Surface Functionalization of a Polymeric Lipid Bilayer for Coupling a Model Biological Membrane with Molecules, Cells, and Microstructures

Langmuir ◽  
2013 ◽  
Vol 29 (8) ◽  
pp. 2722-2730 ◽  
Author(s):  
Kenichi Morigaki ◽  
Kazuyuki Mizutani ◽  
Makoto Saito ◽  
Takashi Okazaki ◽  
Yoshihiro Nakajima ◽  
...  
Keyword(s):  
Lipid Bilayer ◽  
Surface Functionalization ◽  
Biological Membrane

scholarly journals Surface roughness as rupture control factor of lipid vesicles

2013 ◽  
Vol 19 (S4) ◽  
pp. 107-108 ◽  
Author(s):  
A.A. Duarte ◽  
M. Raposo
Keyword(s):  
Lipid Bilayer ◽  
Lipid Bilayers ◽  
Biological Membrane ◽  
Substrate Surface ◽  
Lipid Vesicles ◽  
Planar Lipid Bilayer ◽  
Root Mean Square Roughness ◽  
Control Factor ◽  
Formation Mechanisms ◽  
Adsorbed Amount

Liposomes or lipid vesicles are self-closed structures formed by one or several concentric lipid bilayers with an aqueous phase inside, which may incorporate almost any molecule, namely proteins, hormones, enzymes, antibiotics, anticancer agents, antifungical agents, gene transfer agents, DNA, and whole viruses. Scientific evidences prove that unprotected liposomes containing drugs are easily released from the endoplasmic reticulum of the cell. To increase the vesicles lifetime and to activate a controlled drug release with an external stimulus, the vesicles immobilization on a surface and the factors which create conditions to the liposome rupture have to be analyzed. A number of studies have identified some of the critical stages of vesicle adsorption (adhesion), fusion, deformation, rupture, and spreading of the lipid bilayer. Nevertheless, the formation mechanisms of well-controlled continuous supported bilayers or adsorption of whole liposomes are still not fully understood. As yet it was demonstrated that a controlled adsorption of vesicles containing a small fraction of charged lipids occurs without rupture and their subsequent embedding in polyelectrolyte multilayer (PEM) films, meaning vesicles may be immobilized in an intact or slightly deformed state, which can act as drug reservoirs. Moreover, depending on the nature of the physicochemical conditions of the vesicle solution and the substrate surface, a flat lipid bilayer can be formed, known as supported lipid bilayers, which can incorporate membrane proteins and keep the native dynamics of the lipid bilayer mimicking a biological membrane. In this study, a layer of 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (sodium salt) (DPPG) liposomes adsorbed onto PEMs cushions based on poly(ethylenimine) (PEI), poly(sodium 4-styrenesulfonate) (PSS) and poly(allylamine hydrochloride) (PAH) polyelectrolytes was analyzed by atomic force microscopy (AFM) technique in non-contact mode and quartz crystal microbalance (QCM).Sequential heterostructures of Si/PEI(PSS/PAH)4 and Si/PAH, also designated cushions, were prepared onto silicon substrates using the layer-by-layer (LbL) technique with polyelectrolyte solutions of PEI, PSS and PAH of monomeric concentrations of 0.01M. Topographic images of 1×1μm2 area of Si/PAH/DPPG (Figure 1 a), and Si/PEI(PSS/PAH)4/DPPG (Figure 1 b) LbL films were acquired by AFM. The root mean square roughness (RMS) calculated from topographies data are listed in table I. As shown, when a DPPG layer is adsorbed onto Si/PAH the RMS keeps an approximately equal value meaning that the liposome disrupted and spread onto the surface forming a planar lipid bilayer. But when a DPPG layer is adsorbed onto Si/PEI(PSS/PAH)4 the RMS value doubled, indicating that the structural integrity of the liposomes is maintained, even though there has been any deformation during adsorption. The adsorbed amount of the two PEMs and DPPG-liposomes layers was measured using a QCM and is displayed in table I. The DPPG adsorbed amount obtained on the PAH cushion was approximately equal to a planar lipid bilayer, while the adsorption onto PEI(PSS/PAH)4 was higher than the predicted for a planar lipid bilayer. This behavior suggests that the DPPG liposomes on the second PEM remained intact during adsorption. Both confirm the AFM results. Therefore we conclude that the initial roughness of the surface is a primordial factor to determine the adsorption or not of intact vesicles.The authors acknowledge the “Fundação para a Ciência e Tecnologia” (FCT-MEC) by the post-graduate scholarship SFRH/BD/62229/2009 and the “Plurianual” funding.

The biophysical interaction of ferulic acid with liposomes as biological membrane model: The effect of the lipid bilayer composition

2020 ◽  
pp. 114689
Author(s):  
Stéphanie Andrade ◽  
Maria João Ramalho ◽  
Joana Angélica Loureiro ◽  
Maria Carmo Pereira
Keyword(s):  
Ferulic Acid ◽  
Lipid Bilayer ◽  
Biological Membrane ◽  
Membrane Model

Protein tethered lipid bilayer: An alternative mimic of the biological membrane (Mini Review)

2008 ◽  
Vol 3 (2) ◽  
pp. FA101-FA107 ◽  
Author(s):  
Renate L. C. Naumann ◽  
Wolfgang Knoll
Keyword(s):  
Lipid Bilayer ◽  
Biological Membrane

Phosphonium carbosilane dendrimers – interaction with a simple biological membrane model

2018 ◽  
Vol 20 (21) ◽  
pp. 14753-14764 ◽  
Author(s):  
Dominika Wrobel ◽  
Radka Kubikova ◽  
Monika Müllerová ◽  
Tomas Strašák ◽  
Květoslav Růžička ◽  
...  
Keyword(s):  
Lipid Bilayer ◽  
Biological Membrane ◽  
Membrane Model ◽  
Carbosilane Dendrimers

