scholarly journals Asymmetrical N4,N9-Diacyl Spermines: SAR Studies of Nonviral Lipopolyamine Vectors for Efficient siRNA Delivery with Silencing of EGFP Reporter Gene

2012 ◽  
Vol 9 (7) ◽  
pp. 1853-1861 ◽  
Author(s):  
Ian S. Blagbrough ◽  
Abdelkader A. Metwally ◽  
Hassan M. Ghonaim
Keyword(s):  
Reporter Gene ◽  
Sirna Delivery ◽  
Sar Studies ◽  
Egfp Reporter Gene ◽  
Egfp Reporter

Combined sense-antisense Alu elements activate the EGFP reporter gene when stable transfection

2017 ◽  
Vol 292 (4) ◽  
pp. 833-846 ◽  
Author(s):  
Zhihong Ma ◽  
Xianglong Kong ◽  
Shufeng Liu ◽  
Shuxian Yin ◽  
Yuehua Zhao ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Stable Transfection ◽  
Alu Elements ◽  
Egfp Reporter Gene ◽  
Egfp Reporter

Development of the 5-HT2CR-Tango System Combined with an EGFP Reporter Gene

2015 ◽  
Vol 58 (2) ◽  
pp. 162-169 ◽  
Author(s):  
Yoshihisa Watanabe ◽  
Atsushi Tsujimura ◽  
Miku Aoki ◽  
Katsutoshi Taguchi ◽  
Masaki Tanaka
Keyword(s):  
Reporter Gene ◽  
Egfp Reporter Gene ◽  
Egfp Reporter

oct4 -EGFP reporter gene expression marks the stem cells in embryonic development and in adult gonads of transgenic medaka

2012 ◽  
Vol 80 (1) ◽  
pp. 48-58 ◽  
Author(s):  
Alexander Froschauer ◽  
Mst. Muslima Khatun ◽  
David Sprott ◽  
Alexander Franz ◽  
Christiane Rieger ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Development ◽  
Reporter Gene ◽  
Reporter Gene Expression ◽  
Egfp Reporter Gene ◽  
Egfp Reporter

scholarly journals Quantitative Silencing of EGFP Reporter Gene by Self-Assembled siRNA Lipoplexes of LinOS and Cholesterol

2012 ◽  
Vol 9 (11) ◽  
pp. 3384-3395 ◽  
Author(s):  
Abdelkader A. Metwally ◽  
Ian S. Blagbrough ◽  
Judith M. Mantell
Keyword(s):  
Reporter Gene ◽  
Egfp Reporter Gene ◽  
Egfp Reporter ◽  
Self Assembled

scholarly journals Male and female germline specific expression of an EGFP reporter gene in a unique strain of transgenic rats

2005 ◽  
Vol 284 (1) ◽  
pp. 171-183 ◽  
Author(s):  
Jennifer T. Cronkhite ◽  
Carola Norlander ◽  
Jenny K. Furth ◽  
Göran Levan ◽  
David L. Garbers ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Transgenic Rats ◽  
Specific Expression ◽  
Male And Female ◽  
Egfp Reporter Gene ◽  
Egfp Reporter ◽  
Unique Strain ◽  
Female Germline

scholarly journals Efficient Silencing of EGFP Reporter Gene with siRNA Delivered by Asymmetrical N4,N9-Diacyl Spermines

2012 ◽  
Vol 9 (7) ◽  
pp. 1862-1876 ◽  
Author(s):  
Abdelkader A. Metwally ◽  
Olivier Reelfs ◽  
Charareh Pourzand ◽  
Ian S. Blagbrough
Keyword(s):  
Reporter Gene ◽  
Egfp Reporter Gene ◽  
Egfp Reporter

scholarly journals Loss of Posterior Silk Gland Transcription Specificity of Fibroin Light Chain Promoter due to Absence of 41 bp Sequence Containing Possible Inhibitor Binding Sites

