Lipid Diffusion from Single Molecules of a Labeled Protein Undergoing Dynamic Association with Giant Unilamellar Vesicles and Supported Bilayers

Langmuir ◽  
2008 ◽  
Vol 24 (3) ◽  
pp. 844-850 ◽  
Author(s):  
Alexey Sharonov ◽  
Rakeshwar Bandichhor ◽  
Kevin Burgess ◽  
Anca D. Petrescu ◽  
Friedhelm Schroeder ◽  
...  
Keyword(s):  
Single Molecules ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Supported Bilayers ◽  
Dynamic Association ◽  
Lipid Diffusion

scholarly journals Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Naresh Yandrapalli ◽  
Julien Petit ◽  
Oliver Bäumchen ◽  
Tom Robinson
Keyword(s):  
High Throughput ◽  
Paradigm Shift ◽  
Mammalian Cells ◽  
Lipid Vesicles ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Artificial Cells ◽  
Membrane Function ◽  
Lipid Diffusion ◽  
Protein Incorporation

AbstractMicrofluidic production of giant lipid vesicles presents a paradigm-shift in the development of artificial cells. While production is high-throughput and the lipid vesicles are mono-disperse compared to bulk methods, current technologies rely heavily on the addition of additives such as surfactants, glycerol and even ethanol. Here we present a microfluidic method for producing biomimetic surfactant-free and additive-free giant unilamellar vesicles. The versatile design allows for the production of vesicle sizes ranging anywhere from ~10 to 130 µm with either neutral or charged lipids, and in physiological buffer conditions. Purity, functionality, and stability of the membranes are validated by lipid diffusion, protein incorporation, and leakage assays. Usability as artificial cells is demonstrated by increasing their complexity, i.e., by encapsulating plasmids, smaller liposomes, mammalian cells, and microspheres. This robust method capable of creating truly biomimetic artificial cells in high-throughput will prove valuable for bottom-up synthetic biology and the understanding of membrane function.

Lipid Diffusion in Giant Unilamellar Vesicles Is More than 2 Times Faster than in Supported Phospholipid Bilayers under Identical Conditions

Langmuir ◽  
2006 ◽  
Vol 22 (22) ◽  
pp. 9096-9099 ◽  
Author(s):  
Magdalena Przybylo ◽  
Jan Sýkora ◽  
Jana Humpolíčková ◽  
Aleš Benda ◽  
Anna Zan ◽  
...  
Keyword(s):  
Phospholipid Bilayers ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Lipid Diffusion

scholarly journals Effect of the Membrane Composition of Giant Unilamellar Vesicles on Their Budding Probability: A Trade-Off between Elasticity and Preferred Area Difference

Life ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 634
Author(s):  
Ylenia Miele ◽  
Gábor Holló ◽  
István Lagzi ◽  
Federico Rossi
Keyword(s):  
Phospholipid Fatty Acid ◽  
Enzymatic Reaction ◽  
Stimuli Responsive ◽  
Membrane Composition ◽  
Giant Unilamellar Vesicles ◽  
Physical Processes ◽  
Unilamellar Vesicles ◽  
Division Process ◽  
The Origin Of Life ◽  
Area Difference

The budding and division of artificial cells engineered from vesicles and droplets have gained much attention in the past few decades due to an increased interest in designing stimuli-responsive synthetic systems. Proper control of the division process is one of the main challenges in the field of synthetic biology and, especially in the context of the origin of life studies, it would be helpful to look for the simplest chemical and physical processes likely at play in prebiotic conditions. Here we show that pH-sensitive giant unilamellar vesicles composed of mixed phospholipid/fatty acid membranes undergo a budding process, internally fuelled by the urea–urease enzymatic reaction, only for a given range of the membrane composition. A gentle interplay between the effects of the membrane composition on the elasticity and the preferred area difference of the bilayer is responsible for the existence of a narrow range of membrane composition yielding a high probability for budding of the vesicles.

Domain Sorting in Giant Unilamellar Vesicles Adsorbed on Glass

Langmuir ◽  
2021 ◽  
Vol 37 (3) ◽  
pp. 1082-1088
Author(s):  
Chiho Kataoka-Hamai ◽  
Kohsaku Kawakami
Keyword(s):  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles

scholarly journals Production of giant unilamellar vesicles and encapsulation of lyotropic nematic liquid crystals

Soft Matter ◽  
2021 ◽  
Author(s):  
Peng Bao ◽  
Daniel A. Paterson ◽  
Sally A. Peyman ◽  
J. Cliff Jones ◽  
Jonathan A. T. Sandoe ◽  
...  
Keyword(s):  
Liquid Crystals ◽  
Nematic Liquid Crystals ◽  
Double Emulsion ◽  
Lyotropic Liquid Crystals ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Emulsion Droplets ◽  
Double Emulsion Droplets

We describe a modified microfluidic method for making Giant Unilamellar Vesicles (GUVs) via water/octanol-lipid/water double emulsion droplets and encapsulation of nematic lyotropic liquid crystals (LNLCs).

scholarly journals Inter- and Intra-Plane Interactions Control the Existence of Macrodomains in Asymmetric Giant Unilamellar Vesicles

2021 ◽  
Vol 120 (3) ◽  
pp. 147a
Author(s):  
Thais A. Enoki ◽  
Haden L. Scott ◽  
Gerald W. Feigenson ◽  
Frederick A. Heberle
Keyword(s):  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles

Ciprofloxacin Encapsulation Into Giant Unilamellar Vesicles: Membrane Binding and Release

2013 ◽  
Vol 102 (2) ◽  
pp. 694-705 ◽  
Author(s):  
Nóra Kaszás ◽  
Tamás Bozó ◽  
Marianna Budai ◽  
Pál Gróf
Keyword(s):  
Membrane Binding ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles
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