Protein Resistance of Surfaces Prepared by Chemisorption of Monothiolated Poly(ethylene glycol) to Gold and Dendronization with Aliphatic Polyester Dendrons: Effect of Hydrophilic Dendrons

2008 ◽  
Vol 41 (7) ◽  
pp. 2567-2576 ◽  
Author(s):  
S. Rahima Benhabbour ◽  
Lina Liu ◽  
Heather Sheardown ◽  
Alex Adronov
Keyword(s):  
Ethylene Glycol ◽  
Aliphatic Polyester ◽  
Poly Ethylene Glycol ◽  
Protein Resistance ◽  
Poly Ethylene

scholarly journals Protein resistance of dextran and dextran-poly(ethylene glycol) copolymer films

Biofouling ◽  
2011 ◽  
Vol 27 (5) ◽  
pp. 497-503 ◽  
Author(s):  
Darby Kozak ◽  
Annie Chen ◽  
Jacinda Bax ◽  
Matt Trau
Keyword(s):  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Protein Resistance ◽  
Copolymer Films ◽  
Poly Ethylene

Biodegradable Polyester of Poly (Ethylene glycol)-sebacic Acid as a Backbone for β -Cyclodextrin-polyrotaxane: A Promising Gene Silencing Vector

2019 ◽  
Vol 19 (4) ◽  
pp. 274-287
Author(s):  
Sharwari Ghodke ◽  
Prajakta Mahajan ◽  
Kritika Gupta ◽  
Chilukuri Ver Avadhani ◽  
Prajakta Dandekar ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Ethylene Glycol ◽  
Gene Silencing ◽  
Biomedical Applications ◽  
Sebacic Acid ◽  
Water Soluble ◽  
Cellular Cytotoxicity ◽  
Aliphatic Polyester ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Background: Polyrotaxane, a macromolecular interlocked assembly, consisting of cyclodextrin has excellent inclusion capabilities and functionalization capacity, which makes it a versatile material as a vector for gene delivery applications. Objective: A biodegradable linear aliphatic polyester axle composed of Polyethylene Glycol (PEG) and Sebacic Acid (SA) was used to fabricate the β-Cyclodextrin (β-CD) based polyrotaxane as a cationic polymeric vector and evaluated for its potential gene silencing efficiency. Methods: The water-soluble aliphatic polyester was synthesized by the solvent esterification process and characterized using viscometry, GPC, FT-IR and 1H NMR spectroscopy. The synthesized polyester was further evaluated for its biodegradability and cellular cytotoxicity. Hence, this water-soluble polyester was used for the step-wise synthesis of polyrotaxane, via threading and blocking reactions. Threading of β-CD over PEG-SA polyester axle was conducted in water, followed by end-capping of polypseudorotaxane using 2,4,6-trinitrobenzenesulfonic acid to yield polyester-based polyrotaxane. For gene delivery application, cationic polyrotaxane (PRTx+) was synthesized and evaluated for its gene loading and gene silencing efficiency. Results and Discussion: The resulting novel macromolecular assembly was found to be safe for use in biomedical applications. Further, characterization by GPC and 1H NMR techniques revealed successful formation of PE-β-CD-PRTx with a threading efficiency of 16%. Additionally, the cellular cytotoxicity assay indicated biosafety of the synthesized polyrotaxane, exploring its potential for gene delivery and other biomedical applications. Further, the biological profile of PRTx+: siRNA complexes was evaluated by measuring their zeta potential and gene silencing efficiency, which were found to be comparable to Lipofectamine 3000, the commercial transfecting agent. Conclusion: The combinatory effect of various factors such as biodegradability, favourable complexation ability, near zero zeta potentials, good cytotoxicity properties of poly (ethylene glycol)-sebacic acid based β-Cyclodextrin-polyrotaxane makes it a promising gene delivery vector for therapeutic applications.

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