Single Molecule Force Spectroscopy on Polyelectrolytes:  Effect of Spacer on Adhesion Force and Linear Charge Density on Rigidity

2004 ◽  
Vol 37 (3) ◽  
pp. 946-953 ◽  
Author(s):  
Shuxun Cui ◽  
Chuanjun Liu ◽  
Zhiqiang Wang ◽  
Xi Zhang ◽  
Satu Strandman ◽  
...  
Keyword(s):  
Charge Density ◽  
Single Molecule ◽  
Adhesion Force ◽  
Force Spectroscopy ◽  
Linear Charge ◽  
Single Molecule Force Spectroscopy ◽  
Linear Charge Density

scholarly journals Ultrahigh Adhesion Force Between Silica-Binding Peptide SB7 and Glass Substrate Studied by Single-Molecule Force Spectroscopy and Molecular Dynamic Simulation

2020 ◽  
Vol 8 ◽  
Author(s):  
Xiaoxu Zhang ◽  
Jialin Chen ◽  
Enci Li ◽  
Chunguang Hu ◽  
Shi-Zhong Luo ◽  
...  
Keyword(s):  
Single Molecule ◽  
Glass Surface ◽  
Silica Surface ◽  
Adhesion Force ◽  
Force Spectroscopy ◽  
Dynamics Simulation ◽  
Single Molecule Force Spectroscopy ◽  
Binding Peptide ◽  
Single Molecule Level ◽  
Arginine Residues

Many proteins and peptides have been identified to effectively and specifically bind on certain surfaces such as silica, polystyrene and titanium dioxide. It is of great interest, in many areas such as enzyme immobilization, surface functionalization and nanotechnology, to understand how these proteins/peptides bind to solid surfaces. Here we use single-molecule force spectroscopy (SMFS) based on atomic force microscopy to directly measure the adhesion force between a silica-binding peptide SB7 and glass surface at single molecule level. SMFS results show that the adhesion force of a single SB7 detaching from the glass surface distributes in two populations at ~220 pN and 610 pN, which is higher than the unfolding forces of most mechanically stable proteins and the unbinding forces of most stable protein-protein interactions. Molecular dynamics simulation reveals that the electrostatic interactions between positively charged arginine residues and the silica surface dominates the binding of SB7 on silica. Our study provides experimental evidence and molecular mechanism at the single-molecule level for the SB7-based immobilization of proteins on silica-based surface, which is able to withstand high mechanical forces, making it an ideal fusion tag for silica surface immobilization or peptide-base adhesive materials.

scholarly journals Single Molecule Force Spectroscopy of Siloxanes

2021 ◽  
Vol 714 (3) ◽  
pp. 032023
Author(s):  
Ling Chen ◽  
Liya Yang ◽  
Chunxia Wang ◽  
Ting Zhu
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy

Pulling Genetic RNA out of Tobacco Mosaic Virus Using Single-Molecule Force Spectroscopy

2010 ◽  
Vol 132 (32) ◽  
pp. 11036-11038 ◽  
Author(s):  
Ningning Liu ◽  
Bo Peng ◽  
Yuan Lin ◽  
Zhaohui Su ◽  
Zhongwei Niu ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy

The Nature of the Force-Induced Conformation Transition of dsDNA Studied by Using Single Molecule Force Spectroscopy

Langmuir ◽  
2010 ◽  
Vol 26 (12) ◽  
pp. 9491-9496 ◽  
Author(s):  
Ningning Liu ◽  
Tianjia Bu ◽  
Yu Song ◽  
Wei Zhang ◽  
Jinjing Li ◽  
...  
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy ◽  
Conformation Transition

Peptide Desorption Kinetics from Single Molecule Force Spectroscopy Studies

2014 ◽  
Vol 136 (2) ◽  
pp. 688-697 ◽  
Author(s):  
Stefanie Krysiak ◽  
Susanne Liese ◽  
Roland R. Netz ◽  
Thorsten Hugel
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Desorption Kinetics ◽  
Single Molecule Force Spectroscopy ◽  
Spectroscopy Studies

scholarly journals Quantifying DNA melting transitions using single-molecule force spectroscopy

2008 ◽  
Vol 21 (3) ◽  
pp. 034114 ◽  
Author(s):  
Christopher P Calderon ◽  
Wei-Hung Chen ◽  
Kuan-Jiuh Lin ◽  
Nolan C Harris ◽  
Ching-Hwa Kiang
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Dna Melting ◽  
Single Molecule Force Spectroscopy ◽  
Melting Transitions
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