Preparation of Well-Defined Core−Shell Particles by Cu2+-Mediated Graft Copolymerization of Methyl Methacrylate from Bovine Serum Albumin

Langmuir ◽  
2008 ◽  
Vol 24 (19) ◽  
pp. 10717-10722 ◽  
Author(s):  
Chuanxin He ◽  
Jianhong Liu ◽  
Xiaodong Ye ◽  
Laiyong Xie ◽  
Qianling Zhang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Methyl Methacrylate ◽  
Bovine Serum ◽  
Graft Copolymerization ◽  
Core Shell ◽  
Shell Particles

Bovine Serum Albumin Conjugation in Superparamagnetic/Poly(methyl methacrylate) Nanoparticles as an Alternative for Magnetic Enzyme-Linked Immunosorbent Assays

2021 ◽  
Vol 21 (11) ◽  
pp. 5493-5498
Author(s):  
Paulo Emilio Feuser ◽  
Camila Guindani ◽  
Jonathann Correa Possato ◽  
Jaqueline Pereira Guessi ◽  
Arthur Poester Cordeiro ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Methyl Methacrylate ◽  
Bovine Serum ◽  
Protein Quantification ◽  
Elisa Assay ◽  
Poly Methyl Methacrylate ◽  
Transmission Electron ◽  
Superparamagnetic Properties ◽  
Significant Attention

Nanomaterials, such as magnetic nanoparticles have attracted significant attention of medical area due to their capacity to improve the performance of immunoassays. Therefore the aim of this work was to study the bovine serum albumin (BSA) conjugation in superparamagnetic (MNPs)/poly(methyl methacrylate) (PMMA) nanoparticles with further characterization and application in enzyme-linked immunosorbent (ELISA) assay. The successful conjugation of BSA in MNPs- PMMA nanoparticles was confirmed by several techniques, including light scattering, zeta potential, transmission electron microscopy (TEM) and Lowry protein quantification assay. The superparamagnetic properties were confirmed by vibrating sample magnetometer. BSA conjugated MNPs-PMMA nanoparticles presented higher interactions with antibody than free BSA. The BSA + MNPs-PMMA nanoparticles (magnetic ELISA assay) reduced the time and increased the sensibility of traditional ELISA assay, reinforcing the idea that the use these nanomaterials are an excellent alternative for the immunoassays field.

Study of the interaction between CdSe/ZnS core-shell quantum dots and bovine serum albumin by spectroscopic techniques

2010 ◽  
Vol 215 (1) ◽  
pp. 118-122 ◽  
Author(s):  
Milohum Mikesokpo Dzagli ◽  
Valentin Canpean ◽  
Monica Iosin ◽  
Messanh Agbeko Mohou ◽  
Simion Astilean
Keyword(s):  
Quantum Dots ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Core Shell ◽  
Spectroscopic Techniques
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