Epidermal Stem Cells Manipulated by pDNA-VEGF165/CYD-PEI Nanoparticles Loaded Gelatin/β-TCP Matrix as a Therapeutic Agent and Gene Delivery Vehicle for Wound Healing

2013 ◽  
Vol 10 (8) ◽  
pp. 3090-3102 ◽  
Author(s):  
Li-Hua Peng ◽  
Wei Wei ◽  
Xiao-Tian Qi ◽  
Ying-Hui Shan ◽  
Fang-Jun Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Gene Delivery ◽  
Therapeutic Agent ◽  
Epidermal Stem Cells ◽  
Delivery Vehicle ◽  
Gene Delivery Vehicle ◽  
Pei Nanoparticles

scholarly journals Reducing alcohol and/or cocaine-induced reward and toxicity via an epidermal stem cell-based gene delivery platform

2021 ◽  
Author(s):  
Qingyao Kong ◽  
Yuanyuan Li ◽  
Jiping Yue ◽  
Xiaoyang Wu ◽  
Ming Xu
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Gene Delivery ◽  
Unmet Need ◽  
Skin Grafts ◽  
Epidermal Stem Cell ◽  
Epidermal Stem Cells ◽  
Concurrent Use ◽  
Delivery Platform ◽  
Glucagon Like Peptide 1

AbstractAlcohol use disorder (AUD) is one of the foremost public health problems. Alcohol is also frequently co-abused with cocaine. There is a huge unmet need for the treatment of AUD and/or cocaine co-abuse. We recently demonstrated that skin grafts generated from mouse epidermal stem cells that had been engineered by CRISPR-mediated genome editing could be transplanted onto mice as a gene delivery platform. Here, we show that expression of the glucagon-like peptide-1 (GLP1) gene delivered by epidermal stem cells attenuated development and reinstatement of alcohol-induced drug-taking and seeking as well as voluntary oral alcohol consumption. GLP1 derived from the skin grafts decreased alcohol-induced increase in dopamine levels in the nucleus accumbens. In exploring the potential of this platform in reducing concurrent use of drugs, we developed a novel co-grafting procedure for both modified human butyrylcholinesterase (hBChE)- and GLP1-expressing cells. Epidermal stem cell-derived hBChE and GLP1 reduced acquisition of drug-taking and toxicity induced by alcohol and cocaine co-administration. These results imply that cutaneous gene delivery through skin transplants may add a new option to treat drug abuse and co-abuse.

scholarly journals Epidermal Stem Cells in Skin Wound Healing

2017 ◽  
Vol 6 (9) ◽  
pp. 297-307 ◽  
Author(s):  
Yuanyuan Li ◽  
Jamie Zhang ◽  
Jiping Yue ◽  
Xuewen Gou ◽  
Xiaoyang Wu
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Skin Wound ◽  
Epidermal Stem Cells ◽  
Skin Wound Healing
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