Gas-Phase Ion-Mobility Characterization of SAM-Functionalized Au Nanoparticles

Langmuir ◽  
2008 ◽  
Vol 24 (16) ◽  
pp. 8483-8490 ◽  
Author(s):  
D-H. Tsai ◽  
R. A. Zangmeister ◽  
L. F. Pease III ◽  
M. J. Tarlov ◽  
M. R. Zachariah
Keyword(s):  
Gas Phase ◽  
Ion Mobility ◽  
Au Nanoparticles ◽  
Gas Phase Ion

scholarly journals State-of-the-Art Native Mass Spectrometry and Ion Mobility Methods to Monitor Homogeneous Site-Specific Antibody-Drug Conjugates Synthesis

2021 ◽  
Vol 14 (6) ◽  
pp. 498
Author(s):  
Evolène Deslignière ◽  
Anthony Ehkirch ◽  
Bastiaan L. Duivelshof ◽  
Hanna Toftevall ◽  
Jonathan Sjögren ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Ion Mobility ◽  
State Of The Art ◽  
Native Mass Spectrometry ◽  
Site Specific ◽  
Drug Conjugates ◽  
Conjugation Process ◽  
Antibody Drug

Antibody-drug conjugates (ADCs) are biotherapeutics consisting of a tumor-targeting monoclonal antibody (mAb) linked covalently to a cytotoxic drug. Early generation ADCs were predominantly obtained through non-selective conjugation methods based on lysine and cysteine residues, resulting in heterogeneous populations with varying drug-to-antibody ratios (DAR). Site-specific conjugation is one of the current challenges in ADC development, allowing for controlled conjugation and production of homogeneous ADCs. We report here the characterization of a site-specific DAR2 ADC generated with the GlyCLICK three-step process, which involves glycan-based enzymatic remodeling and click chemistry, using state-of-the-art native mass spectrometry (nMS) methods. The conjugation process was monitored with size exclusion chromatography coupled to nMS (SEC-nMS), which offered a straightforward identification and quantification of all reaction products, providing a direct snapshot of the ADC homogeneity. Benefits of SEC-nMS were further demonstrated for forced degradation studies, for which fragments generated upon thermal stress were clearly identified, with no deconjugation of the drug linker observed for the T-GlyGLICK-DM1 ADC. Lastly, innovative ion mobility-based collision-induced unfolding (CIU) approaches were used to assess the gas-phase behavior of compounds along the conjugation process, highlighting an increased resistance of the mAb against gas-phase unfolding upon drug conjugation. Altogether, these state-of-the-art nMS methods represent innovative approaches to investigate drug loading and distribution of last generation ADCs, their evolution during the bioconjugation process and their impact on gas-phase stabilities. We envision nMS and CIU methods to improve the conformational characterization of next generation-empowered mAb-derived products such as engineered nanobodies, bispecific ADCs or immunocytokines.

scholarly journals Native Ion Mobility Mass Spectrometry: When Gas-Phase Ion Structures Depend on the Electrospray Charging Process

2019 ◽  
Vol 30 (6) ◽  
pp. 1069-1081 ◽  
Author(s):  
Nina Khristenko ◽  
Jussara Amato ◽  
Sandrine Livet ◽  
Bruno Pagano ◽  
Antonio Randazzo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry ◽  
Gas Phase Ion

Ab initiocalculation of the gas phase ion mobility of CO+ions in He

1999 ◽  
Vol 32 (20) ◽  
pp. 4947-4955 ◽  
Author(s):  
R G A R Maclagan ◽  
L A Viehland ◽  
A S Dickinson
Keyword(s):  
Gas Phase ◽  
Ion Mobility ◽  
Gas Phase Ion

Combining Structural Probes in the Gas Phase - Ion Mobility-Resolved Action-FRET

2017 ◽  
Vol 29 (1) ◽  
pp. 133-139 ◽  
Author(s):  
Steven Daly ◽  
Luke MacAleese ◽  
Philippe Dugourd ◽  
Fabien Chirot
Keyword(s):  
Gas Phase ◽  
Ion Mobility ◽  
Gas Phase Ion ◽  
Structural Probes

Rapid characterization of structure-dependency gas-phase ion/ion reaction via accumulative tandem MS

Talanta ◽  
2019 ◽  
Vol 195 ◽  
pp. 17-22 ◽  
Author(s):  
Muyi He ◽  
You Jiang ◽  
Xiaofeng Wang ◽  
Yue Zhao ◽  
Sijian Ye ◽  
...  
Keyword(s):  
Gas Phase ◽  
Tandem Ms ◽  
Rapid Characterization ◽  
Gas Phase Ion

Characterization of a DAPI-RIT-DAPI System for Gas-Phase Ion/Molecule and Ion/Ion Reactions

2013 ◽  
Vol 25 (1) ◽  
pp. 48-56 ◽  
Author(s):  
Ziqing Lin ◽  
Lei Tan ◽  
Sandilya Garimella ◽  
Linfan Li ◽  
Tsung-Chi Chen ◽  
...  
Keyword(s):  
Gas Phase ◽  
Gas Phase Ion
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