPK) pathways have been shown to be critical for Env transformation. However, the question still remains of how disruption of these pathways relates to tumor formation. To address this, JSRV Env transformation was studied in the context of epithelial structure, using the polarized Madin-Darby canine kidney (MDCK) epithelial cell three-dimensional (3-D) culture system. The results indicated that JSRV Env-transformed MDCK cells were larger and had full or multiple lumens, in contrast to the single lumens observed in controls. The altered phenotype was largely mediated by an increase in proliferation, in addition to overcoming the proliferative suppression signal. JSRV Env was not found to disrupt polarity or tight junctions or to inhibit lumen apoptosis. The PI3K-Akt-mTOR pathway was important for Env transformation in MDCK cells, although the mechanisms of action differed in 3-D and monolayer cultures. PI3K-dependent signaling to mTOR occurred in monolayers, while PI3K-independent signaling to mTOR occurred in 3-D culture. In contrast, the H/N-Ras-MEK-MAPK pathway was found to be inhibitory to transformation in both normal and transformed MDCK cells in 3-D culture. However, in monolayer culture, inhibition of MEK reverted the transformed phenotype, suggesting a different mechanism(s) of action in monolayer versus 3-D culture.

scholarly journals Steady-state distribution and biogenesis of endogenous Madin-Darby canine kidney glycoproteins: evidence for intracellular sorting and polarized cell surface delivery.

1989 ◽  
Vol 109 (5) ◽  
pp. 2117-2127 ◽  
Author(s):  
M P Lisanti ◽  
A Le Bivic ◽  
M Sargiacomo ◽  
E Rodriguez-Boulan
Keyword(s):  
Steady State ◽  
Epithelial Cell ◽  
Cell Surface ◽  
Mdck Cells ◽  
Apical Surface ◽  
Steady State Distribution ◽  
State Distribution ◽  
Madin Darby Canine Kidney ◽  
Darby Canine Kidney ◽  
Canine Kidney

We used domain-selective biotinylation/125I-streptavidin blotting (Sargiacomo, M., M. P. Lisanti, L. Graeve, A. Le Bivic, and E. Rodriguez-Boulan. 1989 J. Membr. Biol. 107:277-286), in combination with lectin precipitation, to analyze the apical and basolateral glycoprotein composition of Madin-Darby canine kidney (MDCK) cells and to explore the role of glycosylation in the targeting of membrane glycoproteins. All six lectins used recognized both apical and basolateral glycoproteins, indicating that none of the sugar moieties detected were characteristic of the particular epithelial cell surface. Pulse-chase experiments coupled with domain-selective glycoprotein recovery were designed to detect the initial appearance of newly synthesized glycoproteins at the apical or basolateral cell surface. After a short pulse with a radioactive precursor, glycoproteins reaching each surface were biotinylated, extracted, and recovered via precipitation with immobilized streptavidin. Several basolateral glycoproteins (including two sulfated proteins) and at least two apical glycoproteins (one of them the major sulfated protein of MDCK cells) appeared at the corresponding surface after 20-40 min of chase, but were not detected in the opposite surface, suggesting that they were sorted intracellularly and vectorially delivered to their target membrane. Several "peripheral" apical proteins were detected at maximal levels on the apical surface immediately after the 15-min pulse, suggesting a very fast intracellular transit. Finally, domain-selective labeling of surface carbohydrates with biotin hydrazide (after periodate oxidation) revealed strikingly different integral and peripheral glycoprotein patterns, resembling the Con A pattern, after labeling with sulfo-N-hydroxy-succinimido-biotin. The approaches described here should be useful in characterizing the steady-state distribution and biogenesis of endogenous cell surface components in a variety of epithelial cell lines.

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