Two-Dimensional Polymerization of Lipid Bilayers:  Effect of Lipid Lateral Diffusion on the Rate and Degree of Polymerization

1999 ◽  
Vol 32 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Junting Lei ◽  
Thomas M. Sisson ◽  
Henry G. Lamparski ◽  
David F. O'Brien
Keyword(s):  
Lipid Bilayers ◽  
Lateral Diffusion ◽  
Degree Of Polymerization ◽  
Two Dimensional

Molecular and global structure and dynamics of membranes and lipid bilayers

1990 ◽  
Vol 68 (9) ◽  
pp. 999-1012 ◽  
Author(s):  
E. Sackmann
Keyword(s):  
Lipid Bilayers ◽  
Plasma Membranes ◽  
Mechanical Stability ◽  
Dynamic Properties ◽  
Composite Membranes ◽  
Two Dimensional ◽  
Molecular Architecture ◽  
Present Contribution ◽  
Cell Plasma ◽  
Low Dimensionality

The cell plasma is a composite type of material that is made up of a two-dimensional liquid crystal (lipid–protein bilayer) to which a macromolecular network (the cytoskeleton) is loosely coupled. The latter may be approximately two dimensional as in the case of the erythrocytes or may extend throughout the whole cell cytoplasm. Owing to this combination of two states of matter, the membrane combines the dynamics and flexibility of a fluid with the mechanical stability of a solid. Owing to its low dimensionality, the local structure of the bilayer or the global shape of cells may be most effectively controlled and modulated by biochemical signals such as macromolecular adsorption. The present contribution deals with comparative studies of the local and global dynamic properties of biological and artificial membranes. In the first part the question of the physical basis of selective lipid–protein interaction mechanisms is addressed and the outstanding viscoelastic properties of plasma membranes and their role for local instabilities shape fluctuations of cells and the cell–substrate interaction are described. The second part deals with the molecular architecture and dynamics of composite membranes prepared by combining monomeric and macromolecular lipids. These model membranes open new possibilities to mimick complex mechanical processes of cell plasma membranes and to prepare low-dimensionality macromolecular solutions and gels. Finally, the use of such compound systems by nature to prepare the semipermeable protective layers of plant leaves, the so-called cuticle, is discussed. In analogy to plasma membranes, the local transport properties are modulated by variation of the liquid-crystalline state of the monomeric waxes.

scholarly journals Peptide-presenting two-dimensional protein matrix on supported lipid bilayers: An efficient platform for cell adhesion

2007 ◽  
Vol 2 (4) ◽  
pp. 165-172 ◽  
Author(s):  
Rémi Bérat ◽  
Murielle Rémy-Zolghadry ◽  
Céline Gounou ◽  
Claude Manigand ◽  
Sisareuth Tan ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Lipid Bilayers ◽  
Supported Lipid Bilayers ◽  
Two Dimensional ◽  
Protein Matrix

scholarly journals Visualization of CD2 interaction with LFA-3 and determination of the two-dimensional dissociation constant for adhesion receptors in a contact area.

1996 ◽  
Vol 132 (3) ◽  
pp. 465-474 ◽  
Author(s):  
M L Dustin ◽  
L M Ferguson ◽  
P Y Chan ◽  
T A Springer ◽  
D E Golan
Keyword(s):  
Contact Area ◽  
Dissociation Constants ◽  
Three Dimensional ◽  
Lateral Diffusion ◽  
Solution Phase ◽  
Phospholipid Bilayer ◽  
Cell Contact ◽  
Two Dimensional ◽  
Adhesion Receptors ◽  
Dimensional Solution

Many adhesion receptors have high three-dimensional dissociation constants (Kd) for counter-receptors compared to the KdS of receptors for soluble extracellular ligands such as cytokines and hormones. Interaction of the T lymphocyte adhesion receptor CD2 with its counter-receptor, LFA-3, has a high solution-phase Kd (16 microM at 37 degrees C), yet the CD2/LFA-3 interaction serves as an effective adhesion mechanism. We have studied the interaction of CD2 with LFA-3 in the contact area between Jurkat T lymphoblasts and planar phospholipid bilayers containing purified, fluorescently labeled LFA-3. Redistribution and lateral mobility of LFA-3 were measured in contact areas as functions of the initial LFA-3 surface density and of time after contact of the cells with the bilayers. LFA-3 accumulated at sites of contact with a half-time of approximately 15 min, consistent with the previously determined kinetics of adhesion strengthening. The two-dimensional Kd for the CD2/LFA-3 interaction was 21 molecules/microns 2, which is lower than the surface densities of CD2 on T cells and LFA-3 on most target or stimulator cells. Thus, formation of CD2/LFA-3 complexes should be highly favored in physiological interactions. Comparison of the two-dimensional (membrane-bound) and three-dimensional (solution-phase) KdS suggest that cell-cell contact favors CD2/LFA-3 interaction to a greater extent than that predicted by the three-dimensional Kd and the intermembrane distance at the site of contact. LFA-3 molecules in the contact site were capable of lateral diffusion in the plane of the phospholipid bilayer and did not appear to be irreversibly trapped in the contact area, consistent with a rapid off-rate. These data provide insights into the function of low affinity interactions in adhesion.

scholarly journals On the Kinetics of Adsorption and Two-Dimensional Self-Assembly of Annexin A5 on Supported Lipid Bilayers

2005 ◽  
Vol 89 (5) ◽  
pp. 3372-3385 ◽  
Author(s):  
Ralf P. Richter ◽  
Joséphine Lai Kee Him ◽  
Béatrice Tessier ◽  
Céline Tessier ◽  
Alain R. Brisson
Keyword(s):  
Lipid Bilayers ◽  
Self Assembly ◽  
Supported Lipid Bilayers ◽  
Annexin A5 ◽  
Two Dimensional ◽  
Kinetics Of Adsorption ◽  
Kinetics Of
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