scholarly journals Disruption of Escherichia coli Amyloid-Integrated Biofilm Formation at the Air–Liquid Interface by a Polysorbate Surfactant

Langmuir ◽  
2013 ◽  
Vol 29 (3) ◽  
pp. 920-926 ◽  
Author(s):  
Cynthia Wu ◽  
Ji Youn Lim ◽  
Gerald G. Fuller ◽  
Lynette Cegelski
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Liquid Interface ◽  
Air Liquid Interface

scholarly journals Biofilm Formation by and Multicellular Behavior of Escherichia coli O55:H7, an Atypical Enteropathogenic Strain

2010 ◽  
Vol 76 (5) ◽  
pp. 1545-1554 ◽  
Author(s):  
Michal Weiss-Muszkat ◽  
Dana Shakh ◽  
Yizhou Zhou ◽  
Riky Pinto ◽  
Eddy Belausov ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Genetic Relatedness ◽  
Liquid Interface ◽  
E Coli ◽  
Wild Type Phenotype ◽  
Enteropathogenic Strain ◽  
Biofilm Development ◽  
Air Liquid Interface ◽  
High Degree

ABSTRACT Enteropathogenic Escherichia coli (EPEC) is an important causal agent of diarrheal illness throughout the world. Nevertheless, researchers have only recently begun to explore its capacity to form biofilms. Strain O55:H7 (DMS9) is a clinical isolate belonging to the atypical EPEC (aEPEC) group, which displays a high degree of genetic relatedness to enterohemorrhagic E. coli. Strain DMS9 formed a robust biofilm on an abiotic surface at 26�C, but not at 37�C. It also formed a dense pellicle at the air-liquid interface and developed a red, rough, and dry (RDAR) morphotype on Congo red agar. Unlike a previously described E. coli O157:H7 strain, the aEPEC strain seems to express cellulose. Transposon mutagenesis was used to identify biofilm-deficient mutants. One of the mutants was inactivated in the csgFG genes, required for assembly and secretion of curli fimbriae, while a second mutant had a mutation in crl, a thermosensitive global regulator that modulates σS activity and downstream expression of curli and cellulose. The two mutants were deficient in their biofilm formation capabilities and did not form a pellicle at the air-liquid interface. Unlike in Salmonella, the csgFG mutant in aEPEC completely lost the RDAR phenotype, while the crl mutant displayed a unique RDAR “pizza”-like morphotype. Genetic complementation of the two mutants resulted in restoration of the wild-type phenotype. This report is the first to describe and analyze a multicellular behavior in aEPEC and support a major role for curli and the crl regulator in biofilm development at low temperatures corresponding to the nonmammalian host environment.

scholarly journals Biofilm formation by South African non-O157 Shiga toxigenic Escherichia coli on stainless steel coupons

2020 ◽  
Vol 66 (4) ◽  
pp. 328-336 ◽  
Author(s):  
Emmanuel W. Bumunang ◽  
Collins N. Ateba ◽  
Kim Stanford ◽  
Tim A. McAllister ◽  
Yan D. Niu
Keyword(s):  
Escherichia Coli ◽  
Stainless Steel ◽  
South African ◽  
Food Processing ◽  
Biofilm Formation ◽  
Viable Cell ◽  
Liquid Interface ◽  
Cell Counts ◽  
Potential Source ◽  
Air Liquid Interface

This study examined the biofilm-forming ability of six non-O157 Shiga-toxin-producing Escherichia coli (STEC) strains: O116:H21, wzx-Onovel5:H19, O129:H21, O129:H23, O26:H11, and O154:H10 on stainless steel coupons after 24, 48, and 72 h of incubation at 22 °C and after 168 h at 10 °C. The results of crystal violet staining revealed that strains O129:H23 and O154:H10 were able to form biofilms on both the submerged surface and the air–liquid interface of coupons, whereas strains O116:H21, wzx-Onovel5:H19, O129:H21, and O26:H11 formed biofilm only at the air–liquid interface. Viable cell counts and scanning electron microscopy showed that biofilm formation increased (p < 0.05) over time. The biofilm-forming ability of non-O157 STEC was strongest (p < 0.05) at 22 °C after 48 h of incubation. The strongest biofilm former regardless of temperature was O129:H23. Generally, at 10 °C, weak to no biofilm was observed for isolates O154:H10, O116:H21, wzx-Onovel5:H19, O26:H11, and O129:H21 after 168 h. This study found that temperature affected the biofilm-forming ability of non-O157 STEC strains. Overall, our data indicate a high potential for biofilm formation by the isolates at 22 °C, suggesting that non-O157 STEC strains could colonize stainless steel within food-processing facilities. This could serve as a potential source of adulteration and promote the dissemination of these potential pathogens in food.

