Molecular Design and in Vitro Studies of Novel pH-Sensitive Hydrogels for the Oral Delivery of Calcitonin

1999 ◽  
Vol 32 (20) ◽  
pp. 6646-6651 ◽  
Author(s):  
Madeline Torres-Lugo ◽  
Nikolaos A. Peppas
Keyword(s):  
Oral Delivery ◽  
Molecular Design ◽  
Ph Sensitive ◽  
In Vitro Studies

pH-sensitive thiolated nanoparticles facilitate the oral delivery of insulin in vitro and in vivo

2015 ◽  
Vol 17 (2) ◽  
Author(s):  
Yan Zhang ◽  
Xia Lin ◽  
Xuli Du ◽  
Sicong Geng ◽  
Hongren Li ◽  
...  
Keyword(s):  
Oral Delivery ◽  
Ph Sensitive

scholarly journals Garcinol Encapsulated Ph-Sensitive Biodegradable Nanoparticles: A Novel Therapeutic Strategy for the Treatment of Inflammatory Bowel Disease

Polymers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 862
Author(s):  
Eden Mariam Jacob ◽  
Ankita Borah ◽  
Sindhu C. Pillai ◽  
D. Sakthi Kumar
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Oral Delivery ◽  
Substantial Improvement ◽  
In Vitro Cytotoxicity ◽  
Ph Sensitive ◽  
Tnf Α ◽  
Evaporation Technique ◽  
Inflammatory Bowel

The emergence of pH-sensitive nanoscale particles is beneficial due to their ability to only release cargo in a colonic pH environment, which helps to directly target inflamed tissues in inflammatory bowel disease (IBD). Hence, we have designed the formulation of pH-sensitive biodegradable garcinol (GAR)-loaded poly (lactic–co–glycolic acid) (PLGA) coated with Eudragit® S100 (ES100) (GAR-PLGA-ES100 nanoparticles (NPs)) for reducing inflammation caused by proinflammatory cytokines. The GAR-PLGA-ES100 NPs were prepared using a solvent evaporation technique and characterized for shape and surface morphology. An in vitro drug release study revealed the release of the drug specifically from NPs at the colonic pH of 7.4. The in vitro cytotoxicity of the GAR-PLGA-ES100 NPs was also evaluated and found to be highly biocompatible with CACO-2 cells. These NPs were able to reduce lactate dehydrogenase (LDH) and myeloperoxidase (MPO) activity. Inhibition of the expression of pro-inflammatory cytokine TNF-α , chemokine interleukin (IL)-8 and the nuclear factor kappa light chain enhancer of activated B-cells (NF-κB) was observed after GAR-PLGA-ES100 NPs treatment. Therefore, our results support the idea that GAR-PLGA-ES100 NPs show substantial improvement after the release of the drug, specifically in colonic pH targeting and reduction in the activation of inflammation that leads to IBD, suggesting that GAR-PLGA-ES100 NPs are promising candidates for oral delivery to colonic inflamed tissue.

Insulin Loaded Eudragit L100 Microspheres for Oral Delivery: Preliminary in vitro Studies

2006 ◽  
Vol 21 (2) ◽  
pp. 195-211 ◽  
Author(s):  
Deepti Jain ◽  
D. K. Majumdar ◽  
A. K. Panda
Keyword(s):  
Oral Delivery ◽  
In Vitro Studies ◽  
Eudragit L100

Preparation and Characterization of Insulin Nanoparticles Employing Chitosan and Poly(methylmethacrylate/methylmethacrylic acid) Copolymer

2006 ◽  
Vol 6 (9) ◽  
pp. 2874-2886 ◽  
Author(s):  
Ming-Guang Li ◽  
Wan-Liang Lu ◽  
Jian-Cheng Wang ◽  
Xuan Zhang ◽  
Hua Zhang ◽  
...  
Keyword(s):  
Oral Delivery ◽  
Electrostatic Interactions ◽  
Release Kinetics ◽  
Ph Sensitive ◽  
Release Process ◽  
Eudragit L100 ◽  
Acid Copolymer ◽  
Nanoparticle System ◽  
Modeling Data

As most of polypeptides are marginally stable, a mild formulation procedure would be beneficial for the activities of these drugs. The objective of the present study was to develop a novel pH-sensitive nanoparticle system that was suitable for entrapment of hydrophilic insulin but without affecting its conformation. Chitosan was incorporated as a positively charged material, and one of the three poly(methylmethacrylate/methylmethacrylic acid) copolymers, consisting of Eudragit L100-55, L100, and S100, was used as a negatively charged polymer for preparation of three insulin nanoparticles, respectively. Three nanoparticles obtained were spherical. The mean diameters were in the range from 200 nm to 250 nm, and the entrapment efficiencies, from 50% to 70%. The surface analysis indicated that insulin was evenly distributed in the nanoparticles. Polymer ratio of chitosan to Eudragit was the factor which influenced the nanoparticles significantly. Characterization results showed that the electrostatic interactions existed, thus providing a mild formulation procedure which did not affect the chemical integrity and the conformation of insulin. In vitro release studies revealed that all three types of the nanoparticles exhibited a pH-dependant characteristic. The modeling data indicated that the release kinetics of insulin was nonlinear, and during the release process, the nanoparticles showed a polynomial swelling. On overall estimation, the insulin chitosan-Eudragit L100-55 nanoparticles may be better for the oral delivery. This new pH-sensitive nanoparticle formulation using chitosan and Eudragit L100-55 polymer may provide a useful approach for entrapment of hydrophilic polypeptides without affecting their conformation.

In vitro - studies with antler bone cells: Structure forming capacity, osteocalcin production and influence of sex steroids

2006 ◽  
Vol 15 (04) ◽  
pp. 245-257 ◽  
Author(s):  
H. J. Rolf ◽  
K. G. Wiese ◽  
H. Siggelkow ◽  
H. Schliephake ◽  
G. A. Bubernik
Keyword(s):  
Sex Steroids ◽  
Bone Cells ◽  
In Vitro Studies ◽  
Osteocalcin Production
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