Factors such as shielding of charge on dendrimers by bulky substituents and/or hydrophobicity of substituents are important for final ability of dendrimers to interact with and to penetrate deep into the lipid bilayer.

scholarly journals The importance of intramolecular hydrogen bonds on the translocation of the small drug piracetam through a lipid bilayer

RSC Advances ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 899-908
Author(s):  
João T. S. Coimbra ◽  
Ralph Feghali ◽  
Rui P. Ribeiro ◽  
Maria J. Ramos ◽  
Pedro A. Fernandes
Keyword(s):  
Hydrogen Bonds ◽  
Lipid Bilayer ◽  
Biological Membrane ◽  
Intramolecular Hydrogen Bonds ◽  
Membrane Models ◽  
Intramolecular Hydrogen ◽  
The Impact

Using computational strategies and an analogue compound, we explore and measure the impact of intramolecular hydrogen bonds on the translocation of the small drug piracetam, through biological membrane models.

scholarly journals Partial molecular volumes of cholesterol and phosphatidylcholine in mixed bilayers

2017 ◽  
Vol 64 (2) ◽  
pp. 1-3 ◽  
Author(s):  
J. Gallová ◽  
K. Želinská ◽  
P. Balgavý
Keyword(s):  
Phase Transition ◽  
Simple Model ◽  
Lipid Bilayer ◽  
Specific Volume ◽  
Biological Membrane ◽  
Phase Behaviour ◽  
Molecular Volume ◽  
Ideal Mixing ◽  
Multilamellar Liposomes ◽  
Increasing Temperature

Abstract Dispersion of multilamellar liposomes of dimyristoylphosphatidylcholine (DMPC) and cholesterol (CHOL) were studied by vibrational densitometer for the CHOL mole fractions X = 0−0.54 in the temperature range 18−50 °C, both below and above the main phase transition. DMPC-CHOL bilayers served as a simple model for lipidic part of biological membrane. Volumetric parameters are essential not only to evaluate the data obtained by scattering and diffraction methods on model membranes but can provide valuable information about molecular packing in bilayers and the phase behaviour of lipid-CHOL mixtures. In this paper, preliminary results regarding the changes in the specific volume of lipid bilayer with increasing temperature and CHOL content are presented. Different values of apparent molecular volume of CHOL for different CHOL mole fraction pointed out the non-ideal mixing of DMPC and CHOL.

scholarly journals The Protein-Tethered Lipid Bilayer: A Novel Mimic of the Biological Membrane

2004 ◽  
Vol 87 (5) ◽  
pp. 3213-3220 ◽  
Author(s):  
Frank Giess ◽  
Marcel G. Friedrich ◽  
Joachim Heberle ◽  
Renate L. Naumann ◽  
Wolfgang Knoll
Keyword(s):  
Lipid Bilayer ◽  
Biological Membrane

scholarly journals Delivery of Alpha-Mangostin Using Cyclodextrins through a Biological Membrane: Molecular Dynamics Simulation

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2532
Author(s):  
Wiparat Hotarat ◽  
Bodee Nutho ◽  
Peter Wolschann ◽  
Thanyada Rungrotmongkol ◽  
Supot Hannongbua
Keyword(s):  
Molecular Dynamics ◽  
Molecular Dynamics Simulation ◽  
Complex Formation ◽  
Inclusion Complex ◽  
Lipid Bilayer ◽  
Water Solubility ◽  
Biological Membrane ◽  
Membrane Surface ◽  
Dynamics Simulation ◽  
Electrostatic Energy

α-Mangostin (MGS) exhibits various pharmacological activities, including antioxidant, anticancer, antibacterial, and anti-inflammatory properties. However, its low water solubility is the major obstacle for its use in pharmaceutical applications. To increase the water solubility of MGS, complex formation with beta-cyclodextrins (βCDs), particularly with the native βCD and/or its derivative 2,6-dimethyl-β-CD (DMβCD) is a promising technique. Although there have been several reports on the adsorption of βCDs on the lipid bilayer, the release of the MGS/βCDs inclusion complex through the biological membrane remains unclear. In this present study, the release the MGS from the two different βCDs (βCD and DMβCD) across the lipid bilayer was investigated. Firstly, the adsorption of the free MGS, free βCDs, and inclusion complex formation was studied by conventional molecular dynamics simulation. The MGS in complex with those two βCDs was able to spontaneously release free MGS into the inner membrane. However, both MGS and DMβCD molecules potentially permeated into the deeper region of the interior membrane, whereas βCD only adsorbed at the outer membrane surface. The interaction between secondary rim of βCD and the 1-palmitoeyl-2-oleoyl-glycero-3-phosphocholine (POPC) phosphate groups showed the highest number of hydrogen bonds (up to 14) corresponding to the favorable location of βCD on the POPC membrane. Additionally, the findings suggested that electrostatic energy was the main driving force for βCD adsorption on the POPC membrane, while van der Waals interactions played a predominant role in DMβCD adsorption. The release profile of MGS from the βCDs pocket across the lipid bilayer exhibited two energy minima along the reaction coordinate associated with the permeation of the MGS molecule into the deeper region of the POPC membrane.

Nano-pore Lipid Bilayer Formed by Self-spreading Method

2019 ◽  
Vol 139 (10) ◽  
pp. 1146-1152
Author(s):  
Zugui Peng ◽  
Kenta Shimba ◽  
Yoshitaka Miyamoto ◽  
Tohru Yagi
Keyword(s):  
Lipid Bilayer
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