Characterization of biofilm formation by Salmonella enterica at the air-liquid interface in aquatic environments

2018 ◽  
Vol 190 (4) ◽  
Author(s):  
José Andrés Medrano-Félix ◽  
Cristóbal Chaidez ◽  
Kristina D. Mena ◽  
María del Socorro Soto-Galindo ◽  
Nohelia Castro-del Campo
Keyword(s):  
Salmonella Enterica ◽  
Biofilm Formation ◽  
Liquid Interface ◽  
Aquatic Environments ◽  
Air Liquid Interface

scholarly journals Increased Transfer of a Multidrug Resistance Plasmid in Escherichia coli Biofilms at the Air-Liquid Interface

2011 ◽  
Vol 77 (15) ◽  
pp. 5079-5088 ◽  
Author(s):  
Jaroslaw E. Król ◽  
Hung Duc Nguyen ◽  
Linda M. Rogers ◽  
Haluk Beyenal ◽  
Stephen M. Krone ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Gene Transfer ◽  
Flow Cell ◽  
Plasmid Transfer ◽  
Liquid Interface ◽  
Transfer Efficiency ◽  
Content Type ◽  
Resistance Plasmid ◽  
Air Liquid Interface

ABSTRACTAlthough biofilms represent a common bacterial lifestyle in clinically and environmentally important habitats, there is scant information on the extent of gene transfer in these spatially structured populations. The objective of this study was to gain insight into factors that affect transfer of the promiscuous multidrug resistance plasmid pB10 inEscherichia colibiofilms. Biofilms were grown in different experimental settings, and plasmid transfer was monitored using laser scanning confocal microscopy and plate counting. In closed flow cells, plasmid transfer in surface-attached submerged biofilms was negligible. In contrast, a high plasmid transfer efficiency was observed in a biofilm floating at the air-liquid interface in an open flow cell with low flow rates. A vertical flow cell and a batch culture biofilm reactor were then used to detect plasmid transfer at different depths away from the air-liquid interface. Extensive plasmid transfer occurred only in a narrow zone near that interface. The much lower transfer frequencies in the lower zones coincided with rapidly decreasing oxygen concentrations. However, when anE. colicsrAmutant was used as the recipient, a thick biofilm was obtained at all depths, and plasmid transfer occurred at similar frequencies throughout. These results and data from separate aerobic and anaerobic matings suggest that oxygen can affect IncP-1 plasmid transfer efficiency, not only directly but also indirectly, through influencing population densities and therefore colocalization of donors and recipients. In conclusion, the air-liquid interface can be a hot spot for plasmid-mediated gene transfer due to high densities of juxtaposed donor and recipient cells.

scholarly journals Counterclockwise Circular Motion of Bacteria Swimming at the Air-Liquid Interface

2010 ◽  
Vol 192 (23) ◽  
pp. 6307-6308 ◽  
Author(s):  
Laurence Lemelle ◽  
Jean-François Palierne ◽  
Elodie Chatre ◽  
Christophe Place
Keyword(s):  
Escherichia Coli ◽  
Liquid Interface ◽  
Circular Motion ◽  
Solid Surfaces ◽  
Bacterial Swimming ◽  
Air Liquid Interface ◽  
Key Parameter

ABSTRACT Flagellar propulsion of swimming Escherichia coli produces circling clockwise motions near planar solid surfaces. Counterclockwise motion was first reported near air-TN medium interfaces, showing that slip at the interface is a key parameter of bacterial swimming.

scholarly journals Extending an Eco-Evolutionary Understanding of Biofilm-Formation at the Air-Liquid Interface to Community Biofilms

2020 ◽  
Author(s):  
Robyn Jerdan ◽  
Olga Iungin ◽  
Olena V. Moshynets ◽  
Geert Potters ◽  
Andrew J. Spiers
Keyword(s):  
Biofilm Formation ◽  
Liquid Interface ◽  
Air Liquid Interface

Mechanical and microstructural insights of Vibrio cholerae and Escherichia coli dual-species biofilm at the air-liquid interface

