scholarly journals Measuring Diffusion of Lipid-like Probes in Artificial and Natural Membranes by Raster Image Correlation Spectroscopy (RICS): Use of a Commercial Laser-Scanning Microscope with Analog Detection

Langmuir ◽  
2009 ◽  
Vol 25 (9) ◽  
pp. 5209-5218 ◽  
Author(s):  
Ellen Gielen ◽  
Nick Smisdom ◽  
Martin vandeVen ◽  
Ben De Clercq ◽  
Enrico Gratton ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Scanning Microscope ◽  
Laser Scanning Microscope ◽  
Raster Image ◽  
Image Correlation Spectroscopy

Determination of Dynamics of Plant Plasma Membrane Proteins with Fluorescence Recovery and Raster Image Correlation Spectroscopy

2016 ◽  
Vol 22 (2) ◽  
pp. 290-299 ◽  
Author(s):  
Martina Laňková ◽  
Jana Humpolíčková ◽  
Stanislav Vosolsobě ◽  
Zdeněk Cit ◽  
Jozef Lacek ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Laser Scanning ◽  
Correlation Spectroscopy ◽  
Laser Scanning Microscopy ◽  
Image Correlation ◽  
Fluorescence Recovery ◽  
Confocal Laser ◽  
Raster Image ◽  
Image Correlation Spectroscopy ◽  
Plant Plasma Membrane

AbstractA number of fluorescence microscopy techniques are described to study dynamics of fluorescently labeled proteins, lipids, nucleic acids, and whole organelles. However, for studies of plant plasma membrane (PM) proteins, the number of these techniques is still limited because of the high complexity of processes that determine the dynamics of PM proteins and the existence of cell wall. Here, we report on the usage of raster image correlation spectroscopy (RICS) for studies of integral PM proteins in suspension-cultured tobacco cells and show its potential in comparison with the more widely used fluorescence recovery after photobleaching method. For RICS, a set of microscopy images is obtained by single-photon confocal laser scanning microscopy (CLSM). Fluorescence fluctuations are subsequently correlated between individual pixels and the information on protein mobility are extracted using a model that considers processes generating the fluctuations such as diffusion and chemical binding reactions. As we show here using an example of two integral PM transporters of the plant hormone auxin, RICS uncovered their distinct short-distance lateral mobility within the PM that is dependent on cytoskeleton and sterol composition of the PM. RICS, which is routinely accessible on modern CLSM instruments, thus represents a valuable approach for studies of dynamics of PM proteins in plants.

Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds

2011 ◽  
Vol 7 (12) ◽  
pp. 4195-4203 ◽  
Author(s):  
S.C.P. Norris ◽  
J. Humpolíčková ◽  
E. Amler ◽  
M. Huranová ◽  
M. Buzgo ◽  
...  
Keyword(s):  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Raster Image ◽  
Nanofiber Scaffolds ◽  
Image Correlation Spectroscopy

scholarly journals Measuring Lateral Diffusion of Receptors On Plasma Membrane of Macrophages Using Raster Image Correlation Spectroscopy

2018 ◽  
Vol 24 (S1) ◽  
pp. 1356-1357
Author(s):  
S. Makaremi ◽  
S. Ranjit ◽  
M.A. Digman ◽  
E. Gratton ◽  
D. M.E. Bowdish ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Lateral Diffusion ◽  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Raster Image ◽  
Image Correlation Spectroscopy

scholarly journals Measurement of Protein 53 Diffusion Coefficient in Live HeLa Cells Using Raster Image Correlation Spectroscopy (RICS)

2010 ◽  
Vol 01 (01) ◽  
pp. 31-36 ◽  
Author(s):  
Sungmin Hong ◽  
Ying-Nai Wang ◽  
Hirohito Yamaguchi ◽  
Harinibytaraya Sreenivasappa ◽  
Chao-Kai Chou ◽  
...  
Keyword(s):  
Diffusion Coefficient ◽  
Hela Cells ◽  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Raster Image ◽  
Image Correlation Spectroscopy

scholarly journals Raster Image Correlation Spectroscopy Performance Evaluation

2019 ◽  
Vol 117 (10) ◽  
pp. 1900-1914 ◽  
Author(s):  
Marco Longfils ◽  
Nick Smisdom ◽  
Marcel Ameloot ◽  
Mats Rudemo ◽  
Veerle Lemmens ◽  
...  
Keyword(s):  
Performance Evaluation ◽  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Raster Image ◽  
Image Correlation Spectroscopy

Dynamic range and background filtering in raster image correlation spectroscopy

2020 ◽  
Vol 279 (2) ◽  
pp. 123-138
Author(s):  
R. DE METS ◽  
A. DELON ◽  
M. BALLAND ◽  
O. DESTAING ◽  
I. WANG
Keyword(s):  
Dynamic Range ◽  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Raster Image ◽  
Image Correlation Spectroscopy

scholarly journals Arbitrary-Region Raster Image Correlation Spectroscopy

2016 ◽  
Vol 111 (8) ◽  
pp. 1785-1796 ◽  
Author(s):  
Jelle Hendrix ◽  
Tomas Dekens ◽  
Waldemar Schrimpf ◽  
Don C. Lamb
Keyword(s):  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Arbitrary Region ◽  
Raster Image ◽  
Image Correlation Spectroscopy
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