In Vitro Model on Glass Surfaces for Complex Interactions between Different Types of Cells

Langmuir ◽  
2010 ◽  
Vol 26 (23) ◽  
pp. 17790-17794 ◽  
Author(s):  
Zhenling Chen ◽  
Wei Chen ◽  
Bo Yuan ◽  
Le Xiao ◽  
Dingbin Liu ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Complex Interactions ◽  
Different Types ◽  
Vitro Model ◽  
Glass Surfaces

In vitro model for the response to irradiation of different types of human intracranial tumours

1987 ◽  
Vol 85 (1-2) ◽  
pp. 46-49 ◽  
Author(s):  
H. Fischer ◽  
G. H. Hartmann ◽  
V. Sturm ◽  
K. Schwechheimer ◽  
O. Krauss ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Intracranial Tumours ◽  
Different Types ◽  
Vitro Model

Peri-Implant Strain in an In Vitro Model

2015 ◽  
Vol 41 (5) ◽  
pp. 532-537 ◽  
Author(s):  
Souheil Hussaini ◽  
Tritala K. Vaidyanathan ◽  
Abhinav P. Wadkar ◽  
Firas A. Al Quran ◽  
David Ehrenberg ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Mechanical Test ◽  
Statistical Treatment ◽  
Test System ◽  
Strain Gages ◽  
Different Types ◽  
Bone Atrophy ◽  
The Mean ◽  
Vitro Model

An in vitro experimental model was designed and tested to determine the influence that peri-implant strain may have on the overall crestal bone. Strain gages were attached to polymethylmethacrylate (PMMA) models containing a screw-type root form implant at sites 1 mm from the resin-implant interface. Three different types of crown superstructures (cemented, 1-screw [UCLA] and 2-screw abutment types) were tested. Loading (1 Hz, 200 N load) was performed using a MTS Mechanical Test System. The strain gage data were stored and organized in a computer for statistical treatment. Strains for all abutment types did not exceed the physiological range for modeling and remodeling of cancellous bone, 200–2500 μɛ (microstrain). For approximately one-quarter of the trials, the strain values were less than 200 μɛ the zone for bone atrophy. The mean microstrain obtained was 517.7 μɛ. In conclusion, the peri-implant strain in this in vitro model did not exceed the physiologic range of bone remodeling under axial occlusal loading.

scholarly journals Effects of homeopathic medicines on macrophage - E. cuniculi interaction in vitro

2021 ◽  
Vol 17 (1) ◽  
pp. 01-01
Author(s):  
Leoni Villano Bonamin ◽  
Mirian Yaeko DO Nagai ◽  
Luciane Costa Dalboni ◽  
Thayná Neves Cardoso ◽  
Michelle S Correia ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Cytokine Production ◽  
Alcohol Concentration ◽  
Encephalitozoon Cuniculi ◽  
Acridine Orange Staining ◽  
Neural Tissues ◽  
Different Types ◽  
Staining Methods ◽  
Vitro Model

Encephalitozoon cuniculi (E. cuniculi) is a fungus that behaves as an intracellular parasite infecting different types of cells. In rabbits and immunosuppressed animals of other species, including humans, it parasites neural tissues causing a highly characteristic neurological syndrome, for which repertory analysis indicates Phosphorus. Successful treatment of 7 naturally infected rabbits with Phosphorus confirmed that this medicine probably is the remedy of the ‘epidemic genius’ of microsporidiosis. In the present study, an in vitro model was used to evidence the intracellular relationship between macrophages and E. cuniculi after treatment of co-cultures with different potencies of Phosphorus. RAW 254.7 macrophages were co-cultivated with E. cuniculi and treated with Phosphorus 6cH, 30cH and 200cH. Controls were untreated co-cultures and cultures treated with vehicle (0.06% final alcohol concentration). Phagocytosis and lysosome activity were after after 1 and 24 hours of incubation by means of the calcofluor and acridine orange staining methods, followed by automatic image analysis (Metamorph®). Cytokine production was assessed with the MAGPIX®-Luminex system. The vehicle increased IL-6, MCP-1 and MIP1 production (p

scholarly journals Levetiracetam and Rufinamide are effective at supressing spike and wave seizure activity in an in vitro model of absence epilepsy

2018 ◽  
Author(s):  
Virdziniha Todorova ◽  
Georgina Ford ◽  
Roger D. Traub ◽  
Miles. A. Whittington ◽  
Stephen. P. Hall
Keyword(s):  
In Vitro Model ◽  
Seizure Activity ◽  
Absence Epilepsy ◽  
Absence Seizures ◽  
Clinical Burden ◽  
Different Types ◽  
Vitro Model ◽  
Atypical Absence ◽  
Component Amplitude

AbstractSpike-and-wave discharges (SpW) are seen in absence-type epilepsies. They are heterogeneous in terms of their clinical burden and their electrographic signature, which is used to classify different types of absence seizures; typical absence, in which SpW frequency is 3-4Hz and atypical absence, which shows a slower 1-2Hz frequency. Treatment of SpW varies dependent upon the syndrome, but both Valproic Acid (VPA) and Ethosuximide (ESM) are shown to be effective in controlling typical absence seizures. Other anti-epileptic’s (AED’s), Levetiracetam (LEV) and Rufinamide (RUF), have shown promise in treating absence epilepsies and their associated syndromes. Here we examine the efficacy of these AED’s on an in vitro model of SpW.Both LEV and RUF show an effective reduction in both the number of SpW events and the spike component amplitude; VPA shows no effect, whilst ESM enhances the spike amplitude. Phenytoin exacerbates the SpW activity, increasing both the number of SpW, amplitude of the SpW and the number of spikes within each event. These data suggest that both LEV and RUF could be effective in the treatment of absence-type epilepsies. They also suggest this model could be an effective tool to test other AED’s aimed at treating atypical absence syndromes.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

A new in vitro model for pre-transplantation diagnosis of frozen/thawed human ovarian tissue

2011 ◽  
Vol 71 (05) ◽  
Author(s):  
M Salama ◽  
K Winkler ◽  
KF Murach ◽  
S Hofer ◽  
L Wildt ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Ovarian Tissue ◽  
Human Ovarian Tissue ◽  
Vitro Model
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