Formation of Supported Phospholipid Bilayers from Unilamellar Vesicles Investigated by Atomic Force Microscopy

Langmuir ◽  
2000 ◽  
Vol 16 (4) ◽  
pp. 1806-1815 ◽  
Author(s):  
Ilya Reviakine ◽  
Alain Brisson
Keyword(s):  
Atomic Force Microscopy ◽  
Phospholipid Bilayers ◽  
Unilamellar Vesicles ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Enzyme-catalyzed hydrolysis of the supported phospholipid bilayers studied by atomic force microscopy

2013 ◽  
Vol 1828 (2) ◽  
pp. 642-651 ◽  
Author(s):  
HengLiang Wu ◽  
Le Yu ◽  
Yujin Tong ◽  
Aimin Ge ◽  
Shuehlin Yau ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Phospholipid Bilayers ◽  
Force Microscopy ◽  
Atomic Force ◽  
Hydrolysis Of

scholarly journals Atomic force microscopy of phase separation on ruptured, giant unilamellar vesicles

2018 ◽  
Author(s):  
Yanfei Jiang ◽  
Guy M. Genin ◽  
Kenneth M. Pryse ◽  
Elliot L. Elson
Keyword(s):  
Atomic Force Microscopy ◽  
Phase Separation ◽  
Model Systems ◽  
Study Phase ◽  
Dark Phase ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Force Microscopy ◽  
Atomic Force ◽  
Lipid Species

AbstractGiant unilamellar vesicles (GUVs) and supported lipid bilayers (SLBs) are synthetic model systems widely used in biophysical studies of lipid membranes. Phase separation behaviors of lipid species in these two model systems differ due to the lipid-substrate interactions that are present only for SLBs. Therefore, GUVs are believed to resemble natural cell membranes more closely, and a very large body of literature focuses on applying nano-characterization techniques to quantify phase separation on GUVs. However, one important technique, atomic force microscopy (AFM), has not yet been used successfully to study phase separation on GUVs. In the present study, we report that in binary systems, certain phase domains on GUVs retain their original shapes and patterns after the GUVs rupture on glass surfaces. This enabled AFM experiments on phase domains from binary GUVs containing 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) and either 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) or 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC). These DLPC/DSPC and DLPC/DPPC GUVs both presented two different gel phases, one of which (bright phase) included a relatively high concentration of DiI-C20 but excluded Bodipy-HPC, and the other of which (dark phase) excluded both probes. The bright phases are of interest because they seem to stabilize dark phases against coalescence. Results suggested that the gel phases labeled by DiI-C20 in the DLPC/DSPC membrane, which surround the dark gel phase, is an extra layer of membrane, indicating a highly curved structure that might stabilize the interior dark domains. This phenomenon was not found in the DLPC/DPPC membrane. These results show the utility of AFM on collapsed GUVs, and suggest a possible mechanism for stabilization of lipid domains.

scholarly journals Filipin-Induced Lesions in Planar Phospholipid Bilayers Imaged by Atomic Force Microscopy

1998 ◽  
Vol 75 (4) ◽  
pp. 1869-1873 ◽  
Author(s):  
Nuno C. Santos ◽  
Evgeny Ter-Ovanesyan ◽  
Joseph A. Zasadzinski ◽  
Manuel Prieto ◽  
Miguel A.R.B. Castanho
Keyword(s):  
Atomic Force Microscopy ◽  
Phospholipid Bilayers ◽  
Force Microscopy ◽  
Atomic Force

Photolithographic Polymerization of Diacetylene-Containing Phospholipid Bilayers Studied by Multimode Atomic Force Microscopy

Langmuir ◽  
2003 ◽  
Vol 19 (17) ◽  
pp. 6994-7002 ◽  
Author(s):  
Kenichi Morigaki ◽  
Holger Schönherr ◽  
Curtis W. Frank ◽  
Wolfgang Knoll
Keyword(s):  
Atomic Force Microscopy ◽  
Phospholipid Bilayers ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Atomic Force Microscopy of Phase Separation on Ruptured, Giant Unilamellar Vesicles, and a Mechanical Pathway for the Co-Existence of Lipid Gel Phases

2019 ◽  
Vol 141 (7) ◽  
Author(s):  
Yanfei Jiang ◽  
Kenneth M. Pryse ◽  
Srikanth Singamaneni ◽  
Guy M. Genin ◽  
Elliot L. Elson
Keyword(s):  
Atomic Force Microscopy ◽  
Phase Separation ◽  
Lipid Vesicles ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Surface Receptors ◽  
Force Microscopy ◽  
Atomic Force ◽  
Lipid Species ◽  
Surface Domains

Phase separation of lipid species is believed to underlie formation of lipid rafts that enable the concentration of certain surface receptors. However, the dynamics and stabilization of the resulting surface domains are unclear. We developed a methodology for collapsing giant unilamellar vesicles (GUVs) into supported bilayers in a way that keeps membrane nanodomains stable and enables their imaging. We used a combination of fluorescence and atomic force microscopy (AFM) of this system to uncover how a surprising phase separation occurs on lipid vesicles, in which two different gel phases of the same lipid co-exist. This unusual phase behavior was evident in binary GUVs containing 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) and either 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) or 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC). The approach showed that one of the phases is stabilized by lipid patches that become ejected from the membrane, thereby enabling the stabilization of what would otherwise be a thermodynamically impossible coexistence. These results show the utility of AFM on collapsed GUVs, and suggest a possible mechanical mechanism for stabilization of lipid domains.

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