Renewable and Optically Transparent Electroactive Indium Tin Oxide Surfaces for Chemoselective Ligand Immobilization and Biospecific Cell Adhesion

Langmuir ◽  
2008 ◽  
Vol 24 (22) ◽  
pp. 13096-13101 ◽  
Author(s):  
Wei Luo ◽  
Nathan P. Westcott ◽  
Abigail Pulsipher ◽  
Muhammad N. Yousaf
Keyword(s):  
Cell Adhesion ◽  
Tin Oxide ◽  
Indium Tin Oxide ◽  
Oxide Surfaces ◽  
Optically Transparent

Development of a carboxyl-terminated Indium Tin Oxide electrode for improving cell adhesion and facilitating low noise, real-time impedance measurements

2021 ◽  
Author(s):  
A.M. Ilyas ◽  
Md Kowsar Alam ◽  
Jamal-Deen Musah ◽  
Lin Oo SAW ◽  
Chi-Chung Yeung ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Tin Oxide ◽  
Indium Tin Oxide ◽  
Time Course ◽  
Low Noise ◽  
Collective Cell Migration ◽  
Signal Loss ◽  
Ito Cell ◽  
Cell Substrate ◽  
Multiple Cells

The working electrode's surface property is crucial to cell adhesion and signal collection in electric cell-substrate impedance spectroscopy (ECIS). To date, the indium tin oxide (ITO) based working electrode is of interest in ECIS study due to its high transparency and biocompatibility. Of great concern is the impedance signal loss, distortion, and data interpretation conflict profoundly created by the movement of multiple cells during ECIS study. Here, a carboxyl- terminated-ITO substrate was prepared by stepwise surface amino silanization, with N-Hydroxy succinimide (NHS), and (1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride) (EDC) treatment, respectively. We investigated the stepwise changes in the property of the treated ITO, cell-substrate adhesion, collective cell mobility, and time course of change in absolute impedance from multiple CHO cells ((Δt-Δ|Z|)CELLS). The carboxyl-terminated ITO substrate with a surface roughness of 6.37 nm shows enhanced conductivity, 75% visible light transparency, improved cell adherence, reduced collective cell migration speed by ~2 fold, and diminished signal distortion in the ((Δt-Δ|Z|)CELLS). Thus, our study provides an ITO surface-treatment strategy to reduce multiple cell movement effects and to obtain essential cell information from the ECIS study of multiple cells through undistorted (Δt-Δ|Z|)CELLS

Anchoring of Self-Assembled Hemoglobin Molecules on Bare Indium−Tin Oxide Surfaces

Langmuir ◽  
2001 ◽  
Vol 17 (14) ◽  
pp. 4360-4366 ◽  
Author(s):  
Aiping Fang ◽  
HouTee Ng ◽  
Sam Fong Yau Li
Keyword(s):  
Tin Oxide ◽  
Indium Tin Oxide ◽  
Oxide Surfaces ◽  
Self Assembled

Preparation and microstructural characterization of oriented titanosilicate ETS-10 thin films on indium tin oxide surfaces

2010 ◽  
Vol 131 (1-3) ◽  
pp. 401-406 ◽  
Author(s):  
Sezin Galioğlu ◽  
Mariam N. Ismail ◽  
Juliusz Warzywoda ◽  
Albert Sacco ◽  
Burcu Akata
Keyword(s):  
Thin Films ◽  
Tin Oxide ◽  
Indium Tin Oxide ◽  
Microstructural Characterization ◽  
Oxide Surfaces
Sign in / Sign up

Export Citation Format

Share Document