scholarly journals Mapping Single-Cell–Substrate Interactions by Surface Plasmon Resonance Microscopy

Langmuir ◽  
2012 ◽  
Vol 28 (37) ◽  
pp. 13373-13379 ◽  
Author(s):  
Wei Wang ◽  
Shaopeng Wang ◽  
Qiang Liu ◽  
Jie Wu ◽  
Nongjian Tao
Keyword(s):  
Surface Plasmon Resonance ◽  
Single Cell ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Substrate Interactions ◽  
Cell Substrate ◽  
Surface Plasmon Resonance Microscopy

scholarly journals Correlating surface plasmon resonance microscopy of living and fixated cells with electron microscopy allows for investigation of potential preparation artifacts

2019 ◽  
Author(s):  
Eva Kreysing ◽  
Silke Seyock ◽  
Hossein Hassani ◽  
Elke Brauweiler-Reuters ◽  
Elmar Neumann ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Surface Plasmon Resonance ◽  
Sample Preparation ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Chemical Fixation ◽  
Substrate Interface ◽  
Cell Substrate ◽  
Surface Plasmon Resonance Microscopy ◽  
The Impact

AbstractThe investigation of the cell-substrate interface is of great importance for a broad spectrum of areas such as biomedical engineering, brain-chip interfacing and fundamental research. Due to its unique resolution and the prevalence of instruments, electron microscopy (EM) is used as one of the standard techniques for the analysis of the cell-substrate interface. However, possible artifacts that might be introduced by the required sample preparation have been the subject of speculation for decades. Due to recent advances in Surface plasmon resonance microscopy (SPRM), the technique now offers a label-free alternative for the interface characterization with nanometer resolution in axial direction. In contrast to EM, SPRM studies do not require fixation and can therefore be performed on living cells. Here, we present a workflow that allows us to quantify the impact of chemical fixation on the cell-substrate interface. These measurements confirmed that chemical fixation preserved the average cell-substrate distances in the majority of studied cells. Furthermore, we were able to correlate the SPRM measurements with EM images of the cell-substrate interface of the exact same cells allowing us to identify regions with good agreement between the two methods and reveal artifacts introduced during further sample preparation.

scholarly journals Imaging of Cell/Substrate Contacts of Living Cells with Surface Plasmon Resonance Microscopy

1999 ◽  
Vol 76 (1) ◽  
pp. 509-516 ◽  
Author(s):  
K.-F. Giebel ◽  
C. Bechinger ◽  
S. Herminghaus ◽  
M. Riedel ◽  
P. Leiderer ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Living Cells ◽  
Cell Substrate ◽  
Surface Plasmon Resonance Microscopy
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