Structure and Protein Binding Capacity of a Planar PAA Brush

Langmuir ◽  
2007 ◽  
Vol 23 (3) ◽  
pp. 1347-1353 ◽  
Author(s):  
Oliver Hollmann ◽  
Thomas Gutberlet ◽  
Claus Czeslik
Keyword(s):  
Protein Binding ◽  
Binding Capacity ◽  
Protein Binding Capacity

scholarly journals Impact of Silanization Parameters and Antibody Immobilization Strategy on Binding Capacity of Photonic Ring Resonators

Sensors ◽  
2020 ◽  
Vol 20 (11) ◽  
pp. 3163
Author(s):  
Nina Bjørk Arnfinnsdottir ◽  
Cole A. Chapman ◽  
Ryan C. Bailey ◽  
Astrid Aksnes ◽  
Bjørn Torger Stokke
Keyword(s):  
Protein Binding ◽  
Ring Resonator ◽  
Silicon Oxide ◽  
Binding Capacity ◽  
Ring Resonators ◽  
Widespread Application ◽  
Reactive Protein ◽  
Oxide Substrates ◽  
Protein Binding Capacity ◽  
Selection Of

Ring resonator-based biosensors have found widespread application as the transducing principle in “lab-on-a-chip” platforms due to their sensitivity, small size and support for multiplexed sensing. Their sensitivity is, however, not inherently selective towards biomarkers, and surface functionalization of the sensors is key in transforming the sensitivity to be specific for a particular biomarker. There is currently no consensus on process parameters for optimized functionalization of these sensors. Moreover, the procedures are typically optimized on flat silicon oxide substrates as test systems prior to applying the procedure to the actual sensor. Here we present what is, to our knowledge, the first comparison of optimization of silanization on flat silicon oxide substrates to results of protein capture on sensors where all parameters of two conjugation protocols are tested on both platforms. The conjugation protocols differed in the chosen silanization solvents and protein immobilization strategy. The data show that selection of acetic acid as the solvent in the silanization step generally yields a higher protein binding capacity for C-reactive protein (CRP) onto anti-CRP functionalized ring resonator sensors than using ethanol as the solvent. Furthermore, using the BS3 linker resulted in more consistent protein binding capacity across the silanization parameters tested. Overall, the data indicate that selection of parameters in the silanization and immobilization protocols harbor potential for improved biosensor binding capacity and should therefore be included as an essential part of the biosensor development process.

scholarly journals Protein Binding Capacity of Different Forages Tannin

2018 ◽  
Vol 119 ◽  
pp. 012007 ◽  
Author(s):  
L. M. Yusiati ◽  
A. Kurniawati ◽  
C. Hanim ◽  
M. A. Anas
Keyword(s):  
Protein Binding ◽  
Binding Capacity ◽  
Protein Binding Capacity

Preparation and characterization of magnetic polymer nanospheres with high protein binding capacity

2005 ◽  
Vol 293 (1) ◽  
pp. 111-118 ◽  
Author(s):  
Xianqiao Liu ◽  
Yueping Guan ◽  
Huizhou Liu ◽  
Zhiya Ma ◽  
Yu Yang ◽  
...  
Keyword(s):  
Protein Binding ◽  
Binding Capacity ◽  
High Protein ◽  
High Protein Binding ◽  
Protein Binding Capacity ◽  
Polymer Nanospheres

A modified method for determining protein binding capacity of plant polyphenolics using radiolabelled protein

2004 ◽  
Vol 15 (3) ◽  
pp. 159-163 ◽  
Author(s):  
Ginger L. Henson ◽  
Lee Niemeyer ◽  
Godfred Ansong ◽  
Rebecca Forkner ◽  
Harinder P. S. Makkar ◽  
...  
Keyword(s):  
Protein Binding ◽  
Binding Capacity ◽  
Modified Method ◽  
Protein Binding Capacity
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