Spatially Controlled Cell Adhesion via Micropatterned Surface Modification of Poly(dimethylsiloxane)

Langmuir ◽  
2007 ◽  
Vol 23 (2) ◽  
pp. 715-719 ◽  
Author(s):  
Natasha Patrito ◽  
Claire McCague ◽  
Peter R. Norton ◽  
Nils O. Petersen
2020 ◽  
Vol 176 ◽  
pp. 109070
Author(s):  
Efraín Rodríguez-Alba ◽  
Nestor Dionisio ◽  
Mitzi Pérez-Calixto ◽  
Lázaro Huerta ◽  
Lorena García-Uriostegui ◽  
...  

2009 ◽  
Vol 20 (12) ◽  
pp. 2541-2548 ◽  
Author(s):  
J. Hauser ◽  
J. Zietlow ◽  
M. Köller ◽  
S. A. Esenwein ◽  
H. Halfmann ◽  
...  

2009 ◽  
Vol 287 (12) ◽  
pp. 1469-1474 ◽  
Author(s):  
Patricia Alves ◽  
Jean-Pierre Kaiser ◽  
Janne Haack ◽  
Natalie Salk ◽  
Arie Bruinink ◽  
...  

2020 ◽  
Vol 21 (24) ◽  
pp. 9679
Author(s):  
Adam Lech ◽  
Beata A. Butruk-Raszeja ◽  
Tomasz Ciach ◽  
Krystyna Lawniczak-Jablonska ◽  
Piotr Kuzmiuk ◽  
...  

Recently, extreme ultraviolet (EUV) radiation has been increasingly used to modify polymers. Properties such as the extremely short absorption lengths in polymers and the very strong interaction of EUV photons with materials may play a key role in achieving new biomaterials. The purpose of the study was to examine the impact of EUV radiation on cell adhesion to the surface of modified polymers that are widely used in medicine: poly(tetrafluoroethylene) (PTFE), poly (vinylidene fluoride) (PVDF), and poly-L-(lactic acid) (PLLA). After EUV surface modification, which has been performed using a home-made laboratory system, changes in surface wettability, morphology, chemical composition and cell adhesion polymers were analyzed. For each of the three polymers, the EUV radiation differently effects the process of endothelial cell adhesion, dependent of the parameters applied in the modification process. In the case of PVDF and PTFE, higher cell number and cellular coverage were obtained after EUV radiation with oxygen. In the case of PLLA, better results were obtained for EUV modification with nitrogen. For all three polymers tested, significant improvements in endothelial cell adhesion after EUV modification have been demonstrated.


Author(s):  
Kateřina Kolářová ◽  
Nikola Kasálková ◽  
Barbora Dvořánková ◽  
Jiří Michálek ◽  
Václav Švorčík

1998 ◽  
Vol 103-104 ◽  
pp. 124-128 ◽  
Author(s):  
Hiroshi Tsuji ◽  
Hiroko Satoh ◽  
Shigeki Ikeda ◽  
Noburo Ikemoto ◽  
Yasuhito Gotoh ◽  
...  

2008 ◽  
Vol 255 (2) ◽  
pp. 459-461 ◽  
Author(s):  
Xiaoyu Li ◽  
Jinfeng Yao ◽  
Xiaojuan Yang ◽  
Weidong Tian ◽  
Lei Liu

2020 ◽  
Author(s):  
Mohammadhossein Dabaghi ◽  
Shadi Shahriari ◽  
Neda Saraei ◽  
Kevin Da ◽  
Abiram Chandiramohan ◽  
...  

AbstractPolydimethylsiloxane (PDMS) is a silicone-based synthetic material that is used in various biomedical applications due to its properties, including transparency, flexibility, permeability to gases, and ease of use. Though PDMS facilitates and realizes the fabrication of complicated geometries at the micro and nano scales, it does not optimally interact with cells for adherence and proliferation. Different strategies have been proposed to render PDMS to enhance cell attachment. The majority of these surface modification techniques have been offered for a static cell culture system. However, dynamic cell culture systems such as organ-on-a-chip devices are demanding platforms that recapitulate the complexity of a living tissue microenvironment. For organ-on-a-chip platforms, PDMS surfaces are usually coated by ECM proteins, which occur as a result of physical, weak bonding between PDMS and ECM proteins, and this binding can be degraded when it is exposed to shear stresses. This work reports static and dynamic coating methods to covalently bind collagen within a PDMS-based microfluidic device using polydopamine (PDA). These coating methods were evaluated using water contact angle measurement and atomic force microscopy (AFM) to find the optimum coating conditions. The biocompatibility of collagen-coated PDMS devices was assessed by culturing primary human bronchial epithelial cells (HBECs) in microfluidic devices. It was shown that both PDA coating methods could be used to bind collagen, thereby improving cell adhesion (around three times higher) without showing any discernible difference. These results suggested that such a surface modification can be used to coat an extracellular matrix protein onto PDMS-based microfluidic devices.


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