Colloidal Deposition on Remotely Controlled Charged Micropatterned Surfaces in a Parallel-Plate Flow Chamber

Langmuir ◽  
2008 ◽  
Vol 24 (17) ◽  
pp. 9381-9385 ◽  
Author(s):  
Timothy R. Kline ◽  
Gexin Chen ◽  
Sharon L. Walker
Keyword(s):  
Parallel Plate ◽  
Flow Chamber ◽  
Parallel Plate Flow Chamber ◽  
Colloidal Deposition

VCAM-1 is more effective than MAdCAM-1 in supporting eosinophil rolling under conditions of shear flow

Blood ◽  
2000 ◽  
Vol 95 (2) ◽  
pp. 592-601 ◽  
Author(s):  
P. Sriramarao ◽  
Richard G. DiScipio ◽  
Ronald R. Cobb ◽  
Myron Cybulsky ◽  
Greg Stachnick ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Parallel Plate ◽  
Cell Adhesion Molecule ◽  
Flow Chamber ◽  
Β1 Integrin ◽  
Vascular Cell Adhesion ◽  
Parallel Plate Flow Chamber ◽  
Vascular Cell

The ability of the 4 integrin counterligands vascular cell adhesion molecule (VCAM)-1 or mucosal addressin (MAd)CAM-1 to support eosinophil rolling or firm adhesion under conditions of physiologic flow has not been delineated. Using a parallel plate flow chamber in vitro and intravital microscopy in vivo, we demonstrate that eosinophil rolling and adhesion on VCAM-1 is mediated by both 4β1 and 4β7 integrins. Eosinophils rolled equally efficiently on both VCAM-1 2 domain and VCAM-1 7 domain, suggesting that the N-terminal 2 domains of VCAM-1 are sufficient to support eosinophil rolling under conditions of flow. Furthermore, activation of the eosinophil β1 integrin with monoclonal antibody (mAb) 8A2 resulted in both resistance to shear stress–induced detachment from VCAM-1 in vitro and in stable arrest of rolling eosinophils on interleukin (IL)-1β–stimulated venules in vivo. Eosinophils rolled less efficiently on MAdCAM-1– than on VCAM-1–coated coverslips under conditions of flow. However, eosinophils firmly adhered as efficiently to MAdCAM-1 as to VCAM-1. Overall, these results demonstrate that both VCAM-1 and MAdCAM-1 can support eosinophil firm adhesion under conditions of flow. In contrast, VCAM-1 is significantly more efficient than MAdCAM-1 in supporting eosinophil rolling under conditions of flow.

Wall Shear Stress Determination in a Small-Scale Parallel Plate Flow Chamber Using Laser Doppler Velocimetry Under Laminar, Pulsatile and Low-Reynolds Number Turbulent Flows

2018 ◽  
Vol 140 (6) ◽  
Author(s):  
Hamed Avari ◽  
Kem A. Rogers ◽  
Eric Savory
Keyword(s):  
Shear Stress ◽  
Wall Shear Stress ◽  
Laser Doppler Velocimetry ◽  
Parallel Plate ◽  
Flow Chamber ◽  
Laser Doppler ◽  
Small Scale ◽  
Doppler Velocimetry ◽  
Flow Conditions ◽  
Parallel Plate Flow Chamber

The parallel plate flow chamber (PPFC) has gained popularity due to its applications in fields such as biological tissue engineering. However, most of the studies using PPFC refer to theoretical relations for estimating the wall shear stress (WSS) and, hence, the accuracy of such quantifications remains elusive for anything other than steady laminar flow. In the current study, a laser Doppler velocimetry (LDV) method was used to quantify the flow in a PPFC (H = 1.8 mm × W = 17.5 mm, Dh = 3.26 mm, aspect ratio = 9.72) under steady Re = 990, laminar pulsatile (carotid Re0-mean = 282 as well as a non-zero-mean sinusoidal Re0-mean = 45 pulse) and low-Re turbulent Re = 2750 flow conditions. A mini-LDV probe was applied, and the absolute location of the LDV measuring volume with the respect to the wall was determined using a signal monitoring technique with uncertainties being around ±27 μm. The uniformity of the flow across the span of the channel, as well as the WSS assessment for all the flow conditions, was measured with the uncertainties all being less than 16%. At least two points within the viscous sublayer of the low-Re turbulent flow were measured (with the y+ for the first point < 3) and the WSS was determined using two methods with the differences between the two methods being within 5%. This paper for the first time presents the experimental determination of WSS using LDV in a small-scale PPFC under various flow conditions, the challenges associated with each condition, and a comparison between the cases. The present data will be useful for those conducting biological or numerical modeling studies using such devices.

Development and use of a parallel-plate flow chamber for studying cellular adhesion to solid surfaces

1992 ◽  
Vol 26 (6) ◽  
pp. 725-738 ◽  
Author(s):  
T. G. van Kooten ◽  
J. M. Schakenraad ◽  
H. C. Van der Mei ◽  
H. J. Busscher
Keyword(s):  
Parallel Plate ◽  
Flow Chamber ◽  
Cellular Adhesion ◽  
Solid Surfaces ◽  
Parallel Plate Flow Chamber
Sign in / Sign up

Export Citation Format

Share Document