scholarly journals In Situ Modulation of Cell Behavior via Smart Dual-Ligand Surfaces

Langmuir ◽  
2014 ◽  
Vol 30 (45) ◽  
pp. 13656-13666 ◽  
Author(s):  
Abigail Pulsipher ◽  
Sungjin Park ◽  
Debjit Dutta ◽  
Wei Luo ◽  
Muhammad N. Yousaf
Keyword(s):  
Cell Behavior

scholarly journals A minimal standardized human bone marrow microphysiological system to assess resident cell behavior during normal and pathological processes

2022 ◽  
Author(s):  
Thibault Voeltzel ◽  
Gaëlle Fossard ◽  
Michaël Degaud ◽  
Kevin Geistlich ◽  
Nicolas Gadot ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Viable Cell ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cell Behavior ◽  
Cell Harvesting ◽  
Resident Cell ◽  
Level Of Analysis

We provide an easy to access microphysiological standardized system approaching the human bone marrow complexity to a first level of analysis by in situ imaging or by viable cell harvesting of processes taking place within this ecosystem.

scholarly journals Orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression

2017 ◽  
Vol 28 (14) ◽  
pp. 1997-2009 ◽  
Author(s):  
Yejun Wang ◽  
Mallika Nagarajan ◽  
Caroline Uhler ◽  
G. V. Shivashankar
Keyword(s):  
Gene Expression ◽  
Expression Patterns ◽  
Three Dimensional ◽  
Gene Expression Patterns ◽  
Cell Behavior ◽  
Global Changes ◽  
Cell Geometry ◽  
Transcription Inhibition ◽  
Regulate Gene Expression

Extracellular matrix signals from the microenvironment regulate gene expression patterns and cell behavior. Using a combination of experiments and geometric models, we demonstrate correlations between cell geometry, three-dimensional (3D) organization of chromosome territories, and gene expression. Fluorescence in situ hybridization experiments showed that micropatterned fibroblasts cultured on anisotropic versus isotropic substrates resulted in repositioning of specific chromosomes, which contained genes that were differentially regulated by cell geometries. Experiments combined with ellipsoid packing models revealed that the mechanosensitivity of chromosomes was correlated with their orientation in the nucleus. Transcription inhibition experiments suggested that the intermingling degree was more sensitive to global changes in transcription than to chromosome radial positioning and its orientations. These results suggested that cell geometry modulated 3D chromosome arrangement, and their neighborhoods correlated with gene expression patterns in a predictable manner. This is central to understanding geometric control of genetic programs involved in cellular homeostasis and the associated diseases.

scholarly journals Pathogen Trojan Horse Delivers Bioactive Host Protein to Alter Maize Anther Cell Behavior in Situ

2018 ◽  
Vol 30 (3) ◽  
pp. 528-542 ◽  
Author(s):  
Karina van der Linde ◽  
Ljudmilla Timofejeva ◽  
Rachel L. Egger ◽  
Birger Ilau ◽  
Reza Hammond ◽  
...  
Keyword(s):  
Trojan Horse ◽  
Host Protein ◽  
Cell Behavior

scholarly journals Rapid Bioorthogonal Chemistry Enables in Situ Modulation of the Stem Cell Behavior in 3D without External Triggers

2018 ◽  
Vol 10 (31) ◽  
pp. 26016-26027 ◽  
Author(s):  
Ying Hao ◽  
Jiyeon Song ◽  
Anitha Ravikrishnan ◽  
Kevin T. Dicker ◽  
Eric W. Fowler ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Behavior ◽  
Bioorthogonal Chemistry
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