scholarly journals Using “Click” Chemistry to Prepare SAM Substrates to Study Stem Cell Adhesion

Langmuir ◽  
2009 ◽  
Vol 25 (10) ◽  
pp. 5737-5746 ◽  
Author(s):  
Gregory A. Hudalla ◽  
William L. Murphy
Keyword(s):  
Stem Cell ◽  
Cell Adhesion ◽  
Click Chemistry

Changing ligand number and type within nanocylindrical domains through kinetically constrained self-assembly – impacts of ligand ‘redundancy’ on human mesenchymal stem cell adhesion and morphology

2014 ◽  
Vol 2 (11) ◽  
pp. 1693-1705 ◽  
Author(s):  
Haiqing Li ◽  
Justin J. Cooper-White
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Cell Adhesion ◽  
Block Copolymer ◽  
Mesenchymal Stem Cell ◽  
Click Chemistry ◽  
Self Assembly ◽  
Human Mesenchymal Stem Cell ◽  
Culture Surface ◽  
Self Assembled

In this paper, we firstly describe a facile method by which sequential attachment of different adhesion peptides to a nanotopographical, self-assembled block copolymer cell culture surface is made possible through orthogonal click chemistry.

scholarly journals Label-free Imaging of Stem Cell Adhesion and Dynamic Tracking of Boundary Evolution Using Photonic Crystal Enhanced Microscopy (PCEM)

2017 ◽  
Vol 23 (S1) ◽  
pp. 1142-1143 ◽  
Author(s):  
Yue Zhuo ◽  
Ji Sun Choi ◽  
Thibault Marin ◽  
Hojeong Yu ◽  
Brendan A. Harley ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Adhesion ◽  
Photonic Crystal ◽  
Label Free ◽  
Dynamic Tracking

scholarly journals Persistence of intramyocardially transplanted murine induced pluripotent stem cell-derived cardiomyocytes from different developmental stages

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Gabriel Peinkofer ◽  
Martina Maass ◽  
Kurt Pfannkuche ◽  
Agapios Sachinidis ◽  
Stephan Baldus ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Cell Adhesion ◽  
Developmental Stage ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Induced Pluripotent

Abstract Background Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM) are regarded as promising cell type for cardiac cell replacement therapy, but it is not known whether the developmental stage influences their persistence and functional integration in the host tissue, which are crucial for a long-term therapeutic benefit. To investigate this, we first tested the cell adhesion capability of murine iPSC-CM in vitro at three different time points during the differentiation process and then examined cell persistence and quality of electrical integration in the infarcted myocardium in vivo. Methods To test cell adhesion capabilities in vitro, iPSC-CM were seeded on fibronectin-coated cell culture dishes and decellularized ventricular extracellular matrix (ECM) scaffolds. After fixed periods of time, stably attached cells were quantified. For in vivo experiments, murine iPSC-CM expressing enhanced green fluorescent protein was injected into infarcted hearts of adult mice. After 6–7 days, viable ventricular tissue slices were prepared to enable action potential (AP) recordings in transplanted iPSC-CM and surrounding host cardiomyocytes. Afterwards, slices were lysed, and genomic DNA was prepared, which was then used for quantitative real-time PCR to evaluate grafted iPSC-CM count. Results The in vitro results indicated differences in cell adhesion capabilities between day 14, day 16, and day 18 iPSC-CM with day 14 iPSC-CM showing the largest number of attached cells on ECM scaffolds. After intramyocardial injection, day 14 iPSC-CM showed a significant higher cell count compared to day 16 iPSC-CM. AP measurements revealed no significant difference in the quality of electrical integration and only minor differences in AP properties between d14 and d16 iPSC-CM. Conclusion The results of the present study demonstrate that the developmental stage at the time of transplantation is crucial for the persistence of transplanted iPSC-CM. iPSC-CM at day 14 of differentiation showed the highest persistence after transplantation in vivo, which may be explained by a higher capability to adhere to the extracellular matrix.

scholarly journals Organizational metrics of interchromatin speckle factor domains: integrative classifier for stem cell adhesion & lineage signaling

2015 ◽  
Vol 7 (4) ◽  
pp. 435-446 ◽  
Author(s):  
Sebastián L. Vega ◽  
Anandika Dhaliwal ◽  
Varun Arvind ◽  
Parth J. Patel ◽  
Nick R. M. Beijer ◽  
...  
Keyword(s):  
Content Analysis ◽  
Stem Cell ◽  
Cell Adhesion ◽  
Nuclear Protein ◽  
Cell Lineage ◽  
Lineage Commitment ◽  
Cell Microenvironment ◽  
Stem Cell Lineage ◽  
High Content Analysis

Timely classification of stem cell lineage commitment in response to cell–microenvironment interactions using high content analysis of sub-nuclear protein organization.

scholarly journals Surface functionalisation of nanodiamonds for human neural stem cell adhesion and proliferation

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Alice C. Taylor ◽  
Citlali Helenes González ◽  
Benjamin S. Miller ◽  
Robert J. Edgington ◽  
Patrizia Ferretti ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Adhesion ◽  
Neural Stem Cell ◽  
Human Neural Stem Cell ◽  
Surface Functionalisation ◽  
Cell Adhesion And Proliferation

scholarly journals Development of a Dynamic Stem Cell Culture Platform for Mesenchymal Stem Cell Adhesion and Evaluation

2014 ◽  
Vol 11 (7) ◽  
pp. 2172-2181 ◽  
Author(s):  
Kimberly M. Ferlin ◽  
Margaret E. Prendergast ◽  
Makenzie L. Miller ◽  
Bao-Ngoc B. Nguyen ◽  
David S. Kaplan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Cell Adhesion ◽  
Mesenchymal Stem Cell ◽  
Stem Cell Culture
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