Photoinduced Increase in Vesicle Size and Role of Photoresponsive Malachite Green Leuconitrile Derivative in Vesicle Fusion

Langmuir ◽  
2007 ◽  
Vol 23 (15) ◽  
pp. 7936-7941 ◽  
Author(s):  
Ryoko M. Uda ◽  
Daisuke Yamashita ◽  
Yoshiaki Sakurai ◽  
Keiichi Kimura
Keyword(s):  
Malachite Green ◽  
Vesicle Fusion ◽  
Vesicle Size

scholarly journals Clathrin modulates vesicle scission, but not invagination shape, in yeast endocytosis

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Wanda Kukulski ◽  
Andrea Picco ◽  
Tanja Specht ◽  
John AG Briggs ◽  
Marko Kaksonen
Keyword(s):  
Particle Tracking ◽  
Light And Electron Microscopy ◽  
Tracking Method ◽  
Vesicle Size ◽  
Dynamic Architecture ◽  
Particle Tracking Method ◽  
Membrane Morphology ◽  
Membrane Invagination ◽  
Protein Components

In a previous paper (<xref ref-type="bibr" rid="bib22">Picco et al., 2015</xref>), the dynamic architecture of the protein machinery during clathrin-mediated endocytosis was visualized using a new live imaging and particle tracking method. Here, by combining this approach with correlative light and electron microscopy, we address the role of clathrin in this process. During endocytosis, clathrin forms a cage-like coat around the membrane and associated protein components. There is growing evidence that clathrin does not determine the membrane morphology of the invagination but rather modulates the progression of endocytosis. We investigate how the deletion of clathrin heavy chain impairs the dynamics and the morphology of the endocytic membrane in budding yeast. Our results show that clathrin is not required for elongating or shaping the endocytic membrane invagination. Instead, we find that clathrin contributes to the regularity of vesicle scission and thereby to controlling vesicle size.

scholarly journals The influence of malachite green on the level of transcriptional expression of the laccase and DyP-peroxidase genes of the Azospirillum brasilense

2020 ◽  
Author(s):  
M. A. Kupryashina ◽  
T. E. Pylaev ◽  
V. E. Nikitina
Keyword(s):  
Malachite Green ◽  
Azospirillum Brasilense ◽  
Expression Level ◽  
Synthetic Dyes ◽  
Transcriptional Expression ◽  
Bacterial Enzymes ◽  
Genes Expression

Malachite green (MG), a widely-used and recalcitrant dye, has been confirmed to be carcinogenic and mutagenic against many organisms. Herein, we were aimed at the investigation of the hypothetic role of ligninolytic bacterial enzymes similar to fungal ones in the degradation of synthetic dyes. A multiple increase in the laccases and DyP-peroxidases genes expression level was recorded by RT-qPCR for bacteria of the genus Azospirillum in the presence of MG.

scholarly journals GORASPs link Golgi cisternae laterally to stabilize the rims and prevent vesiculation

2020 ◽  
Author(s):  
Rianne Grond ◽  
Tineke Veenendaal ◽  
Juan Duran ◽  
Ishier Raote ◽  
Sebastiaan Corstjens ◽  
...  
Keyword(s):  
Dynamic Control ◽  
Vesicle Fusion ◽  
In Vitro Experiments ◽  
Cross Sectional ◽  
Data Support

AbstractIn vitro experiments have shown GRASP65 (GORASP1) and GRASP55 (GORASP2) proteins function in stacking Golgi cisternae. However, in vivo depletion of GORASPs in metazoans have given equivocal results. We have generated a mouse lacking both GORASPs and find that Golgi cisternae remained stacked. However, the stacks are disconnected laterally from each other and the cisternal cross-sectional diameters are significantly reduced compared to their normal counterparts. These data support earlier findings on the role of GORASPs in linking stacks and we suggest that unlinking of stacks affects dynamic control of COPI budding and vesicle fusion at the rims. The net result is that cisternal cores remain stacked, but cisternal diameter is reduced by rim consumption.

scholarly journals Postsynaptic regulation of synaptic plasticity by synaptotagmin 4 requires both C2 domains