2005 ◽  
Vol 37 (12) ◽  
pp. 819-825 ◽  
Author(s):  
Ting-Qing Guo ◽  
Jian-Yang Wang ◽  
Sheng-Peng Wang ◽  
Xiu-Yang Guo ◽  
Ke-Wei Huang ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Light Chain ◽  
Binding Sites ◽  
Fluorescent Protein ◽  
Silk Gland ◽  
Inhibitor Binding ◽  
Egfp Reporter Gene ◽  
Egfp Reporter ◽  
Fibroin Light Chain ◽  
Posterior Silk Gland

Abstract The gene encoding fibroin light chain protein (FibL) is specifically expressed in the posterior silk gland of silkworm and repressed in other tissues. The binding sites of several transcription factors involved in the silk gland transcription specificity of fibl promoter have been recognized, including SGFB, PSGF and BMFA. Here we report the leak expression of the enhanced green fluorescent protein (EGFP) reporter gene in tissues other than the posterior silk gland in vivo when under the control of a shortened fibl promoter with deletion of the 5′ terminal 41 bp sequence, which is located at −650 nt to −610 nt upstream of the fibl transcription starting site. Assay of silk gland specificity of the promoters was performed by observation of green fluorescence in tissues of silkworm larvae following inter-haemocoelic injection of recombinant Autographa californica multiple nuclear polyhedrosis virus carrying the EGFP reporter gene controlled by different lengths of fibl promoters. Our results indicated that availability of the binding sites of several known factors, including SGFB, PSGF and BMFA, is not sufficient for intact silk gland transcription specificity of fibl promoter, and there are possible inhibitor binding sites in the 41 bp sequence (−650 nt to −610 nt) upstream of the transcription starting site which may be required to repress the activity of fibl promoter in other tissues.

scholarly journals Identification of Novel Inhibitors of the Type I Interferon Induction Pathway Using Cell-Based High-Throughput Screening

2016 ◽  
Vol 21 (9) ◽  
pp. 978-988 ◽  
Author(s):  
Zoe O. Gage ◽  
Andri Vasou ◽  
David W. Gray ◽  
Richard E. Randall ◽  
Catherine S. Adamson
Keyword(s):  
Small Molecules ◽  
Reporter Gene ◽  
High Throughput ◽  
High Throughput Screening ◽  
Fluorescent Protein ◽  
Type I Interferon ◽  
Type I ◽  
Egfp Reporter Gene ◽  
Egfp Reporter ◽  
Green Fluorescent

Production of type I interferon (IFN) is an essential component of the innate immune response against invading pathogens. However, its production must be tightly regulated to avoid harmful effects. Compounds that modulate the IFN response are potentially valuable for a variety of applications due to IFN’s beneficial and detrimental roles. We developed and executed a cell-based high-throughput screen (HTS) targeting components that participate in and/or regulate the IRF3 and nuclear factor (NF)–κB branches of the IFN induction pathway. The assay detects activation of the IFN induction pathway via an enhanced green fluorescent protein (eGFP) reporter gene under the control of the IFNβ promoter and was optimized, miniaturized, and demonstrated suitable for HTS as robust Z′ factor scores of >0.6 were consistently achieved. A diversity screening set of 15,667 small molecules was assayed and two novel hit compounds validated that specifically inhibit the IFN induction pathway. We demonstrate that one of these compounds acts at or upstream of IRF3 phosphorylation. A second cell-based assay to detect activation of the IFN signaling (Jak-Stat) pathway via an eGFP reporter gene under the control of an IFN-stimulated response element (ISRE) containing MxA promoter also performed well (robust Z′ factor >0.7) and may therefore be similarly used to identify small molecules that modulate the IFN signaling pathway.

Nuclear Transfer of Blastomeres Expressing EGFP-Reporter Gene May Improve the Efficiency of Transgenic Cattle

2001 ◽  
Vol 3 (4) ◽  
pp. 183-190 ◽  
Author(s):  
H. Funahashi ◽  
A. Ideta ◽  
M. Konishi ◽  
M. Urakawa ◽  
K. Uruno ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Nuclear Transfer ◽  
Transgenic Cattle ◽  
Egfp Reporter Gene ◽  
Egfp Reporter
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