Tethered Bilayer Lipid Membranes on Mixed Self-Assembled Monolayers of a Novel Anchoring Thiol: Impact of the Anchoring Thiol Density on Bilayer Formation

Langmuir ◽  
2011 ◽  
Vol 27 (23) ◽  
pp. 14317-14328 ◽  
Author(s):  
Hajra Basit ◽  
Angéline Van der Heyden ◽  
Chantal Gondran ◽  
Bernard Nysten ◽  
Pascal Dumy ◽  
...  
Keyword(s):  
Lipid Membranes ◽  
Self Assembled Monolayers ◽  
Bilayer Lipid Membranes ◽  
Bilayer Lipid ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Bilayer Formation

scholarly journals The Impact of an Anchoring Layer on the Formation of Tethered Bilayer Lipid Membranes on Silver Substrates

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6878
Author(s):  
Indrė Aleknavičienė ◽  
Martynas Talaikis ◽  
Rima Budvytyte ◽  
Gintaras Valincius
Keyword(s):  
Lipid Membranes ◽  
Electrochemical Impedance ◽  
Phospholipid Bilayer ◽  
Self Assembled Monolayers ◽  
Bilayer Lipid Membranes ◽  
Vertical Orientation ◽  
Bilayer Lipid ◽  
Assembled Monolayers ◽  
Hydrophobic Lipid ◽  
The Impact

Tethered bilayer lipid membranes (tBLMs) have been known as stable and versatile experimental platforms for protein–membrane interaction studies. In this work, the assembly of functional tBLMs on silver substrates and the effect of the molecular chain-length of backfiller molecules on their properties were investigated. The following backfillers 3-mercapto-1-propanol (3M1P), 4-mercapto-1-butanol (4M1B), 6-mercapto-1-hexanol (6M1H), and 9-mercapto-1-nonanol (9M1N) mixed with the molecular anchor WC14 (20-tetradecyloxy-3,6,9,12,15,18,22 heptaoxahexatricontane-1-thiol) were used to form self-assembled monolayers (SAMs) on silver, which influenced a fusion of multilamellar vesicles and the formation of tBLMs. Spectroscopic analysis by SERS and RAIRS has shown that by using different-length backfiller molecules, it is possible to control WC14 anchor molecules orientation on the surface. An introduction of increasingly longer surface backfillers in the mixed SAM may be related to the increasing SAMs molecular order and more vertical orientation of WC14 at both the hydrophilic ethylenoxide segment and the hydrophobic lipid bilayer anchoring alkane chains. Since no clustering of WC14 alkane chains, which is deleterious for tBLM integrity, was observed on dry samples, the suitability of mixed-component SAMs for subsequent tBLM formation was further interrogated by electrochemical impedance spectroscopy (EIS). EIS showed the arrangement of well-insulating tBLMs if 3M1P was used as a backfiller. An increase in the length of the backfiller led to increased defectiveness of tBLMs. Despite variable defectiveness, all tBLMs responded to the pore-forming cholesterol-dependent cytolysin, vaginolysin in a manner consistent with the functional reconstitution of the toxin into phospholipid bilayer. This experiment demonstrates the biological relevance of tBLMs assembled on silver surfaces and indicates their utility as biosensing elements for the detection of pore-forming toxins in liquid samples.

Supported Bilayer Lipid Membrane Arrays on Photopatterned Self-Assembled Monolayers

2007 ◽  
Vol 13 (28) ◽  
pp. 7957-7964 ◽  
Author(s):  
Xiaojun Han ◽  
Singh N. D. Pradeep ◽  
Kevin Critchley ◽  
Khizar Sheikh ◽  
Richard J. Bushby ◽  
...  
Keyword(s):  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Self Assembled Monolayers ◽  
Bilayer Lipid ◽  
Supported Bilayer ◽  
Assembled Monolayers ◽  
Self Assembled

DNA Biosensor Based on Self-Assembled Bilayer Lipid Membranes for the Detection of Hydrazines

1998 ◽  
Vol 10 (10) ◽  
pp. 691-694 ◽  
Author(s):  
Christina G. Siontorou ◽  
Dimitrios P. Nikolelis ◽  
Bogdan Tarus ◽  
Julia Dumbrava ◽  
Ulrich J. Krull
Keyword(s):  
Lipid Membranes ◽  
Dna Biosensor ◽  
Bilayer Lipid Membranes ◽  
Bilayer Lipid ◽  
Self Assembled

Photoelectric conversion properties of bilayer lipid membranes self-assembled on an ITO substrate

2001 ◽  
Vol 496 (1-2) ◽  
pp. 158-161 ◽  
Author(s):  
Hong Gao ◽  
Guoan Luo ◽  
Jun Feng ◽  
Angelica L. Ottova ◽  
H.Ti Tien
Keyword(s):  
Lipid Membranes ◽  
Bilayer Lipid Membranes ◽  
Photoelectric Conversion ◽  
Bilayer Lipid ◽  
Self Assembled

Lipid Vesicle Fusion on μCP Patterned Self-Assembled Monolayers:  Effect of Pattern Geometry on Bilayer Formation

Langmuir ◽  
2002 ◽  
Vol 18 (8) ◽  
pp. 3176-3180 ◽  
Author(s):  
A. Toby A. Jenkins ◽  
Richard J. Bushby ◽  
Stephen D. Evans ◽  
Wolfgang Knoll ◽  
Andreas Offenhäusser ◽  
...  
Keyword(s):  
Vesicle Fusion ◽  
Self Assembled Monolayers ◽  
Lipid Vesicle ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Bilayer Formation

Tethered bilayer lipid membranes self-assembled on mercury electrodes

2004 ◽  
Vol 63 (1-2) ◽  
pp. 161-167 ◽  
Author(s):  
M.R. Moncelli ◽  
L. Becucci ◽  
S.M. Schiller
Keyword(s):  
Lipid Membranes ◽  
Bilayer Lipid Membranes ◽  
Bilayer Lipid ◽  
Self Assembled ◽  
Mercury Electrodes
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