scholarly journals Genetically Modified Mesenchymal Stem Cells for Improved Islet Transplantation

2011 ◽  
Vol 8 (5) ◽  
pp. 1458-1470 ◽  
Author(s):  
Hao Wu ◽  
Zhaoyang Ye ◽  
Ram I. Mahato
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Islet Transplantation ◽  
Genetically Modified

scholarly journals Therapeutic potential of genetically modified mesenchymal stem cells

Gene Therapy ◽  
2008 ◽  
Vol 15 (10) ◽  
pp. 711-715 ◽  
Author(s):  
S Kumar ◽  
D Chanda ◽  
S Ponnazhagan
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Therapeutic Potential ◽  
Genetically Modified

Electric-Pulse Current Stimulation Increases If Current in mShox2 Genetically Modified Canine Mesenchymal Stem Cells

Cardiology ◽  
2015 ◽  
Vol 132 (1) ◽  
pp. 49-57 ◽  
Author(s):  
Yuanyuan Feng ◽  
Shouming Luo ◽  
Shifei Tong ◽  
Li Zhong ◽  
Changhai Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Fluorescent Protein ◽  
Pulse Current ◽  
Morphological Changes ◽  
Electric Pulse ◽  
Genetically Modified ◽  
Inward Current ◽  
Cardiomyogenic Differentiation ◽  
If Current

Objective: We aimed to investigate the role of mShox2 in generating If pacemaker current in vitro by means of electric-pulse current stimulation (EPCS) of canine mesenchymal stem cells (cMSCs). Methods: mShox2 genetically modified cMSCs were prepared with pLentis-mShox2 red fluorescent protein. After EPCS induction, we examined the kinetic characteristics of generated inward current by means of a patch clamp. We then evaluated the expression of pacemaker-related genes, such as Nkx2.5, Tbx3, HCN4, Cx43 and Cx45, by means of qRT-PCR and Western blotting. The morphological changes and the cardiomyogenic differentiation marker cTnT were investigated at the same time. Results: The time- and voltage-dependent inward current recorded after mShox2 infection was confirmed to be If current. After EPCS induction, the detection rate of this If current was increased. The current amplitude and density were increased, and the channel activation curve shifted to the right. The pacemaker markers Tbx3, HCN4 and Cx45 were significantly upregulated, but the working myocardium markers Nkx2.5 and Cx43 were downregulated after mShox2 infection, and were more remarkable after EPCS induction. The cells became larger and assumed spindle and spider-like morphologies. cTnT was also detected in the experimental cells. Conclusions: Our results suggest that EPCS promotes the differentiation of mShox2 genetically modified cMSCs into pacemaker-like cells, which generates more If current.

scholarly journals IFNα-Expressing Amniotic Fluid-Derived Mesenchymal Stem Cells Migrate to and Suppress HeLa Cell-Derived Tumors in a Mouse Model

2018 ◽  
Vol 2018 ◽  
pp. 1-14 ◽  
Author(s):  
Jun Zhou ◽  
Tian Liang ◽  
Dejun Wang ◽  
Liru Li ◽  
Yan Cheng ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mouse Model ◽  
Tumor Growth ◽  
Tumor Cell ◽  
Hela Cell ◽  
Amniotic Fluid ◽  
Genetically Modified ◽  
Tumor Tropism

Background. Immunotherapy for cervical cancer with type I interferon (IFN) is limited because of the cytotoxicity that accompanies the high doses that are administered. In this study, we investigated the utilization of amniotic fluid-derived mesenchymal stem cells (AF-MSCs) as a means for delivering IFNα to local tumor sites for the suppression of cervical cancer in a mouse model using HeLa cell xenografts. Methods. The tumor tropism ability of AF-MSCs and AF-MSCs genetically modified to overexpress IFNα (IFNα-AF-MSCs) was examined through Transwell in vitro and through fluorescent images and immunohistochemistry in a mouse model. Tumor size and tumor apoptosis were observed to evaluate the efficacy of the targeting therapy. Mechanistically, tumor cell apoptosis was detected by cytometry and TUNEL, and oncogenic proteins c-Myc, p53, and Bcl-2 as well as microvessel density were detected by immunohistochemistry. Results. In this model, intravenously injected AF-MSCs selectively migrated to the tumor sites, participated in tumor construction, and promoted tumor growth. After being genetically modified to overexpress IFNα, the IFNα-AF-MSCs maintained their tumor tropism but could significantly suppress tumor growth. The restrictive efficacy of IFNα-AF-MSCs was associated with the suppression of angiogenesis, inhibition of tumor cell proliferation, and induction of apoptosis in tumor cells. Neither AF-MSCs nor IFNα-AF-MSCs trigger tumor formation. Conclusions. IFNα-AF-MSC-based therapy is feasible and shows potential for treating cervical cancer, suggesting that AF-MSCs may be promising vehicles for delivering targeted anticancer therapy.

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