Electrophoretic Light Scattering, Dynamic Light Scattering, and Turbidimetry Studies of the Effect of Polymer Concentration on Complex Formation between Polyelectrolyte and Oppositely Charged Mixed Micelles

1995 ◽  
Vol 28 (9) ◽  
pp. 3098-3102 ◽  
Author(s):  
Yingjie Li ◽  
Paul L. Dubin ◽  
Henry A. Havel ◽  
Shun L. Edwards ◽  
Herbert Dautzenberg
Keyword(s):  
Light Scattering ◽  
Complex Formation ◽  
Dynamic Light Scattering ◽  
Mixed Micelles ◽  
Polymer Concentration ◽  
Electrophoretic Light Scattering ◽  
Oppositely Charged

Self-Aggregation of Mixtures of Oppositely Charged Polyelectrolytes and Surfactants Studied by Rheology, Dynamic Light Scattering and Small-Angle Neutron Scattering

Langmuir ◽  
2011 ◽  
Vol 27 (8) ◽  
pp. 4386-4396 ◽  
Author(s):  
Ingo Hoffmann ◽  
Peggy Heunemann ◽  
Sylvain Prévost ◽  
Ralf Schweins ◽  
Norman J. Wagner ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Neutron Scattering ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Oppositely Charged ◽  
Self Aggregation

scholarly journals Effect of the Dispersion State in Y5O4F7 Suspension on YOF Coating Deposited by Suspension Plasma Spray

Coatings ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 831
Author(s):  
Seungjun Lee ◽  
Jaehoo Lee ◽  
Nongmoon Hwang
Keyword(s):  
Particle Size ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Zeta Potential ◽  
Average Particle Size ◽  
Average Particle ◽  
Electrophoretic Light Scattering ◽  
Electrosteric Stabilization ◽  
Dispersion State ◽  
Yttrium Oxyfluoride

The stable Y5O4F7 suspension for dense yttrium oxyfluoride (YOF) coating by suspension plasma spraying (SPS) was developed. Electrostatically and electrosterically stabilized aqueous Y5O4F7 suspensions were prepared and compared with a commercially available Y5O4F7 suspension without dispersant. The wettability and dispersibility of the Y5O4F7 suspensions were evaluated in terms of the zeta potential, average particle size, and size distribution with electrophoretic light scattering (ELS) and dynamic light scattering (DLS). The viscosity was measured and the sedimentation was tested to examine the fluidity and stability of the Y5O4F7 suspensions. When electrostatic (BYK-154) and electrosteric (BYK-199) dispersants were added to the Y5O4F7 suspension, the isoelectric point (IEP) of Y5O4F7 particles in the suspension shifted to lower pH. The zeta potential of both of electrostatically and electrosterically stabilized Y5O4F7 suspensions were higher than ±40 mV at pH of 8.6, respectively, which were much higher than of the Y5O4F7 suspension without dispersant. Meanwhile, the average particle size of the electrosterically stabilized Y5O4F7 suspension was much smaller than that of the electrostatically stabilized one. The electrosteric stabilization had a great effect on improving the wettability and dispersibility of the Y5O4F7 suspension. The coating rate of the electrosterically stabilized Y5O4F7 suspension was the highest among the three tested suspensions. In addition, the YOF coating deposited with the electrosterically stabilized Y5O4F7 suspension had the highest hardness and the lowest porosity.

The oligomeric state, complex formation, and chaperoning activity of Hsp70 and Hsp80 of Neurospora crassa

2002 ◽  
Vol 80 (6) ◽  
pp. 797-809 ◽  
Author(s):  
M E Britton ◽  
M Kapoor
Keyword(s):  
Surface Plasmon Resonance ◽  
Light Scattering ◽  
Complex Formation ◽  
Dynamic Light Scattering ◽  
Neurospora Crassa ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Molecular Chaperones ◽  
Citrate Synthase ◽  
Oligomeric State

Molecular chaperones perform vital cellular functions under normal growth conditions and protect cells against stress-induced damage. The stress proteins Hsp70 and Hsp80 of Neurospora crassa were extracted from heat-shocked mycelium, purified to near homogeneity, and examined with respect to their oligomeric state, complex formation, and chaperoning properties. Their oligomeric state was assessed by dynamic light-scattering measurements, and both Hsp70 and Hsp80 were observed to form a range of soluble, high-molecular-mass protein aggregates. Direct interaction between Hsp70 and Hsp80 was studied by partial tryptic digestion and surface plasmon resonance (SPR). Hsp70 was immobilized on the sensor chip surface, and the binding of Hsp80 in solution was followed in real time. Proteolytic digestion revealed that Hsp70–Hsp80 complex formation results in conformational changes in both proteins. The data from SPR studies yielded an equilibrium dissociation constant, KD, of 8.5 × 10–9 M. The chaperoning ability of Hsp70, Hsp80, and Hsp70–Hsp80 was monitored in vitro by the protection of citrate synthase from thermal aggregation. The binding of nucleotides modulates the oligomeric state, chaperoning function, and hetero-oligomeric complex formation of Hsp70 and Hsp80.Key words: molecular chaperones, surface plasmon resonance, dynamic light scattering, trypsin digestion, citrate synthase, Neurospora crassa.

Characterization of sodium dodecylsulphate and dodecylphosphocholine mixed micelles through NMR and dynamic light scattering

2013 ◽  
Vol 51 (3) ◽  
pp. 176-183 ◽  
Author(s):  
Giorgia Manzo ◽  
Maura Carboni ◽  
Andrea C. Rinaldi ◽  
Mariano Casu ◽  
Mariano A. Scorciapino
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Mixed Micelles ◽  
Sodium Dodecylsulphate

scholarly journals Heteroaggregation and Homoaggregation of Latex Particles in the Presence of Alkyl Sulfate Surfactants

2020 ◽  
Vol 4 (4) ◽  
pp. 52
Author(s):  
Tianchi Cao ◽  
Michal Borkovec ◽  
Gregor Trefalt
Keyword(s):  
Light Scattering ◽  
Sodium Dodecyl Sulfate ◽  
Dynamic Light Scattering ◽  
Isoelectric Point ◽  
Sodium Dodecyl ◽  
Charge Reversal ◽  
Time Resolved ◽  
Latex Particles ◽  
A Charge ◽  
Oppositely Charged

Heteroaggregation and homoaggregation is investigated with time-resolved multi-angle dynamic light scattering. The aggregation rates are measured in aqueous suspensions of amidine latex (AL) and sulfate latex (SL) particles in the presence of sodium octyl sulfate (SOS) and sodium dodecyl sulfate (SDS). As revealed by electrophoresis, the surfactants adsorb to both types of particles. For the AL particles, the adsorption of surfactants induces a charge reversal and triggers fast aggregation close to the isoelectric point (IEP). The negatively charged SL particles remain negatively charged and stable in the whole concentration range investigated. The heteroaggregation rates for AL and SL particles are fast at low surfactant concentrations, where the particles are oppositely charged. At higher concentrations, the heteroaggregation slows down above the IEP of the AL particles, where the particles become like-charged. The SDS has higher affinity to the surface compared to the SOS, which induces a shift of the IEP and of the fast aggregation regime to lower surfactant concentrations.

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