2020 ◽  
Vol 188 ◽  
pp. 110786 ◽  
Author(s):  
Clémence Abriat ◽  
Kyle Enriquez ◽  
Nick Virgilio ◽  
Lynette Cegelski ◽  
Gerald G. Fuller ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Vibrio Cholerae ◽  
Liquid Interface ◽  
Air Liquid Interface

scholarly journals Biofilm Formation by Shiga Toxin-Producing Escherichia coli on Stainless Steel Coupons as Affected by Temperature and Incubation Time

2019 ◽  
Vol 7 (4) ◽  
pp. 95 ◽  
Author(s):  
Zhi Ma ◽  
Emmanuel W. Bumunang ◽  
Kim Stanford ◽  
Xiaomei Bie ◽  
Yan D. Niu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Stainless Steel ◽  
Shiga Toxin ◽  
Incubation Time ◽  
Food Processing ◽  
Biofilm Formation ◽  
Crystal Violet Staining ◽  
Plate Counts ◽  
Air Liquid Interface ◽  
Over Time

Forming biofilm is a strategy utilized by Shiga toxin-producing Escherichia coli (STEC) to survive and persist in food processing environments. We investigated the biofilm-forming potential of STEC strains from 10 clinically important serogroups on stainless steel at 22 °C or 13 °C after 24, 48, and 72 h of incubation. Results from crystal violet staining, plate counts, and scanning electron microscopy (SEM) identified a single isolate from each of the O113, O145, O91, O157, and O121 serogroups that was capable of forming strong or moderate biofilms on stainless steel at 22 °C. However, the biofilm-forming strength of these five strains was reduced when incubation time progressed. Moreover, we found that these strains formed a dense pellicle at the air-liquid interface on stainless steel, which suggests that oxygen was conducive to biofilm formation. At 13 °C, biofilm formation by these strains decreased (P < 0.05), but gradually increased over time. Overall, STEC biofilm formation was most prominent at 22 °C up to 24 h. The findings in this study identify the environmental conditions that may promote STEC biofilm formation in food processing facilities and suggest that the ability of specific strains to form biofilms contributes to their persistence within these environments.

scholarly journals Comparison of the Genetic Features Involved in Bacillus subtilis Biofilm Formation Using Multi-Culturing Approaches

2021 ◽  
Vol 9 (3) ◽  
pp. 633
Author(s):  
Yasmine Dergham ◽  
Pilar Sanchez-Vizuete ◽  
Dominique Le Coq ◽  
Julien Deschamps ◽  
Arnaud Bridier ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Biofilm Formation ◽  
Negative Impact ◽  
Liquid Interface ◽  
Subtilis Strain ◽  
Pellicle Formation ◽  
Biofilm Model ◽  
Genetic Features ◽  
Semi Solid ◽  
Air Liquid Interface

Surface-associated multicellular assemblage is an important bacterial trait to withstand harsh environmental conditions. Bacillus subtilis is one of the most studied Gram-positive bacteria, serving as a model for the study of genetic pathways involved in the different steps of 3D biofilm formation. B. subtilis biofilm studies have mainly focused on pellicle formation at the air-liquid interface or complex macrocolonies formed on nutritive agar. However, only few studies focus on the genetic features of B. subtilis submerged biofilm formation and their link with other multicellular models at the air interface. NDmed, an undomesticated B. subtilis strain isolated from a hospital, has demonstrated the ability to produce highly structured immersed biofilms when compared to strains classically used for studying B. subtilis biofilms. In this contribution, we have conducted a multi-culturing comparison (between macrocolony, swarming, pellicle, and submerged biofilm) of B. subtilis multicellular communities using the NDmed strain and mutated derivatives for genes shown to be required for motility and biofilm formation in pellicle and macrocolony models. For the 15 mutated NDmed strains studied, all showed an altered phenotype for at least one of the different culture laboratory assays. Mutation of genes involved in matrix production (i.e., tasA, epsA-O, cap, ypqP) caused a negative impact on all biofilm phenotypes but favored swarming motility on semi-solid surfaces. Mutation of bslA, a gene coding for an amphiphilic protein, affected the stability of the pellicle at the air-liquid interface with no impact on the submerged biofilm model. Moreover, mutation of lytF, an autolysin gene required for cell separation, had a greater effect on the submerged biofilm model than that formed at aerial level, opposite to the observation for lytABC mutant. In addition, B. subtilis NDmed with sinR mutation formed wrinkled macrocolony, less than that formed by the wild type, but was unable to form neither thick pellicle nor structured submerged biofilm. The results are discussed in terms of the relevancy to determine whether genes involved in colony and pellicle formation also govern submerged biofilm formation, by regarding the specificities in each model.

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