Robust Method for High-Throughput Surface Patterning of Deformable Substrates

Langmuir ◽  
2011 ◽  
Vol 27 (12) ◽  
pp. 7349-7352 ◽  
Author(s):  
Ammar Azioune ◽  
Nicolas Carpi ◽  
Jenny Fink ◽  
Mohamed M. Chehimi ◽  
Damien Cuvelier ◽  
...  
Keyword(s):  
High Throughput ◽  
Surface Patterning ◽  
Robust Method

A simple and robust method for broad range screening of hair samples for drugs of abuse using a high-throughput UHPLC-Ion Trap MS instrument

2020 ◽  
Vol 1152 ◽  
pp. 122263
Author(s):  
Giacomo Musile ◽  
Mara Mazzola ◽  
Ksenia Shestakova ◽  
Sergey Savchuk ◽  
Svetlana Appolonova ◽  
...  
Keyword(s):  
High Throughput ◽  
Drugs Of Abuse ◽  
Ion Trap ◽  
Robust Method ◽  
Hair Samples

scholarly journals High Throughput pMHC-I Tetramer Library Production Using Chaperone Mediated Peptide Exchange

2019 ◽  
Author(s):  
Sarah A. Overall ◽  
Jugmohit S. Toor ◽  
Stephanie Hao ◽  
Mark Yarmarkovich ◽  
Son Nguyen ◽  
...  
Keyword(s):  
T Cell ◽  
High Throughput ◽  
Molecular Chaperone ◽  
Robust Method ◽  
Single Experiment ◽  
Mhc I ◽  
Combined Analysis ◽  
Transcription Profiles ◽  
Peptide Exchange ◽  
High Throughput Manner

ABSTRACTPeptide exchange technologies are essential for the generation of pMHC-multimer libraries, used to probe highly diverse, polyclonal TCR repertoires. Using the molecular chaperone TAPBPR, we present a robust method for the capture of stable, empty MHC-I molecules which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner. Combined with tetramer barcoding using multi-modal cellular indexing technology (ECCITE-seq), our approach allows a combined analysis of TCR repertoires and other T-cell transcription profiles together with their cognate pMHC-I specificities in a single experiment.

scholarly journals Combining a Simple Method for DNA/RNA/Protein Co-Purification and Arabidopsis Protoplast Assay to Facilitate Viroid Research

Viruses ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 324 ◽  
Author(s):  
Jian Jiang ◽  
Junfei Ma ◽  
Bin Liu ◽  
Ying Wang
Keyword(s):  
Molecular Biology ◽  
Structure Function ◽  
High Throughput ◽  
Noncoding Rnas ◽  
Protein Precipitation ◽  
New Method ◽  
Robust Method ◽  
Simple Method ◽  
Functional Studies ◽  
Arabidopsis Protoplast

Plant–viroid interactions represent a valuable model for delineating structure–function relationships of noncoding RNAs. For various functional studies, it is desirable to minimize sample variations by using DNA, RNA, and proteins co-purified from the same samples. Currently, most of the co-purification protocols rely on TRI Reagent (Trizol as a common representative) and require protein precipitation and dissolving steps, which render difficulties in experimental handling and high-throughput analyses. Here, we established a simple and robust method to minimize the precipitation steps and yield ready-to-use RNA and protein in solutions. This method can be applied to samples in small quantities, such as protoplasts. Given the ease and the robustness of this new method, it will have broad applications in virology and other disciplines in molecular biology.

scholarly journals Nitrocellulose redox permanganometry: a simple method for reductive capacity assessment

2020 ◽  
Author(s):  
J Homolak ◽  
I Kodvanj ◽  
A Babic Perhoc ◽  
D Virag ◽  
A Knezovic ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
Detailed Analysis ◽  
Manganese Dioxide ◽  
High Throughput ◽  
Biological Samples ◽  
Capacity Assessment ◽  
Robust Method ◽  
Nitrocellulose Membrane ◽  
High Throughput Analysis ◽  
Simple Method

AbstractWe propose a rapid, simple and robust method for measurement of reductive capacity of liquid and solid biological samples based on potassium permanganate reduction followed by trapping of manganese dioxide precipitate on a nitrocellulose membrane. Moreover, we discuss how nitrocellulose redox permanganometry (NRP) can be used for high-throughput analysis of biological samples and present HistoNRP, its modification used for detailed analysis of reductive capacity spatial distribution in tissue with preserved anatomical relations.

scholarly journals Droplet Microarrays: From Surface Patterning to High-Throughput Applications

2018 ◽  
Vol 30 (20) ◽  
pp. 1706111 ◽  
Author(s):  
Wenqian Feng ◽  
Erica Ueda ◽  
Pavel A. Levkin
Keyword(s):  
High Throughput ◽  
Surface Patterning

Multilaboratory Validation of a Luminex Microbead-Based Suspension Array for the Identification of the 11 Most Clinically Relevant Shiga Toxin–Producing Escherichia coli O Serogroups†

2013 ◽  
Vol 76 (5) ◽  
pp. 867-870 ◽  
Author(s):  
ANDREW LIN ◽  
JULIE A. KASE ◽  
MICHELLE M. MOORE ◽  
INSOOK SON ◽  
NELLY TRAN ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Throughput ◽  
Shiga Toxin ◽  
False Positive ◽  
Collaborative Study ◽  
False Negative ◽  
Robust Method ◽  
Suspension Array ◽  
Negative Results ◽  
False Negative Results

Rapid and high-throughput identification and serotyping of Shiga toxin–producing Escherichia coli (STEC) O serogroups is important for detecting, investigating, and controlling STEC infection outbreaks and removing hazardous products from commerce. A Luminex microbead-based suspension array has been developed to identify the 11 most clinically relevant STEC serogroups: O26, O45, O91, O103, O104, O111, O113, O121, O128, O145, and O157. Here we present results of a blinded multilaboratory collaborative study involving 10 participants from nine laboratories using 55 unknown strains. From the total 495 analyses, two false-positive and three false-negative results were obtained, indicating the assay to be a rapid, high-throughput, and robust method for identifying clinically relevant STEC serogroups.

scholarly journals A Robust Method for Quantitative High-throughput Analysis of Proteomes by18O Labeling

2010 ◽  
Vol 10 (1) ◽  
pp. M110.003335 ◽  
Author(s):  
Elena Bonzon-Kulichenko ◽  
Daniel Pérez-Hernández ◽  
Estefanía Núñez ◽  
Pablo Martínez-Acedo ◽  
Pedro Navarro ◽  
...  
Keyword(s):  
High Throughput ◽  
Robust Method ◽  
High Throughput Analysis ◽  
Throughput Analysis
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