Glycopolymer Brushes for the Affinity Adsorption of RCA120: Effects of Thickness, Grafting Density, and Epitope Density

Langmuir ◽  
2012 ◽  
Vol 28 (38) ◽  
pp. 13616-13623 ◽  
Author(s):  
Xiang-Lin Meng ◽  
Yan Fang ◽  
Ling-Shu Wan ◽  
Xiao-Jun Huang ◽  
Zhi-Kang Xu
Keyword(s):  
Affinity Adsorption ◽  
Grafting Density ◽  
Epitope Density

Characterization of an Abnormal Antithrombin (Milano 2) with Defective Thrombin Binding

1986 ◽  
Vol 56 (03) ◽  
pp. 349-352 ◽  
Author(s):  
A Tripodi ◽  
A Krachmalnicoff ◽  
P M Mannucci
Keyword(s):  
Venous Thromboembolism ◽  
Active Site ◽  
Inhibitory Activity ◽  
Antithrombin Iii ◽  
Factor Xa ◽  
Molecular Defect ◽  
Affinity Adsorption ◽  
Italian Family ◽  
Crossed Immunoelectrophoresis

SummaryFour members of an Italian family (two with histories of venous thromboembolism) had a qualitative defect of antithrombin III reflected by normal antigen concentrations and halfnormal antithrombin activity with or without heparin. Anti-factor Xa activities were consistently borderline low (about 70% of normal). For the propositus’ plasma and serum the patterns of antithrombin III in crossed-immunoelectrophoresis with or without heparin were indistinguishable from those of normal plasma or serum. A normal affinity of antithrombin III for heparin was documented by heparin-sepharose chromatography. Affinity adsorption of the propositus’ plasma to human α-thrombin immobilized on sepharose beads revealed defective binding of the anti thrombin III to thrombin-sepharose. Hence the molecular defect of this variant appears to be at the active site responsible for binding and neutralizing thrombin, thus accounting for the low thrombin inhibitory activity.

scholarly journals Improved Tumor Targeting and Decreased Normal Tissue Accumulation through Extracorporeal Affinity Adsorption in a Two-Step Pretargeting Strategy

2007 ◽  
Vol 13 (18) ◽  
pp. 5572s-5576s ◽  
Author(s):  
Linda Mårtensson ◽  
Rune Nilsson ◽  
Tomas Ohlsson ◽  
Hans-Olov Sjögren ◽  
Sven-Erik Strand ◽  
...  
Keyword(s):  
Normal Tissue ◽  
Tumor Targeting ◽  
Affinity Adsorption ◽  
Tissue Accumulation

scholarly journals Bottlebrush polymers in the melt and polyelectrolytes in solution share common structural features

2020 ◽  
Vol 117 (10) ◽  
pp. 5168-5175 ◽  
Author(s):  
Joel M. Sarapas ◽  
Tyler B. Martin ◽  
Alexandros Chremos ◽  
Jack F. Douglas ◽  
Kathryn L. Beers
Keyword(s):  
Neutron Scattering ◽  
Correlation Length ◽  
Salt Concentration ◽  
Polymer Concentration ◽  
Structural Features ◽  
Peak Position ◽  
Correlation Peak ◽  
Grafting Density ◽  
Scattering Measurements ◽  
Polyelectrolyte Solutions

Uncharged bottlebrush polymer melts and highly charged polyelectrolytes in solution exhibit correlation peaks in scattering measurements and simulations. Given the striking superficial similarities of these scattering features, there may be a deeper structural interrelationship in these chemically different classes of materials. Correspondingly, we constructed a library of isotopically labeled bottlebrush molecules and measured the bottlebrush correlation peak position q*=2π/ξ by neutron scattering and in simulations. We find that the correlation length scales with the backbone concentration, ξ∼cBB−0.47, in striking accord with the scaling of ξ with polymer concentration cP in semidilute polyelectrolyte solutions (ξ∼cP−1/2). The bottlebrush correlation peak broadens with decreasing grafting density, similar to increasing salt concentration in polyelectrolyte solutions. ξ also scales with sidechain length to a power in the range of 0.35–0.44, suggesting that the sidechains are relatively collapsed in comparison to the bristlelike configurations often imagined for bottlebrush polymers.

scholarly journals Effects of Amino Acid Side-Chain Length and Chemical Structure on Anionic Polyglutamic and Polyaspartic Acid Cellulose-Based Polyelectrolyte Brushes

Polymers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1789
Author(s):  
Dmitry Tolmachev ◽  
George Mamistvalov ◽  
Natalia Lukasheva ◽  
Sergey Larin ◽  
Mikko Karttunen
Keyword(s):  
Glutamic Acid ◽  
Chain Length ◽  
Chemical Structure ◽  
Side Chain ◽  
Side Chains ◽  
Side Chain Length ◽  
Polyelectrolyte Brushes ◽  
Grafting Density ◽  
The Difference ◽  
Cellulose Surface

We used atomistic molecular dynamics (MD) simulations to study polyelectrolyte brushes based on anionic α,L-glutamic acid and α,L-aspartic acid grafted on cellulose in the presence of divalent CaCl2 salt at different concentrations. The motivation is to search for ways to control properties such as sorption capacity and the structural response of the brush to multivalent salts. For this detailed understanding of the role of side-chain length, the chemical structure and their interplay are required. It was found that in the case of glutamic acid oligomers, the longer side chains facilitate attractive interactions with the cellulose surface, which forces the grafted chains to lie down on the surface. The additional methylene group in the side chain enables side-chain rotation, enhancing this effect. On the other hand, the shorter and more restricted side chains of aspartic acid oligomers prevent attractive interactions to a large degree and push the grafted chains away from the surface. The difference in side-chain length also leads to differences in other properties of the brush in divalent salt solutions. At a low grafting density, the longer side chains of glutamic acid allow the adsorbed cations to be spatially distributed inside the brush resulting in a charge inversion. With an increase in grafting density, the difference in the total charge of the aspartic and glutamine brushes disappears, but new structural features appear. The longer sides allow for ion bridging between the grafted chains and the cellulose surface without a significant change in main-chain conformation. This leads to the brush structure being less sensitive to changes in salt concentration.

Poly(ethylene oxide) Grafted to Silicon Surfaces: Grafting Density and Protein Adsorption

1998 ◽  
Vol 31 (15) ◽  
pp. 5059-5070 ◽  
Author(s):  
Susan J. Sofia ◽  
V. Premnath ◽  
Edward W. Merrill
Keyword(s):  
Ethylene Oxide ◽  
Protein Adsorption ◽  
Silicon Surfaces ◽  
Grafting Density ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene
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