Multicomponent Cationic Lipid−DNA Complex Formation:  Role of Lipid Mixing

Langmuir ◽  
2005 ◽  
Vol 21 (25) ◽  
pp. 11582-11587 ◽  
Author(s):  
Giulio Caracciolo ◽  
Daniela Pozzi ◽  
Heinz Amenitsch ◽  
Ruggero Caminiti
Keyword(s):  
Complex Formation ◽  
Cationic Lipid ◽  
Dna Complex

scholarly journals Cryo-EM structure ofEscherichia coliσ70RNA polymerase and promoter DNA complex revealed a role of σ non-conserved region during the open complex formation

2018 ◽  
Vol 293 (19) ◽  
pp. 7367-7375 ◽  
Author(s):  
Anoop Narayanan ◽  
Frank S. Vago ◽  
Kunpeng Li ◽  
M. Zuhaib Qayyum ◽  
Dinesh Yernool ◽  
...  
Keyword(s):  
Complex Formation ◽  
Conserved Region ◽  
Open Complex Formation ◽  
Promoter Dna ◽  
Dna Complex

Modelling of DNA Complexes with Distamycin Analogues Using an ab initio Continuum Solvent Model

2000 ◽  
Vol 65 (5) ◽  
pp. 631-643 ◽  
Author(s):  
Petr Bouř ◽  
Vladimír Král
Keyword(s):  
Complex Formation ◽  
Ab Initio ◽  
Polar Solvent ◽  
Molecular Shape ◽  
Conformational Flexibility ◽  
Model Systems ◽  
Dna Complexes ◽  
Solvent Model ◽  
Dna Complex

Model systems related to non-covalent minor groove DNA complexes with distamycin analogues were investigated using the Turbomole and Gaussian quantum chemical packages. The role of molecular shape, electrostatic field and conformer energies in the complex formation was discussed. The ab initio calculations included the COSMO solvent model. If compared to vacuum computations, polar solvent significantly destabilizes such complexes and increases conformational flexibility of distamycin. The DNA complex formation appears to be driven mainly by entropy lowering and complementarity of molecular shapes. The NH moiety of the amide group preferably points to the base pair according to the computations, in agreement with experimental data.

scholarly journals 2P117 Thermodynamics of protein-DNA complex formation in relation to structure

2004 ◽  
Vol 44 (supplement) ◽  
pp. S139
Author(s):  
H. Uedaira ◽  
H. Kono ◽  
Ponraj Prabakaran ◽  
K. Kitajima ◽  
A. Sarai
Keyword(s):  
Complex Formation ◽  
Dna Complex

scholarly journals An optical biosensor study of the interaction parameters and role of hydrophobic tails of cytochrome P450 2B4, b5 and NADPH-flavoprotein in complex formation

IUBMB Life ◽  
1997 ◽  
Vol 42 (4) ◽  
pp. 731-737 ◽  
Author(s):  
Yuriy Ivanov ◽  
Irina Kanaeva ◽  
Michail Eldarov ◽  
Konstantin Sklyabin ◽  
Michael Lehnerer ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Complex Formation ◽  
Optical Biosensor ◽  
Interaction Parameters ◽  
Cytochrome P450 2B4

scholarly journals The Regulator of Nitrate Assimilation in Ascomycetes Is a Dimer Which Binds a Nonrepeated, Asymmetrical Sequence

1998 ◽  
Vol 18 (3) ◽  
pp. 1339-1348 ◽  
Author(s):  
Joseph Strauss ◽  
M. Isabel Muro-Pastor ◽  
Claudio Scazzocchio
Keyword(s):  
Nitrate Assimilation ◽  
Binding Studies ◽  
Binding Ability ◽  
Zinc Cluster ◽  
First Case ◽  
Vitro Binding ◽  
Nitrate Induction ◽  
Dna Complex

ABSTRACT The regulation of nitrate assimilation seems to follow the same pattern in all ascomycetes where this process has been studied. We show here by in vitro binding studies and a number of protection and interference techniques that the transcription factor mediating nitrate induction in Aspergillus nidulans, a protein containing a binuclear zinc cluster DNA binding domain, recognizes an asymmetrical sequence of the form CTCCGHGG. We further show that the protein binds to its consensus site as a dimer. We establish the role of the putative dimerization element by its ability to replace the analogous element of the cI protein of phage λ. Mutagenesis of crucial leucines of the dimerization element affect both the binding ability of the dimer and the conformation of the resulting protein-DNA complex. This is the first case to be described where a dimer recognizes such an asymmetrical nonrepeated sequence, presumably by each monomeric subunit making different contacts with different DNA half-sites.

scholarly journals Impact of Quantum Dot Surface on Complex Formation with Chlorin e6 and Photodynamic Therapy

Nanomaterials ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 9 ◽  
Author(s):  
Artiom Skripka ◽  
Dominyka Dapkute ◽  
Jurga Valanciunaite ◽  
Vitalijus Karabanovas ◽  
Ricardas Rotomskis
Keyword(s):  
Photodynamic Therapy ◽  
Cancer Therapy ◽  
Complex Formation ◽  
Chemical Properties ◽  
Disease Diagnosis ◽  
Chlorin E6 ◽  
Deep Tissue ◽  
Physico Chemical

Nanomaterials have permeated various fields of scientific research, including that of biomedicine, as alternatives for disease diagnosis and therapy. Among different structures, quantum dots (QDs) have distinctive physico-chemical properties sought after in cancer research and eradication. Within the context of cancer therapy, QDs serve the role of transporters and energy donors to photodynamic therapy (PDT) drugs, extending the applicability and efficiency of classic PDT. In contrast to conventional PDT agents, QDs’ surface can be designed to promote cellular targeting and internalization, while their spectral properties enable better light harvesting and deep-tissue use. Here, we investigate the possibility of complex formation between different amphiphilic coating bearing QDs and photosensitizer chlorin e6 (Ce6). We show that complex formation dynamics are dependent on the type of coating—phospholipids or amphiphilic polymers—as well as on the surface charge of QDs. Förster’s resonant energy transfer occurred in every complex studied, confirming the possibility of indirect Ce6 excitation. Nonetheless, in vitro PDT activity was restricted only to negative charge bearing QD-Ce6 complexes, correlating with better accumulation in cancer cells. Overall, these findings help to better design such and similar complexes, as gained insights can be straightforwardly translated to other types of nanostructures—expanding the palette of possible therapeutic agents for cancer therapy.

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