Light scattering study of three-component systems. 1. Excluded volume effect of poly(methyl methacrylate) in the binary mixture 1-chlorobutane + 2-methoxyethanol

1985 ◽  
Vol 18 (9) ◽  
pp. 1736-1740 ◽  
Author(s):  
Mitsuo Nakata ◽  
Naoko Numazawa
Keyword(s):  
Light Scattering ◽  
Binary Mixture ◽  
Methyl Methacrylate ◽  
Volume Effect ◽  
Excluded Volume ◽  
Poly Methyl Methacrylate ◽  
Component Systems ◽  
Excluded Volume Effect ◽  
Scattering Study

MACROMOLECULAR CROWDING IN BIOLOGICAL SYSTEMS: DYNAMIC LIGHT SCATTERING (DLS) TO QUANTIFY THE EXCLUDED VOLUME EFFECT (EVE)

2006 ◽  
Vol 01 (03) ◽  
pp. 317-325 ◽  
Author(s):  
KARTHIK S. HARVE ◽  
MICHAEL RAGHUNATH ◽  
RICKY R. LAREU ◽  
RAJ RAJAGOPALAN
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Biological Systems ◽  
Volume Effect ◽  
Threshold Concentration ◽  
Excluded Volume ◽  
Matrix Deposition ◽  
Excluded Volume Effect

Macromolecules crowd defined spaces, thereby excluding other like-sized molecules from the volume they occupy. These excluded-volume effect(s) (EVE) are well characterized for intracellular and partially for extracellular compartments such as blood plasma. We showed that EVE in fibroblast culture leads to faster enzymatic procollagen conversion and matrix deposition. Apparently, EVE can be applied to emulate in vivo conditions in an in vitro setting. Thus, we attempted to quantitatively capture the crowding potential of various macromolecules using dynamic light scattering under physiological conditions. We found that charged macromolecules like dextran sulfate (negative, 500 kDa) have a hydrodynamic radii of 46.4 ± 0.3 nm i.e. ~4 fold larger than that of neutral macromolecules like Ficoll (neutral, 400 kDa) and thus show greater EVE potential. At biologically effective concentrations viscosity was not increased. Unexpectedly, we observed a dramatic drop of hydrodynamic radii of all macromolecules tested above a threshold concentration. This suggested a hyper-crowding state in which the crowders compacted themselves mutually. We will use this hyper-crowding threshold to determine retrogradely rules that allow to predict the conditions for optimum crowding effects (such as the half-hyper-crowding concentration) in biological systems. We propose Dynamic Light Scattering (DLS) as a potential tool to estimate EVE in biotechnical applications.

An excluded volume effect in light scattering

1958 ◽  
Vol 33 (126) ◽  
pp. 129-140 ◽  
Author(s):  
A. J. Hyde ◽  
J. H. Ryan ◽  
F. T. Wall ◽  
T. F. Schatzki
Keyword(s):  
Light Scattering ◽  
Volume Effect ◽  
Excluded Volume ◽  
Excluded Volume Effect

scholarly journals Light Scattering Study on the Structure of Pure Poly(methyl methacrylate)

1983 ◽  
Vol 15 (10) ◽  
pp. 693-698 ◽  
Author(s):  
Michiya Fujiki ◽  
Toshikuni Kaino ◽  
Shigeru Oikawa
Keyword(s):  
Light Scattering ◽  
Methyl Methacrylate ◽  
Poly Methyl Methacrylate ◽  
Scattering Study ◽  
Pure Poly
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