2009 ◽  
Vol 187 (2) ◽  
pp. 295-310 ◽  
Author(s):  
Cynthia F. Barber ◽  
Ramon A. Jorquera ◽  
Jan E. Melom ◽  
J. Troy Littleton
Keyword(s):  
Synaptic Plasticity ◽  
Messenger Rna ◽  
Molecular Mechanisms ◽  
Neuromuscular Junctions ◽  
Vesicle Fusion ◽  
Synaptic Growth ◽  
Synaptotagmin 1 ◽  
Presynaptic Vesicle ◽  
Activity Dependent

Ca2+ influx into synaptic compartments during activity is a key mediator of neuronal plasticity. Although the role of presynaptic Ca2+ in triggering vesicle fusion though the Ca2+ sensor synaptotagmin 1 (Syt 1) is established, molecular mechanisms that underlie responses to postsynaptic Ca2+ influx remain unclear. In this study, we demonstrate that fusion-competent Syt 4 vesicles localize postsynaptically at both neuromuscular junctions (NMJs) and central nervous system synapses in Drosophila melanogaster. Syt 4 messenger RNA and protein expression are strongly regulated by neuronal activity, whereas altered levels of postsynaptic Syt 4 modify synaptic growth and presynaptic release properties. Syt 4 is required for known forms of activity-dependent structural plasticity at NMJs. Synaptic proliferation and retrograde signaling mediated by Syt 4 requires functional C2A and C2B Ca2+–binding sites, as well as serine 284, an evolutionarily conserved substitution for a key Ca2+-binding aspartic acid found in other synaptotagmins. These data suggest that Syt 4 regulates activity-dependent release of postsynaptic retrograde signals that promote synaptic plasticity, similar to the role of Syt 1 as a Ca2+ sensor for presynaptic vesicle fusion.

scholarly journals Reconstituting Intracellular Vesicle Fusion Reactions: The Essential Role of Macromolecular Crowding

2015 ◽  
Vol 137 (40) ◽  
pp. 12873-12883 ◽  
Author(s):  
Haijia Yu ◽  
Shailendra S. Rathore ◽  
Chong Shen ◽  
Yinghui Liu ◽  
Yan Ouyang ◽  
...  
Keyword(s):  
Essential Role ◽  
Macromolecular Crowding ◽  
Vesicle Fusion ◽  
Fusion Reactions ◽  
Intracellular Vesicle

scholarly journals Development of a Novel Ectonucleotidase Assay Suitable for High-Throughput Screening

2012 ◽  
Vol 17 (7) ◽  
pp. 993-998 ◽  
Author(s):  
Kris F. Sachsenmeier ◽  
Carl Hay ◽  
Erin Brand ◽  
Lori Clarke ◽  
Kim Rosenthal ◽  
...  
Keyword(s):  
Malachite Green ◽  
High Throughput ◽  
High Throughput Screening ◽  
High Performance ◽  
Adenosine Monophosphate ◽  
Screening Assay ◽  
Highly Sensitive ◽  
Development And Validation ◽  
Multiple Samples

5′-Ectonucleotidase (NT5E) catalyzes the conversion of adenosine monophosphate to adenosine and free phosphate. The role of this ectonucleotidase and its production of adenosine are linked with immune function, angiogenesis, and cancer. NT5E activity is typically assayed either by chromatographic quantification of substrates and products using high-performance liquid chromatography (HPLC) or by quantification of free phosphate using malachite green. These methods are not suitable for robust screening assays of NT5E activity. HPLC is not readily suitable for the rapid and efficient assay of multiple samples and malachite green is highly sensitive to the phosphate-containing buffers common in various media and sample buffers. Here the development and validation of a novel high-throughput ectonucleotidase screening assay are described, which makes use of a luciferase-based assay reagent, the Promega CellTiter-Glo kit, to measure the catabolism of AMP by NT5E. This multiwell plate-based assay facilitates the screening of potential ectonucleotidase antagonists and is unaffected by the presence of contaminating phosphate molecules present in screening samples.

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