Time Evolution of Nanoparticle–Protein Corona in Human Plasma: Relevance for Targeted Drug Delivery

Langmuir ◽  
2013 ◽  
Vol 29 (21) ◽  
pp. 6485-6494 ◽  
Author(s):  
Ana Lilia Barrán-Berdón ◽  
Daniela Pozzi ◽  
Giulio Caracciolo ◽  
Anna Laura Capriotti ◽  
Giuseppe Caruso ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Human Plasma ◽  
Time Evolution ◽  
Targeted Drug Delivery ◽  
Protein Corona ◽  
Targeted Drug

scholarly journals Cloaking nanoparticles with protein corona shield for targeted drug delivery

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Jun Yong Oh ◽  
Han Sol Kim ◽  
L. Palanikumar ◽  
Eun Min Go ◽  
Batakrishna Jana ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Targeted Drug Delivery ◽  
Protein Corona ◽  
Targeted Drug

scholarly journals Understanding the Factors Influencing Chitosan-Based Nanoparticles-Protein Corona Interaction and Drug Delivery Applications

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4758
Author(s):  
Cristina Moraru ◽  
Manuela Mincea ◽  
Gheorghita Menghiu ◽  
Vasile Ostafe
Keyword(s):  
Drug Delivery ◽  
Targeted Drug Delivery ◽  
Biological Fluids ◽  
Chemical Properties ◽  
Protein Corona ◽  
Target Cells ◽  
Major Application ◽  
Targeted Drug ◽  
Drug Delivery Applications

Chitosan is a polymer that is extensively used to prepare nanoparticles (NPs) with tailored properties for applications in many fields of human activities. Among them, targeted drug delivery, especially when cancer therapy is the main interest, is a major application of chitosan-based NPs. Due to its positive charges, chitosan is used to produce the core of the NPs or to cover NPs made from other types of polymers, both strategies aiming to protect the carried drug until NPs reach the target sites and to facilitate the uptake and drug delivery into these cells. A major challenge in the design of these chitosan-based NPs is the formation of a protein corona (PC) upon contact with biological fluids. The composition of the PC can, to some extent, be modulated depending on the size, shape, electrical charge and hydrophobic/hydrophilic characteristics of the NPs. According to the composition of the biological fluids that have to be crossed during the journey of the drug-loaded NPs towards the target cells, the surface of these particles can be changed by covering their core with various types of polymers or with functionalized polymers carrying some special molecules, that will preferentially adsorb some proteins in their PC. The PC’s composition may change by continuous processes of adsorption and desorption, depending on the affinity of these proteins for the chemical structure of the surface of NPs. Beside these, in designing the targeted drug delivery NPs one can take into account their toxicity, initiation of an immune response, participation (enhancement or inhibition) in certain metabolic pathways or chemical processes like reactive oxygen species, type of endocytosis of target cells, and many others. There are cases in which these processes seem to require antagonistic properties of nanoparticles. Products that show good behavior in cell cultures may lead to poor in vivo results, when the composition of the formed PC is totally different. This paper reviews the physico-chemical properties, cellular uptake and drug delivery applications of chitosan-based nanoparticles, specifying the factors that contribute to the success of the targeted drug delivery. Furthermore, we highlight the role of the protein corona formed around the NP in its intercellular fate.

scholarly journals Novel brain-targeted nanomicelles for anti-glioma therapy mediated by the ApoE-enriched protein corona in vivo

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Zhe-Ao Zhang ◽  
Xin Xin ◽  
Chao Liu ◽  
Yan-hong Liu ◽  
Hong-Xia Duan ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Targeted Drug Delivery ◽  
Protein Corona ◽  
Lipid Binding ◽  
Binding Domain ◽  
Amyloid Β Protein ◽  
Blood Brain ◽  
Targeted Drug

