Preparation and Characterization of Liposome-Encapsulated Plasmid DNA for Gene Delivery

Langmuir ◽  
2013 ◽  
Vol 29 (29) ◽  
pp. 9208-9215 ◽  
Author(s):  
Rachel M. Levine ◽  
Timothy R. Pearce ◽  
Maroof Adil ◽  
Efrosini Kokkoli
Keyword(s):  
Gene Delivery ◽  
Plasmid Dna

Preparation and Characterization of Thermosensitive Submicron Particles for Gene Delivery

2006 ◽  
Vol 6 (9) ◽  
pp. 2804-2810 ◽  
Author(s):  
Nalan Özdemir ◽  
Ali Tuncel ◽  
Myungchan Kang ◽  
Emir Baki Denkbş
Keyword(s):  
Gene Delivery ◽  
Plasmid Dna ◽  
Aqueous Media ◽  
Monomer Concentration ◽  
Mouse Fibroblast ◽  
Submicron Particles ◽  
Dna Adsorption ◽  
Different Temperatures ◽  
Total Monomer Concentration

In this study, a new thermosensitive material was proposed as a carrier for gene delivery. The thermosensitive submicron particles were synthesized by the dispersion copolymerization of N-isopropylacylamide (NIPA) with a relatively new, cationic comonomer, N-3-dimethylamino-propylmethacrylamide (DMAPM) with higher ionization ability with respect to the commonly used cationic comonomers. To achieve particle sizes smaller than 1 μm, suitable for gene delivery, the total monomer concentration in the dispersion copolymerization was kept at a sufficiently low level. The size of poly(NIPA-co-DMAPM) particles was determined as 454 nm, by AFM in dry state. The poly(NIPA-co-DMAPM) particles showed both temperature and pH sensitivity in the aqueous media. The plasmid DNA adsorption onto the thermosensitive cationic particles was investigated at different temperatures and pHs. The adsorbed amount of plasmid DNA onto the particles was significantly increased by the introduction of cationic comonomer. The equilibrium plasmid DNA adsorptions up to 13 mg/g dry particles were achieved at physiological pH. Approximately 36% w/w of adsorbed plasmid could be desorbed from the cationic nanolatex. The results of biocompatibility studies performed with mouse fibroblast cells showed the suitability of thermosensitive cationic particles for intended application.

scholarly journals Characterization of liposome-mediated gene delivery: expression, stability and pharmacokinetics of plasmid DNA

Gene Therapy ◽  
1997 ◽  
Vol 4 (3) ◽  
pp. 226-237 ◽  
Author(s):  
AR Thierry ◽  
P Rabinovich ◽  
B Peng ◽  
LC Mahan ◽  
JL Bryant ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Plasmid Dna ◽  
Expression Stability

scholarly journals Preparation and characterization of non-viral gene delivery systems with pEGFP-C1 Plasmid DNA

2018 ◽  
Vol 54 (1) ◽  
Author(s):  
Uğur Karagöz ◽  
Mustafa Kotmakçı ◽  
Hasan Akbaba ◽  
Vildan Bozok Çetintaş ◽  
Gülten Kantarcı
Keyword(s):  
Gene Delivery ◽  
Plasmid Dna ◽  
Delivery Systems ◽  
Viral Gene ◽  
Viral Gene Delivery

Altering the characterization of nanofibers by changing the electrospinning parameters and their application in tissue engineering, drug delivery, and gene delivery systems

2021 ◽  
Vol 32 (5) ◽  
pp. 1924-1950
Author(s):  
Amir Ghaderpour ◽  
Zohreh Hoseinkhani ◽  
Reza Yarani ◽  
Sina Mohammadiani ◽  
Farshid Amiri ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Gene Delivery ◽  
Delivery Systems

Plasmid DNA microgels for cancer treatment through combination of chemotherapy and gene delivery

2014 ◽  
Vol 185 ◽  
pp. S35
Author(s):  
Diana Barata Costa ◽  
Artur Monteiro Valente ◽  
João Sampaio Queiroz
Keyword(s):  
Gene Delivery ◽  
Cancer Treatment ◽  
Plasmid Dna
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