Abstract Background The interactions between nanoparticles (NPs) and plasma proteins form a protein corona around NPs after entering the biological environment, which provides new biological properties to NPs and mediates their interactions with cells and biological barriers. Given the inevitable interactions, we regard nanoparticle‒protein interactions as a tool for designing protein corona-mediated drug delivery systems. Herein, we demonstrate the successful application of protein corona-mediated brain-targeted nanomicelles in the treatment of glioma, loading them with paclitaxel (PTX), and decorating them with amyloid β-protein (Aβ)-CN peptide (PTX/Aβ-CN-PMs). Aβ-CN peptide, like the Aβ1–42 peptide, specifically binds to the lipid-binding domain of apolipoprotein E (ApoE) in vivo to form the ApoE-enriched protein corona surrounding Aβ-CN-PMs (ApoE/PTX/Aβ-CN-PMs). The receptor-binding domain of the ApoE then combines with low-density lipoprotein receptor (LDLr) and LDLr-related protein 1 receptor (LRP1r) expressed in the blood–brain barrier and glioma, effectively mediating brain-targeted delivery. Methods PTX/Aβ-CN-PMs were prepared using a film hydration method with sonication, which was simple and feasible. The specific formation of the ApoE-enriched protein corona around nanoparticles was characterized by Western blotting analysis and LC–MS/MS. The in vitro physicochemical properties and in vivo anti-glioma effects of PTX/Aβ-CN-PMs were also well studied. Results The average size and zeta potential of PTX/Aβ-CN-PMs and ApoE/PTX/Aβ-CN-PMs were 103.1 nm, 172.3 nm, 7.23 mV, and 0.715 mV, respectively. PTX was efficiently loaded into PTX/Aβ-CN-PMs, and the PTX release from rhApoE/PTX/Aβ-CN-PMs exhibited a sustained-release pattern in vitro. The formation of the ApoE-enriched protein corona significantly improved the cellular uptake of Aβ-CN-PMs on C6 cells and human umbilical vein endothelial cells (HUVECs) and enhanced permeability to the blood–brain tumor barrier in vitro. Meanwhile, PTX/Aβ-CN-PMs with ApoE-enriched protein corona had a greater ability to inhibit cell proliferation and induce cell apoptosis than taxol. Importantly, PTX/Aβ-CN-PMs exhibited better anti-glioma effects and tissue distribution profile with rapid accumulation in glioma tissues in vivo and prolonged median survival of glioma-bearing mice compared to those associated with PMs without the ApoE protein corona. Conclusions The designed PTX/Aβ-CN-PMs exhibited significantly enhanced anti-glioma efficacy. Importantly, this study provided a strategy for the rational design of a protein corona-based brain-targeted drug delivery system. More crucially, we utilized the unfavorable side of the protein corona and converted it into an advantage to achieve brain-targeted drug delivery. Graphical Abstract

scholarly journals Brain-targeted drug delivery by manipulating protein corona functions

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Zui Zhang ◽  
Juan Guan ◽  
Zhuxuan Jiang ◽  
Yang Yang ◽  
Jican Liu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Targeted Drug Delivery ◽  
Protein Corona ◽  
Targeted Drug

Impact of PEGylated Nanoparticles on Tumor Targeted Drug Delivery

2018 ◽  
Vol 24 (28) ◽  
pp. 3283-3296 ◽  
Author(s):  
Fitya Syarifa Mozar ◽  
Ezharul Hoque Chowdhury
Keyword(s):  
Drug Delivery ◽  
Targeted Drug Delivery ◽  
Surface Characteristics ◽  
Protein Corona ◽  
Therapeutic Agents ◽  
Life Extension ◽  
Pegylated Nanoparticles ◽  
Dosing Frequency ◽  
Targeted Drug ◽  
Tumor Accumulation

PEG-functionalized nanoparticles as carriers of chemotherapeutics agents have been explored with notable successes in preclinical and clinical stages of cancer treatment, with some already approved by FDA, namely PEGylated liposomes and polymers. Half-life extension of therapeutic agents through PEGylation process improves their pharmacokinetic (PK) profiles, thereby reducing their dosing frequency. Protein corona composition of PEGylated nanoparticles (NPs) confers a tremendous influence on their surface characteristics which directly impact tumor accumulation and clearance properties of the drugs. By controlling the size and complexity of PEG molecules, as well as by attaching targeting moieties, the surface characteristics of NPs can be manipulated to improve their tumor uptake without sacrificing the circulation time. This review focuses on design and applications of PEGylated NPs for tumor targeted drug delivery in animal models and clinical setting.

A proteomics-based methodology to investigate the protein corona effect for targeted drug delivery

2014 ◽  
Vol 10 (11) ◽  
pp. 2815-2819 ◽  
Author(s):  
D. Pozzi ◽  
G. Caracciolo ◽  
A. L. Capriotti ◽  
C. Cavaliere ◽  
S. Piovesana ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drug Delivery ◽  
Tandem Mass Spectrometry ◽  
Targeted Drug Delivery ◽  
Protein Corona ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Targeted Drug ◽  
Nanoliquid Chromatography

Here we introduce a proteomics methodology based on nanoliquid-chromatography tandem mass spectrometry (nanoLC/MS-MS) to investigate the “protein corona effect for targeted drug delivery”.

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